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![Western Blot: NEDD9/CASL/HEF1 Antibody (2G9) - (82-398 aa) [NB100-1699] - Detection of a 115 kDa band corresponding to HEF1 in MCF7 lysate (arrowhead). Approximately 35 ig of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-2G9-82-398-aa-Western-Blot-NB100-1699-img0011.jpg "Western Blot: NEDD9/CASL/HEF1 Antibody (2G9) - (82-398 aa) [NB100-1699] - Detection of a 115 kDa band corresponding to HEF1 in MCF7 lysate (arrowhead). Approximately 35 ig of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody (2G9) - (82-398 aa) [NB100-1699] - Detection of HEF1 localized at focal adhesion sites. The antibody was used at a 1:500 dilution with a 3-sec exposure time.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-2G9-82-398-aa-Immunocytochemistry-Immunofluorescence-NB100-1699-img0009.jpg)
![Western Blot: NEDD9/CASL/HEF1 Antibody (2G9) - (82-398 aa) [NB100-1699] - Detection of endogenous HEF1 present in various cell lines. Panel A shows detection using a 15 min exposure. Panel B is the same blot exposed for 2 min. The doublet represents p105 and p115 staining. The lower MW band represents p55. 3Y1 cells are derived from rat fibroblast cells. Mouse 3T3 cells are also reactive (not shown). To date no staining has been noted on CHO cells.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-2G9-82-398-aa-Western-Blot-NB100-1699-img0010.jpg)
![Western Blot: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01] - Analysis of NEDD9 expression in transfected 293T cell line by NEDD9 monoclonal antibody (M01), clone 1B4.Lane 1: NEDD9 transfected lysate(92.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-1B4-Western-Blot-H00004739-M01-img0003.jpg "Western Blot: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01] - Analysis of NEDD9 expression in transfected 293T cell line by NEDD9 monoclonal antibody (M01), clone 1B4.Lane 1: NEDD9 transfected lysate(92.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody (1B4) [H00004739-M01] - Analysis of monoclonal antibody to NEDD9 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-1B4-Immunocytochemistry-Immunofluorescence-H00004739-M01-img0002.jpg)
![Western Blot: NEDD9/CASL/HEF1 Antibody (14A11) - (82-398 aa) [NBP2-44192] - Analysis of anti-HEF1 antibody (clone 14A11) shows detection of HEF1 present in MCF-7 cells induced to express HEF1 by tetracycline removal (right lane).](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-14A11-82-398-aa-Western-Blot-NBP2-44192-img0003.jpg "Western Blot: NEDD9/CASL/HEF1 Antibody (14A11) - (82-398 aa) [NBP2-44192] - Analysis of anti-HEF1 antibody (clone 14A11) shows detection of HEF1 present in MCF-7 cells induced to express HEF1 by tetracycline removal (right lane).")
![Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody (14A11) - (82-398 aa) [NBP2-44192] - Detection of HEF1 localized at the centrosome (bright dots) and focal adhesion sites. The antibody was used at a 1:100 dilution with a 1-min exposure time.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-14A11-82-398-aa-Immunocytochemistry-Immunofluorescence-NBP2-44192-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody (14A11) - (82-398 aa) [NBP2-44192] - Detection of HEF1 localized at focal adhesion sites. Per Elena Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-14A11-82-398-aa-Immunocytochemistry-Immunofluorescence-NBP2-44192-img0001.jpg)
![Western Blot: NEDD9/CASL/HEF1 Antibody [NBP2-55071] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-Western-Blot-NBP2-55071-img0002.jpg "Western Blot: NEDD9/CASL/HEF1 Antibody [NBP2-55071] - Analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody [NBP2-55071] - Staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55071-img0001.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT4 | Lane 3: Human Plasma | Lane 4: U-251 MG | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21227_rabbit-nedd9-casl-hef1-pab-4520231550314.jpg "Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT4 | Lane 3: Human Plasma | Lane 4: U-251 MG | Lane 5: Liver | Lane 6: Tonsil")
![Western Blot: NEDD9/CASL/HEF1 Antibody [NB100-68151] - Staining of Human Kidney lysate.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-Western-Blot-NB100-68151-img0004.jpg "Western Blot: NEDD9/CASL/HEF1 Antibody [NB100-68151] - Staining of Human Kidney lysate.")
![Immunohistochemistry-Paraffin: NEDD9/CASL/HEF1 Antibody [NBP2-37887] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-Immunohistochemistry-NBP2-37887-img0001.jpg "Immunohistochemistry-Paraffin: NEDD9/CASL/HEF1 Antibody [NBP2-37887] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: NEDD9/CASL/HEF1 Antibody [NBP3-17640] - Staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NEDD9-CASL-HEF1-Antibody-Immunohistochemistry-Paraffin-NBP3-17640-img0002.jpg "Immunohistochemistry-Paraffin: NEDD9/CASL/HEF1 Antibody [NBP3-17640] - Staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.")