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![Western Blot: NDUFB7 Antibody [NBP2-94617] - Analysis of extracts of various cell lines, using NDUFB7 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Western-Blot-NBP2-94617-img0005.jpg "Western Blot: NDUFB7 Antibody [NBP2-94617] - Analysis of extracts of various cell lines, using NDUFB7 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: NDUFB7 Antibody [NBP2-94617] - Analysis of L929 cells using NDUFB7 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94617-img0001.jpg)
![Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-94617] - Paraffin-embedded rat heart using NDUFB7 .](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunohistochemistry-Paraffin-NBP2-94617-img0004.jpg)
![Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-94617] - Paraffin-embedded human esophageal using NDUFB7 .](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunohistochemistry-Paraffin-NBP2-94617-img0002.jpg)
![Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-94617] - Paraffin-embedded mouse spleen using NDUFB7 .](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunohistochemistry-Paraffin-NBP2-94617-img0003.jpg)
![Western Blot: NDUFB7 Antibody [NBP2-97854] - Anti-NDUFB7 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate Lane B: HL60 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 19 kDa](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Western-Blot-NBP2-97854-img0006.jpg "Western Blot: NDUFB7 Antibody [NBP2-97854] - Anti-NDUFB7 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate Lane B: HL60 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 19 kDa")
![Immunocytochemistry/Immunofluorescence: NDUFB7 Antibody [NBP2-97854] - Immunofluorescence staining of NDUFB7 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NDUFB7 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97854-img0002.jpg)
![Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854] - Immunochemical staining of human NDUFB7 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunohistochemistry-Paraffin-NBP2-97854-img0005.jpg)
![Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP2-97854] - Immunochemical staining of human NDUFB7 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunohistochemistry-Paraffin-NBP2-97854-img0004.jpg)
![Immunoprecipitation: NDUFB7 Antibody [NBP2-97854] - NDUFB7 was immunoprecipitated using: Lane A: 0.5 mg HL60 Whole Cell Lysate 4 ul anti-NDUFB7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDUFB7 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunoprecipitation-NBP2-97854-img0007.jpg)
![Western Blot: NDUFB7 Antibody [NBP1-88938] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Western-Blot-NBP1-88938-img0003.jpg "Western Blot: NDUFB7 Antibody [NBP1-88938] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).")
![Immunohistochemistry-Paraffin: NDUFB7 Antibody [NBP1-88938] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Immunohistochemistry-Paraffin-NBP1-88938-img0001.jpg)
![Western Blot: NDUFB7 Antibody [NBP1-88938] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/NDUFB7-Antibody-Western-Blot-NBP1-88938-img0004.jpg)
![Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - Analysis of NDUFB7 expression in transfected 293T cell line by NDUFB7 monoclonal antibody (M01), clone 4D4.Lane 1: NDUFB7 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-4D4-Western-Blot-H00004713-M01-img0008.jpg "Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - Analysis of NDUFB7 expression in transfected 293T cell line by NDUFB7 monoclonal antibody (M01), clone 4D4.Lane 1: NDUFB7 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.")
![<b>Genetic Strategies Validation. </b>Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - Analysis of NDUFB7 over-expressed 293 cell line, cotransfected with NDUFB7 Validated Chimera RNAi ( Cat # H00004713-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFB7 monoclonal antibody (M01), clone 4D4 (Cat # H00004713-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-4D4-Western-Blot-H00004713-M01-img0005.jpg)
![Western Blot: NDUFB7 Antibody (4D4) [H00004713-M01] - NDUFB7 monoclonal antibody (M01), clone 4D4 Analysis of NDUFB7 expression in A-431.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-4D4-Western-Blot-H00004713-M01-img0007.jpg)
![ELISA: NDUFB7 Antibody (4D4) [H00004713-M01] - Detection limit for recombinant GST tagged NDUFB7 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NDUFB7-Antibody-4D4-ELISA-H00004713-M01-img0009.jpg)
![Western Blot: NDUFB7 Overexpression Lysate (Adult Normal) [NBL1-13555] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDUFB7.](http://images.novusbio.com/fullsize/NDUFB7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13555-img0002.jpg "Western Blot: NDUFB7 Overexpression Lysate (Adult Normal) [NBL1-13555] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDUFB7.")