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![Western Blot: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Western blot analysis of Alpha-mannosidase II on different lysates with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: JAR cell lysate Lane 4:U-87 MG cell lysate Lane 5: HUVEC cell lysate Lane 6: A375 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: NCI-H226 cell lysate Lane 9: Human liver tissue lysate Lane 10: human lung tissue lysate Lane 11: human kidney tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 131 kDa Observed band size: 131-151 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-27820241631657.jpg "Western Blot: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Western blot analysis of Alpha-mannosidase II on different lysates with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: JAR cell lysate Lane 4:U-87 MG cell lysate Lane 5: HUVEC cell lysate Lane 6: A375 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: NCI-H226 cell lysate Lane 9: Human liver tissue lysate Lane 10: human lung tissue lysate Lane 11: human kidney tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 131 kDa Observed band size: 131-151 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32565) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Alpha-mannosidase II antibody (NBP3-32565) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-29820241420970.jpg)
![Flow Cytometry: Alpha-mannosidase II Antibody (PSH02-67) [NBP3-32565] - Flow cytometric analysis of U-87 MG cells labeling Alpha-mannosidase II. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32565, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32565_rabbit-alpha-mannosidase-ii-mab-psh02-67-29202416372118.jpg)
![Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Immunohistochemistry-Paraffin-NBP1-89748-img0007.jpg "Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human skeletal muscle shows low expression as expected.")
![Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Immunohistochemistry-Paraffin-NBP1-89748-img0005.jpg)
![Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Immunohistochemistry-Paraffin-NBP1-89748-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Alpha-mannosidase II Antibody [NBP1-89748] - Staining in human parathyroid gland and skeletal muscle tissues using anti-MAN2A1 antibody. Corresponding MAN2A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Immunohistochemistry-Paraffin-NBP1-89748-img0008.jpg)
![Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - HEK293 overexpressing Man2A1 and probed with this antibody (mock transfection in first lane).](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Western-Blot-NB100-93414-img0005.jpg "Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - HEK293 overexpressing Man2A1 and probed with this antibody (mock transfection in first lane).")
![Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - Analysis of alpha-mannosidase II antibody [NB100-93414] (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-Western-Blot-NB100-93414-img0004.jpg)
![Western Blot: Alpha-mannosidase II Antibody (1G9) [H00004124-M01] - Detection against Immunogen (36.74 KDa) .](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Antibody-1G9-Western-Blot-H00004124-M01-img0001.jpg "Western Blot: Alpha-mannosidase II Antibody (1G9) [H00004124-M01] - Detection against Immunogen (36.74 KDa) .")
![Western Blot: MAN2A1 Antibody [NBP2-92006] - Analysis of extracts of various cell lines, using MAN2A1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/MAN2A1-Antibody-Western-Blot-NBP2-92006-img0001.jpg "Western Blot: MAN2A1 Antibody [NBP2-92006] - Analysis of extracts of various cell lines, using MAN2A1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Western Blot: Alpha-mannosidase II Overexpression Lysate (Adult Normal) [NBL1-12834] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha-mannosidase II.](http://images.novusbio.com/fullsize/Alpha-mannosidase-II-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12834-img0002.jpg "Western Blot: Alpha-mannosidase II Overexpression Lysate (Adult Normal) [NBL1-12834] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha-mannosidase II.")