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![Western Blot: dUTPase Antibody [NBP2-33277] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/dUTPase-Antibody-Western-Blot-NBP2-33277-img0005.jpg "Western Blot: dUTPase Antibody [NBP2-33277] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: dUTPase Antibody [NBP2-33277] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33277-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining in human lymph node and skeletal muscle tissues using anti-DUT antibody. Corresponding DUT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0009.jpg)
![Immunohistochemistry: dUTPase Antibody [NBP2-33277] - Staining of human tonsil shows strong cytoplasmic and nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-NBP2-33277-img0002.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining of liver.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0003.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining of liver cancer.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0004.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0007.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-33277] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-33277-img0008.jpg)
![Western Blot: dUTPase Antibody [NBP2-92887] - Analysis of extracts of various cell lines, using dUTPase at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Western-Blot-NBP2-92887-img0005.jpg "Western Blot: dUTPase Antibody [NBP2-92887] - Analysis of extracts of various cell lines, using dUTPase at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: dUTPase Antibody [NBP2-92887] - Analysis of U-2 OS cells using dUTPase . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92887-img0001.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-92887] - Paraffin-embedded rat spleen using dUTPase .](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-92887-img0004.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-92887] - Paraffin-embedded human appendix using dUTPase .](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-92887-img0002.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-92887] - Paraffin-embedded human liver cancer using dUTPase .](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-92887-img0003.jpg)
![Western Blot: dUTPase Antibody (JE66-11) [NBP3-32281] - Western blot analysis of dUTPase on different lysates with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: A549 cell lysate (15 ug/Lane) Lane 3: Jurkat cell lysate (15 ug/Lane) Lane 4: LO2 cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: K-562 cell lysate (15 ug/Lane) Lane 7: HEK-293 cell lysate (15 ug/Lane) Lane 8: A431 cell lysate (15 ug/Lane) Lane 9: Human liver tissue lysate (30 ug/Lane) Predicted band size: 27 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-278202415295584.jpg "Western Blot: dUTPase Antibody (JE66-11) [NBP3-32281] - Western blot analysis of dUTPase on different lysates with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 ug/Lane) Lane 2: A549 cell lysate (15 ug/Lane) Lane 3: Jurkat cell lysate (15 ug/Lane) Lane 4: LO2 cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: K-562 cell lysate (15 ug/Lane) Lane 7: HEK-293 cell lysate (15 ug/Lane) Lane 8: A431 cell lysate (15 ug/Lane) Lane 9: Human liver tissue lysate (30 ug/Lane) Predicted band size: 27 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: dUTPase Antibody (JE66-11) [NBP3-32281] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32281) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29820241430414.jpg)
![Immunocytochemistry/ Immunofluorescence: dUTPase Antibody (JE66-11) [NBP3-32281] - Immunocytochemistry analysis of HCT116 cells labeling dUTPase with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-dUTPase antibody (NBP3-32281) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29202410334031.jpg)
![Flow Cytometry: dUTPase Antibody (JE66-11) [NBP3-32281] - Flow cytometric analysis of HCT116 cells labeling dUTPase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32281, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32281_rabbit-dutpase-mab-je66-11-29202416372165.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-16241] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-16241-img0002.jpg "Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-16241] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.")
![Immunoprecipitation: dUTPase Antibody [NBP2-16241] - Immunoprecipitation of DUT protein from A431 whole cell extracts using 5 ug of DUT antibody. Western blot analysis was performed using DUT antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunoprecipitation-NBP2-16241-img0004.jpg)
![Western Blot: dUTPase Antibody [NBP2-16241] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with dUTPase antibody (NBP2-16241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16241_rabbit-polyclonal-dutpase-antibody-91020242014671.jpg)
![Western Blot: dUTPase Antibody [NB100-56624] - Detection of DUT-N in HeLa cell lysate using this antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Western-Blot-NB100-56624-img0002.jpg "Western Blot: dUTPase Antibody [NB100-56624] - Detection of DUT-N in HeLa cell lysate using this antibody at a dilution of 1:1000.")
![Immunohistochemistry-Paraffin: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - DUT antibody [HL1210] detects DUT protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human A549. DUT stained by DUT antibody [HL1210] (NBP3-25445) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25445_rabbit-dutpase-mab-hl1210-azide-and-bsa-free-2120249415978.jpg "Immunohistochemistry-Paraffin: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - DUT antibody [HL1210] detects DUT protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human A549. DUT stained by DUT antibody [HL1210] (NBP3-25445) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with DUT antibody [HL1210] (NBP3-25445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25445_rabbit-dutpase-mab-hl1210-azide-and-bsa-free-2120249351376.jpg)
![Immunocytochemistry/Immunofluorescence: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - DUT antibody [HL1210] detects DUT protein at cytoplasm, nucleus and centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DUT stained by DUT antibody [HL1210] (NBP3-25445) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25445_rabbit-dutpase-mab-hl1210-azide-and-bsa-free-2120249362715.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-47479] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-47479-img0004.jpg "Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-47479] - Staining of human lymph node shows high expression.")
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-47479] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-47479-img0002.jpg)
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-47479] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-47479-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-47479] - Staining in human lymph node and liver tissues using anti-DUT antibody. Corresponding DUT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-47479-img0005.jpg)
![Western Blot: dUTPase Antibody (SR1280) [NBP3-22634] - Western blot analysis using NBP3-22634 in Ramos cell lysate.](http://images.novusbio.com/fullsize/nbp3-22634_rabbit-dutpase-mab-sr1280-5720231564713.jpg "Western Blot: dUTPase Antibody (SR1280) [NBP3-22634] - Western blot analysis using NBP3-22634 in Ramos cell lysate.")
![Western Blot: dUTPase Overexpression Lysate (Adult Normal) [NBL1-10060] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for dUTPase.](http://images.novusbio.com/fullsize/dUTPase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10060-img0002.jpg "Western Blot: dUTPase Overexpression Lysate (Adult Normal) [NBL1-10060] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for dUTPase.")
![SDS-Page: Recombinant Human dUTPase Protein [NBP1-48598] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-dUTPase-Protein-SDS-Page-NBP1-48598-img0001.jpg "SDS-Page: Recombinant Human dUTPase Protein [NBP1-48598] - 15 % SDS-PAGE (3ug)")
![Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-97744] - Immunochemical staining of human dUTPase in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/dUTPase-Antibody-Immunohistochemistry-Paraffin-NBP2-97744-img0001.jpg "Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-97744] - Immunochemical staining of human dUTPase in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")