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![Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-13881] - Staining in human bone marrow and skeletal muscle tissues using NBP2-13881 antibody. Corresponding CST7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-13881-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-13881] - Staining in human bone marrow and skeletal muscle tissues using NBP2-13881 antibody. Corresponding CST7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-13881] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-13881-img0007.jpg)
![Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-13881] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-13881-img0010.jpg)
![Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-13881] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-13881-img0011.jpg)
![Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-13881] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-13881-img0012.jpg)
![Western Blot: Cystatin F Antibody [NBP2-92532] - Analysis of extracts of various cell lines, using Cystatin F .Exposure time: 90s.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Western-Blot-NBP2-92532-img0001.jpg "Western Blot: Cystatin F Antibody [NBP2-92532] - Analysis of extracts of various cell lines, using Cystatin F .Exposure time: 90s.")
![Immunohistochemistry: Cystatin F Antibody [NBP2-92532] - Immunofluorescence analysis of Mouse spleen using Cystatin F antibody (NBP2-92532) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Immunohistochemistry-NBP2-92532-img0002.jpg)
![Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074] - Analysis of paraffin-embedded CL1-5 xenograft, using cystatin F antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Immunohistochemistry-Paraffin-NBP2-16074-img0002.jpg "Immunohistochemistry-Paraffin: Cystatin F Antibody [NBP2-16074] - Analysis of paraffin-embedded CL1-5 xenograft, using cystatin F antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunocytochemistry/ Immunofluorescence: Cystatin F Antibody [NBP2-16074] - Cystatin F antibody detects Cystatin F protein at vesicle by immunofluorescent analysis.Sample: HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cystatin F stained by Cystatin F antibody (NBP2-16074) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16074_rabbit-polyclonal-cystatin-f-antibody-910202420131263.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cystatin F Antibody [NBP2-16074] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin F antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16074_rabbit-polyclonal-cystatin-f-antibody-1610202419414572.jpg)
![Western Blot: Cystatin F Antibody [NBP2-99007] - Anti-Cystatin F rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Western-Blot-NBP2-99007-img0002.jpg "Western Blot: Cystatin F Antibody [NBP2-99007] - Anti-Cystatin F rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 20 kDa")
![Sandwich ELISA: Cystatin F Antibody Pair [HRP] [NBP2-79460] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Cystatin-F-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79460-img0001.jpg "Sandwich ELISA: Cystatin F Antibody Pair [HRP] [NBP2-79460] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")