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![Western Blot: CRYBA1 Antibody [NBP1-33010] - The CRYBA1 p.G91del mutation reduced its expression in two cell lines. The protein levels of CRYBA1 were measured by Western blot. In 293T and SRA cells, the exogenous CRYBA1 was detected using anti-FLAG antibody; In SRA cells, the general CRYBA1 protein level was also measured using anti-CRYBA1 antibody. beta-Actin or GAPDH were used as internal control. NC, negative control: transfection reagent only. Image collected and cropped by CiteAb from the following publication (bmcmedgenet.biomedcentral.com/articles/10.1186/s12881-019-0882-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CRYBA1-Antibody-Western-Blot-NBP1-33010-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CRYBA1 Antibody [NBP1-33010] - The CRYBA1 p.G91del mutation reduced its expression in two cell lines. The protein levels of CRYBA1 were measured by Western blot. In 293T and SRA cells, the exogenous CRYBA1 was detected using anti-FLAG antibody; In SRA cells, the general CRYBA1 protein level was also measured using anti-CRYBA1 antibody. beta-Actin or GAPDH were used as internal control. NC, negative control: transfection reagent only. Image collected and cropped by CiteAb from the following publication (bmcmedgenet.biomedcentral.com/articles/10.1186/s12881-019-0882-z), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: CRYBA1 Antibody [NBP1-33010] - A. 50 ug mouse fetal eye lysate/extract 12 % SDS-PAGE beta A3 Crystallin antibody dilution: 1:10000](http://images.novusbio.com/fullsize/CRYBA1-Antibody-Western-Blot-NBP1-33010-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CRYBA1 Antibody [NBP1-33010] - Immunofluorescence staining of exogenous WT & mutated CRYBA1 in 2 cell lines. After the WT & deleted forms of CRYBA1 cDNA constructs were transfected into 293 T & SRA lines, cells were stained with FLAG primary antibody. p.G91del mutation led to greater aggregation of the protein at the cell membrane. Blue: nuclear; green: exogenous CRYBA1, scale bar: 20 μM Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31488069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33010_rabbit-polyclonal-cryba1-antibody-310202415334916.jpg)
![Western Blot: CRYBA1 Antibody [NBP1-33010] - CRYBA1 is expressed less in cataract lens epithelium. (a) Western blot images of CRYBA1 in ARC & normal control lens epithelium. Three samples from each group are presented. beta -Actin was used as an internal control. (b) Quantification of the gel images of the Western blots in (a) (n = 3). (c) The mRNA level of CRYBA1 was measured by qPCR in ARC & normal control lens epithelium. beta -Actin was used as an internal control (n = 3). Student’s t test, *P < 0.05, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31488069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33010_rabbit-polyclonal-cryba1-antibody-31020241534397.jpg)
![SDS-Page: Recombinant Human CRYBA1 Protein [H00001411-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001411-P01-1.jpg "SDS-Page: Recombinant Human CRYBA1 Protein [H00001411-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: CRYBA1 Antibody [NBP2-68596] - Immunohistochemical staining of human eye shows strong positivity in lens.](http://images.novusbio.com/fullsize/CRYBA1-Antibody-Immunohistochemistry-NBP2-68596-img0001.jpg "Immunohistochemistry-Paraffin: CRYBA1 Antibody [NBP2-68596] - Immunohistochemical staining of human eye shows strong positivity in lens.")
![Western Blot: CRYBA1 Antibody [NBP1-52907] - HT1080 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CRYBA1-Antibody-Western-Blot-NBP1-52907-img0002.jpg "Western Blot: CRYBA1 Antibody [NBP1-52907] - HT1080 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CRYBA1 Antibody [NBP2-87214] - Host: Rabbit. Target Name: CRBA1. Sample Type: 721_B Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CRYBA1-Antibody-Western-Blot-NBP2-87214-img0001.jpg "Western Blot: CRYBA1 Antibody [NBP2-87214] - Host: Rabbit. Target Name: CRBA1. Sample Type: 721_B Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: CRYBA1 Overexpression Lysate (Adult Normal) [NBP2-07777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRYBA1.](http://images.novusbio.com/fullsize/CRYBA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07777-img0001.jpg "Western Blot: CRYBA1 Overexpression Lysate (Adult Normal) [NBP2-07777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CRYBA1.")