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![Western Blot: CACYBP Antibody [NBP1-87104] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/CACYBP-Antibody-Western-Blot-NBP1-87104-img0007.jpg "Western Blot: CACYBP Antibody [NBP1-87104] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: CACYBP Antibody [NBP1-87104] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/CACYBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87104-img0009.jpg)
![Immunohistochemistry-Paraffin: CACYBP Antibody [NBP1-87104] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/CACYBP-Antibody-Immunohistochemistry-Paraffin-NBP1-87104-img0002.jpg)
![Western Blot: CACYBP Antibody [NBP1-87104] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/CACYBP-Antibody-Western-Blot-NBP1-87104-img0006.jpg)
![Western Blot: CACYBP Antibody [NBP2-15660] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CACYBP-Antibody-Western-Blot-NBP2-15660-img0001.jpg "Western Blot: CACYBP Antibody [NBP2-15660] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CACYBP Antibody [NBP2-15660] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CACYBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15660-img0003.jpg)
![Immunohistochemistry-Paraffin: CACYBP Antibody [NBP2-15660] - Analysis of paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CACYBP-Antibody-Immunohistochemistry-Paraffin-NBP2-15660-img0004.jpg)
![Western Blot: CACYBP Antibody [NBP1-49922] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody NBP1-49922 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody NBP1-49921, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/CACYBP-Antibody-Western-Blot-NBP1-49922-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CACYBP Antibody [NBP1-49922] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody NBP1-49922 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody NBP1-49921, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: CACYBP Antibody [NBP1-49922] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- CacyBP used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CACYBP-Antibody-Immunohistochemistry-NBP1-49922-img0003.jpg)
![Western Blot: CACYBP Antibody (2E3) [H00027101-M01] - Analysis of CACYBP expression in transfected 293T cell line by CACYBP monoclonal antibody (M01), clone 2E3.Lane 1: CACYBP transfected lysate(26.61 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CACYBP-Antibody-2E3-Western-Blot-H00027101-M01-img0005.jpg "Western Blot: CACYBP Antibody (2E3) [H00027101-M01] - Analysis of CACYBP expression in transfected 293T cell line by CACYBP monoclonal antibody (M01), clone 2E3.Lane 1: CACYBP transfected lysate(26.61 KDa).Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: CACYBP Antibody (2E3) [H00027101-M01] - Analysis of protein-protein interactions between SKP1 and CACYBP. HeLa cells were stained with anti-SKP1 rabbit purified polyclonal 1:1200 and anti-CACYBP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CACYBP-Antibody-2E3-Proximity-Ligation-Assay-H00027101-M01-img0003.jpg)
![Sandwich ELISA: CACYBP Antibody (2E3) [H00027101-M01] - Detection limit for recombinant GST tagged CACYBP is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CACYBP-Antibody-2E3-Sandwich-ELISA-H00027101-M01-img0004.jpg)
![Western Blot: CACYBP Antibody (3G8) [NBP2-42677] - The cell lysates of HeLa, 293T, Jurkat and A549(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CACYBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/CACYBP-Antibody-3G8-Western-Blot-NBP2-42677-img0001.jpg "Western Blot: CACYBP Antibody (3G8) [NBP2-42677] - The cell lysates of HeLa, 293T, Jurkat and A549(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CACYBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: CACYBP Antibody [NBP1-49921] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody NBP1-49921 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody NBP1-49922, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/CACYBP-Antibody-Western-Blot-NBP1-49921-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CACYBP Antibody [NBP1-49921] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody NBP1-49921 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody NBP1-49922, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![Immunocytochemistry/Immunofluorescence: CACYBP Antibody [NBP2-55091] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/CACYBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55091-img0001.jpg "Immunocytochemistry/Immunofluorescence: CACYBP Antibody [NBP2-55091] - Staining of human cell line U-2 OS shows localization to cytosol.")
![ELISA: Mouse CACYBP ELISA Kit (Colorimetric) [NBP2-74963] - Samples were spiked with high concentrations of Mouse CACYBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-74963_mouse-cacybp-elisa-kit-colorimetric-13220241601219.jpg "ELISA: Mouse CACYBP ELISA Kit (Colorimetric) [NBP2-74963] - Samples were spiked with high concentrations of Mouse CACYBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse CACYBP ELISA Kit (Colorimetric) [NBP2-74963] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-74963_mouse-cacybp-elisa-kit-colorimetric-432024913321.jpg)
![ELISA: Human CACYBP ELISA Kit (Colorimetric) [NBP2-74962] - Samples were spiked with high concentrations of Human CACYBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-74962_human-cacybp-elisa-kit-colorimetric-132202416254074.jpg "ELISA: Human CACYBP ELISA Kit (Colorimetric) [NBP2-74962] - Samples were spiked with high concentrations of Human CACYBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CACYBP ELISA Kit (Colorimetric) [NBP2-74962] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-74962_human-cacybp-elisa-kit-colorimetric-432024983520.jpg)
![Western Blot: CACYBP Overexpression Lysate (Adult Normal) [NBL1-08636] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CACYBP.](http://images.novusbio.com/fullsize/CACYBP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08636-img0002.jpg "Western Blot: CACYBP Overexpression Lysate (Adult Normal) [NBL1-08636] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CACYBP.")
![Western Blot: CACYBP Overexpression Lysate (Adult Normal) [NBL1-08637] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CACYBP.](http://images.novusbio.com/fullsize/CACYBP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08637-img0002.jpg "Western Blot: CACYBP Overexpression Lysate (Adult Normal) [NBL1-08637] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CACYBP.")