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![Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of Bax, mean +/- SD is 1.006 +/- 0.321 in Sham group, 37.47 +/- 10.86 in SAH + Vehicle group, 23.83 +/- 8.143 in SAH + rOPN group, F = 33.13, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean +/- SD. *P < .05, ***P < .001 vs Sham group; #P < .05, ##P < .01 vs SAH + Vehicle group Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/cns.13199) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bax-Antibody-6A7-Western-Blot-NBP1-28566-img0007.jpg "Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of Bax, mean +/- SD is 1.006 +/- 0.321 in Sham group, 37.47 +/- 10.86 in SAH + Vehicle group, 23.83 +/- 8.143 in SAH + rOPN group, F = 33.13, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean +/- SD. *P < .05, ***P < .001 vs Sham group; #P < .05, ##P < .01 vs SAH + Vehicle group Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/cns.13199) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment. IHC-F image submitted by a verified customer review](http://images.novusbio.com/fullsize/Bax-Antibody-6A7-Immunohistochemistry-Frozen-NBP1-28566-img0002.jpg)
![Western Blot: Bax Antibody (6A7) [NBP1-28566] - Analysis of lysates from human metastatic pancreatic cancer cell line L3.6pl using Bax Antibody (clone 6A7; Lot # B168-P780E). This image was submitted via reviews by a verified end user.](http://images.novusbio.com/fullsize/Bax-Antibody-6A7-Western-Blot-NBP1-28566-img0005.jpg)
![Western Blot: Bax Antibody (6A7) [NBP1-28566] - Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probled with Mouse Anti-Bax UNLB.](http://images.novusbio.com/fullsize/Bax-Antibody-6A7-Western-Blot-NBP1-28566-img0006.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0006.jpg "Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56095] - Formalin-fixed, paraffin-embedded sections of human colon cancer fom a tissue microarray stained for BAX using NB100-56095 at 1:2000. A. High Bax expression. B. Low Bax expression. Hematoxylin-eosin counterstain](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56095-img0007.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Analysis of BAX in colon tumor cell lines using this antibody. Bax is detected in the SW620 and DLD-1 cell lines. However, Bax was not detected in the cell lines (LoVo, DU145, and LS180) known to have frameshift mutations in Bax (Rampino et al. 1997). An antibody to F1ATPase was used as a protein loading control.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0004.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Analysis of Bax in Daoy whole cell lysate using anti-Bax antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0005.jpg)
![Western Blot: Bax Antibody [NB100-56096] - Analysis of Bax in mouse using this antibody. 1. In vitro translated mouse Bax. 2. Normal cortex. 3. Ischemic hippocampus. 4. Ischemic hippocampus. Ishemic samples were prepared 6 hr post middle cerebral artery occlusion. The results show the Bax expression increased following ischemia.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56096-img0003.jpg "Western Blot: Bax Antibody [NB100-56096] - Analysis of Bax in mouse using this antibody. 1. In vitro translated mouse Bax. 2. Normal cortex. 3. Ischemic hippocampus. 4. Ischemic hippocampus. Ishemic samples were prepared 6 hr post middle cerebral artery occlusion. The results show the Bax expression increased following ischemia.")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56096] - Mouse tissue sections stained for Bax using NB100-56096 at 1:2000. A. Pancreas. B. Brain showing expression of Bax in the Purkinge cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56096-img0002.jpg)
![Western Blot: Bax Antibody [NBP1-88682] - HNHA induced G0/G1 phase arrest and apoptosis in RCC cells. Western blotting showed that HNHA potently induced the expression of cell cycle arrest proteins (p53 and p21), reduced positive regulators of cell cycle progression (CDK4/6 and cyclin D1) and resulted in increased levels of anti-apoptotic (Bax and Apaf-1) proteins in RCC cells. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-014-1003-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NBP1-88682-img0022.jpg "<b>Biological Strategies Validation. </b>Western Blot: Bax Antibody [NBP1-88682] - HNHA induced G0/G1 phase arrest and apoptosis in RCC cells. Western blotting showed that HNHA potently induced the expression of cell cycle arrest proteins (p53 and p21), reduced positive regulators of cell cycle progression (CDK4/6 and cyclin D1) and resulted in increased levels of anti-apoptotic (Bax and Apaf-1) proteins in RCC cells. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-014-1003-1), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human spleen shows moderate to strong cytoplasmic positivity.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0019.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0015.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0016.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP1-88682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP1-88682-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-67285-img0007.jpg "Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded mouse testis tissue using anti-Bax antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP2-67285-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-67285-img0002.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded human cervical tissue using anti-Bax antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP2-67285-img0003.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded human gall bladder tissue using anti-Bax antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP2-67285-img0004.jpg)
![Western Blot: Bax Antibody [NBP2-67285] - Analysis of BAX on different lysates with Rabbit anti-BAX antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: HEK-293 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 21 kDaObserved band size: 21 kDaExposure time: 1 minute 34 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67285_rabbit-monoclonal-bax-antibody-western-blot-1872024115652..jpg)
![Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Analysis of SHSY5Y cells labeling BAX with Rabbit anti-BAX antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-BAX antibody at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.Beta Ⅲ tubulin ( red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67285_rabbit-monoclonal-bax-antibody-immunocytochemistry-immunofluorescence-1872024115923..jpg)
![Western Blot: Bax Antibody [NB100-56097] - Analysis of full-length recombinant mouse Bax using this antibody.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56097-img0003.jpg "Western Blot: Bax Antibody [NB100-56097] - Analysis of full-length recombinant mouse Bax using this antibody.")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56097] - FFPE section of dog ischemic brain cortex stained for Bax expression using this antibody at 1:2000. At 2 hr post ischemia, Bax staining was seen in the dying neuron that had morphological features of apoptosis. In contrast, mophologically normal appearing neurons lacked Bax staining (arrowheads). Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56097-img0004.jpg)
![ELISA Bax-alpha [Biotin]](http://images.novusbio.com/fullsize2/dyc820-2_human-total-bax-alpha-duoset-ic-elisa-5-plate-elisa-1692020135023.jpg "ELISA Bax-alpha [Biotin]")
![Immunocytochemistry/Immunofluorescence: Bax Antibody (SPM336) [NBP2-32809] - Immunofluorescence Analysis of Jurkat cells labeling BAX with Bax Antibody (SPM336) followed by Goat anti-Mouse IgG-CF488 (Green).The nuclear counterstain is NucSpot .](http://images.novusbio.com/fullsize/Bax-Antibody-SPM336-Immunocytochemistry-Immunofluorescence-NBP2-32809-img0003.jpg "Immunocytochemistry/Immunofluorescence: Bax Antibody (SPM336) [NBP2-32809] - Immunofluorescence Analysis of Jurkat cells labeling BAX with Bax Antibody (SPM336) followed by Goat anti-Mouse IgG-CF488 (Green).The nuclear counterstain is NucSpot .")
![Immunohistochemistry-Paraffin: Bax Antibody (SPM336) [NBP2-32809] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Bax Antibody (SPM336).](http://images.novusbio.com/fullsize/Bax-Antibody-SPM336-Immunohistochemistry-Paraffin-NBP2-32809-img0004.jpg)
![Flow Cytometry: Bax Antibody (SPM336) [NBP2-32809] - Flow Cytometric Analysis of human Jurkat cells using Bax Antibody (SPM336).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Bax-Antibody-SPM336-Flow-Cytometry-NBP2-32809-img0002.jpg)
![SDS-Page: Recombinant Human Bax Protein [H00000581-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Bax-Protein-SDS-Page-H00000581-P01-img0002.jpg "SDS-Page: Recombinant Human Bax Protein [H00000581-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Bax Antibody (HL236) [NBP3-13676] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/Bax-Antibody-HL236-Western-Blot-NBP3-13676-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Bax Antibody (HL236) [NBP3-13676] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676] - Bax antibody [HL236] detects Bax protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Bax stained by Bax antibody [HL236] (NBP3-13676) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Bax-Antibody-HL236-Immunohistochemistry-Paraffin-NBP3-13676-img0001.jpg)
![Western Blot: Bax Antibody (HL236) [NBP3-13676] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Bax-Antibody-HL236-Western-Blot-NBP3-13676-img0002.jpg)
![Western Blot: Bax Antibody (HL236) [NBP3-13676] - Raw264.7 whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13676_rabbit-bax-mab-hl236-91020242041220.jpg)
![Western Blot: Bax Antibody (HL236) [NBP3-13676] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13676_rabbit-bax-mab-hl236-910202419593510.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676] - Bax antibody [HL236] detects Bax protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Bax stained by Bax antibody [HL236] (NBP3-13676) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13676_rabbit-bax-mab-hl236-9102024207599.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676] - Bax antibody [HL236] detects Bax protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Bax stained by Bax antibody [HL236] (NBP3-13676) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13676_rabbit-bax-mab-hl236-910202420134092.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Bax Antibody (HL236) [NBP3-13676] - Wild-type (WT) and Bax knockout (KO) Expi293 cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13676_rabbit-bax-mab-hl236-1610202419405715.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Bax Antibody (HL236) [NBP3-13676] - Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13676_rabbit-bax-mab-hl236-1610202419414535.jpg)