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![Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38693] - Analysis in human testis and pancreas tissues using NBP2-38693 antibody. Corresponding BAG6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-Paraffin-NBP2-38693-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38693] - Analysis in human testis and pancreas tissues using NBP2-38693 antibody. Corresponding BAG6 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BAT3/BAG6 Antibody [NBP2-38693] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38693-img0004.jpg)
![Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38693] - Staining of human testis shows strong nuclear and cytoplasmic positivity in spermatogonia.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-Paraffin-NBP2-38693-img0013.jpg)
![Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38693] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-Paraffin-NBP2-38693-img0010.jpg)
![Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38693] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-Paraffin-NBP2-38693-img0011.jpg)
![Western Blot: BAT3/BAG6 Antibody [NBP1-18928] - Detection of Human BAT3 by Western Blot. Samples: Whole cell lysate (50, 5 and 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Western-Blot-NBP1-18928-img0003.jpg "Western Blot: BAT3/BAG6 Antibody [NBP1-18928] - Detection of Human BAT3 by Western Blot. Samples: Whole cell lysate (50, 5 and 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Western Blot: BAT3/BAG6 Antibody [NBP1-18928] - Whole cell lysate from HeLa cells. BAT3 was also immunoprecipitated by rabbit anti-BAT3 antibody NBP1-18929.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Western-Blot-NBP1-18928-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18928] - Detection of human BAT3 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 used for IP at 6 ug per reaction. BAT3 was also immunoprecipitated by rabbit anti-BAT3 antibody NBP1-18927. For blotting immunoprecipitated BAT3, NBP1-18928 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunoprecipitation-NBP1-18928-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18928] - Detection of human BAT3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAT3 antibody NBP1-18928 (lot NBP1-18928-2) used for IP at 6 ug per reaction. BAT3 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-18928-1) and rabbit anti-BAT3 antibody NBP1-18927. For blotting immunoprecipitated BAT3, NBP1-18928 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunoprecipitation-NBP1-18928-img0005.jpg)
![Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38537] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-Paraffin-NBP2-38537-img0003.jpg "Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38537] - Staining of human testis shows high expression.")
![Immunohistochemistry: BAT3/BAG6 Antibody [NBP2-38537] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-NBP2-38537-img0001.jpg)
![Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38537] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-Paraffin-NBP2-38537-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP2-38537] - Staining in human testis and liver tissues using anti-BAG6 antibody. Corresponding BAG6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-Paraffin-NBP2-38537-img0004.jpg)
![Western Blot: BAT3/BAG6 Antibody (SR1223) [NBP3-22332] - Western blot analysis using NBP3-22332 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22332_rabbit-bat3-bag6-mab-sr1223-57202316112174.jpg "Western Blot: BAT3/BAG6 Antibody (SR1223) [NBP3-22332] - Western blot analysis using NBP3-22332 in A431 cell lysate.")
![Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP1-36941] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunohistochemistry-Paraffin-NBP1-36941-img0001.jpg "Immunohistochemistry-Paraffin: BAT3/BAG6 Antibody [NBP1-36941] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: BAT3/BAG6 Antibody [NBP2-15547] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Western-Blot-NBP2-15547-img0006.jpg "Western Blot: BAT3/BAG6 Antibody [NBP2-15547] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: BAT3/BAG6 Antibody [NBP2-15547] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BAT3 protein stained by BAT3 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15547-img0005.jpg)
![Western Blot: BAT3/BAG6 Antibody [NBP1-18927] - Detection of human BAT3 by western blot. Samples: Nuclear extract from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-BAT3 antibody NBP1-18927 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Western-Blot-NBP1-18927-img0004.jpg "Western Blot: BAT3/BAG6 Antibody [NBP1-18927] - Detection of human BAT3 by western blot. Samples: Nuclear extract from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-BAT3 antibody NBP1-18927 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18927] - Detection of human BAT3 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAT3 antibody NBP1-18927 used for IP at 6 ug per reaction. BAT3 was also immunoprecipitated by rabbit anti-BAT3 antibody NBP1-18928. For blotting immunoprecipitated BAT3, NBP1-18927 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-Immunoprecipitation-NBP1-18927-img0005.jpg)
![Western Blot: BAT3/BAG6 Antibody (8P4D9) [NBP3-15695] - Analysis of extracts of various cell lines, using BAT3/BAG6 antibody (NBP3-15695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/BAT3-BAG6-Antibody-8P4D9-Western-Blot-NBP3-15695-img0001.jpg "Western Blot: BAT3/BAG6 Antibody (8P4D9) [NBP3-15695] - Analysis of extracts of various cell lines, using BAT3/BAG6 antibody (NBP3-15695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
