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![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining in human cerebral cortex and liver tissues . Corresponding ATRX RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0026.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining in human cerebral cortex and liver tissues . Corresponding ATRX RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ATRX Antibody [NBP1-83077] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATRX antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/ATRX-Antibody-Western-Blot-NBP1-83077-img0028.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody [NBP1-83077] - Staining of human cell line A549 shows localization to nucleoplasm and nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83077-img0029.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0027.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons and glial cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0022.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0023.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human uterine cervix shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-83077-img0025.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human lung cancer. ATRX stained by ATRX antibody [C3], C-term diluted at 1:2500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-32851-img0028.jpg "Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human lung cancer. ATRX stained by ATRX antibody [C3], C-term diluted at 1:2500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATRX Antibody [NBP1-32851] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term.](http://images.novusbio.com/fullsize/ATRX-Antibody-Western-Blot-NBP1-32851-img0020.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human endometrial carcinoma. Rad54 antibody [C3], C-term dilution: 1:500.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-32851-img0011.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human breast carcinoma. ATRX stained by ATRX antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-32851-img0025.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-32851] - Human lung cancer. ATRX stained by ATRX antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunohistochemistry-Paraffin-NBP1-32851-img0030.jpg)
![Immunoprecipitation: ATRX Antibody [NBP1-32851] - Immunoprecipitation of ATRX protein from 293T whole cell extracts using 5 ug of ATRX antibody [C3], C-term (NBP1-32851). Western blot analysis was performed using ATRX antibody [C3], C-term (NBP1-32851). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunoprecipitation-NBP1-32851-img0016.jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32851_rabbit-polyclonal-atrx-antibody-western-blot-104202491732..jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32851_rabbit-polyclonal-atrx-antibody-western-blot-104202492044..jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32851_rabbit-polyclonal-atrx-antibody-western-blot-104202492449..jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - mRNA expression & protein complex formation of various ChRFs in the brain & in embryonic stem cells (ESCs). (A) Schematic of regions that were assessed for RNA levels of several ChRFs: Bs, brain stem; Ce, cerebellum; Mb, midbrain; Hth, hypothalamus; Hc, Th, S, hippocampus/thalamus/septum; Ctx, cortex; & Ob, olfactory bulb. (B) ChRFs fall into two large expression groups (I + II) in the brain. RT-qPCR results were expressed as delta CT values (reference gene: Gapdh) which were then centered at the median & subjected to hierarchical clustering. Red color indicates high expression (negative delta CT value) & green color indicates low expression (positive delta CT value). (C) Superose 6 size exclusion chromatography of ChRF peak fractions after anion exchange chromatography followed by immunoblot analysis of CHD3, CHD5, & ATRX as well as HDAC1, 2, & 3. The arrowhead indicates the void volume of the column, the asterisk marks a scanning artifact. Molecular masses of defined marker proteins are indicated at the bottom of their corresponding elution fractions. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnbeh.2015.00313/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32851_rabbit-polyclonal-atrx-antibody-31020241616093.jpg)
![Immunocytochemistry/ Immunofluorescence: ATRX Antibody [NBP1-32851] - ATRX antibody [C3], C-term detects ATRX protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATRX stained by ATRX antibody [C3], C-term (NBP1-32851) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-32851_rabbit-polyclonal-atrx-antibody-910202419593566.jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (NBP1-32851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32851_rabbit-polyclonal-atrx-antibody-91020242021954.jpg)
![Western Blot: ATRX Antibody [NBP1-32851] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [C3], C-term (NBP1-32851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32851_rabbit-polyclonal-atrx-antibody-91020242011440.jpg)
![Western Blot: ATRX Antibody (CL0537) [NBP2-52938] - analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATRX antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Western-Blot-NBP2-52938-img0023.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATRX Antibody (CL0537) [NBP2-52938] - analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATRX antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in U251 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunocytochemistry-Immunofluorescence-NBP2-52938-img0022.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunohistochemistry-Paraffin-NBP2-52938-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in A431 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunocytochemistry-Immunofluorescence-NBP2-52938-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in HeLa cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunocytochemistry-Immunofluorescence-NBP2-52938-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in MCF7 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunocytochemistry-Immunofluorescence-NBP2-52938-img0003.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunohistochemistry-Paraffin-NBP2-52938-img0016.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human glioma shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunohistochemistry-Paraffin-NBP2-52938-img0017.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/ATRX-Antibody-CL0537-Immunohistochemistry-Paraffin-NBP2-52938-img0018.jpg)
![Western Blot: ATRX Antibody (HL1210) [NBP3-13663] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL3] (NBP3-13663) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATRX-Antibody-HL1210-Western-Blot-NBP3-13663-img0001.jpg "Western Blot: ATRX Antibody (HL1210) [NBP3-13663] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL3] (NBP3-13663) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ATRX Antibody (HL1210) [NBP3-13663] - ATRX antibody [HL1210] detects ATRX protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATRX stained by ATRX antibody [HL1210] (NBP3-13663) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/ATRX-Antibody-HL1210-Immunocytochemistry-Immunofluorescence-NBP3-13663-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ATRX Antibody (HL1210) [NBP3-13663] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL1210] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13663_rabbit-atrx-mab-hl1210-1610202419405761.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATRX Antibody (HL1210) [NBP3-13663] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL1210] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13663_rabbit-atrx-mab-hl1210-1610202419405792.jpg)
![Western Blot: ATRX Antibody (4212) [NBP2-43599] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [GT4212].](http://images.novusbio.com/fullsize/ATRX-Antibody-4212-Western-Blot-NBP2-43599-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATRX Antibody (4212) [NBP2-43599] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [GT4212].")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATRX Antibody (4212) [NBP2-43599] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATRX protein stained by ATRX antibody [4212] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ATRX-Antibody-4212-Immunocytochemistry-Immunofluorescence-NBP2-43599-img0004.jpg)
![Western Blot: ATRX Antibody (4212) [NBP2-43599] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 5 % SDS-PAGE ATRX antibody [4212] dilution: 1:500.](http://images.novusbio.com/fullsize/ATRX-Antibody-4212-Western-Blot-NBP2-43599-img0001.jpg)
![Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643] - Formalin-fixed, paraffin-embedded human glioma stained with ATRX Antibody (rATRX/3446).](http://images.novusbio.com/fullsize/ATRX-Antibody-rATRX-3446-Immunohistochemistry-Paraffin-NBP3-07643-img0002.jpg "Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643] - Formalin-fixed, paraffin-embedded human glioma stained with ATRX Antibody (rATRX/3446).")
![Immunohistochemistry-Paraffin: ATRX Antibody (rATRX/3446) [NBP3-07643] - Formalin-fixed, paraffin-embedded human prostate stained with ATRX Recombinant Mouse Monoclonal Antibody (rATRX/3446).](http://images.novusbio.com/fullsize/ATRX-Antibody-rATRX-3446-Immunohistochemistry-Paraffin-NBP3-07643-img0001.jpg)
![SDS-Page: Recombinant Human ATRX Protein [H00000546-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ATRX-Protein-SDS-Page-H00000546-Q01-img0001.jpg "SDS-Page: Recombinant Human ATRX Protein [H00000546-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human ATRX Protein [H00000546-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ATRX-Protein-SDS-Page-H00000546-P01-img0001.jpg "SDS-Page: Recombinant Human ATRX Protein [H00000546-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: ATRX Antibody [NBP2-55953] - Staining of human cell line SH-SY5Y shows localization to nuclear bodies.](http://images.novusbio.com/fullsize/ATRX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55953-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATRX Antibody [NBP2-55953] - Staining of human cell line SH-SY5Y shows localization to nuclear bodies.")
