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![Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Western-Blot-NB110-60928-img0004.jpg "Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.")
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NB110-60928] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NB110-60928-img0005.jpg)
![Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Western-Blot-NB110-60928-img0003.jpg)
![Western Blot: ATG16L1 Antibody [NB110-82384] - Analysis of ATG16L1 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Western-Blot-NB110-82384-img0004.jpg "Western Blot: ATG16L1 Antibody [NB110-82384] - Analysis of ATG16L1 in HeLa whole cell extracts.")
![Western Blot: ATG16L1 Antibody [NBP2-15504] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Western-Blot-NBP2-15504-img0001.jpg "Western Blot: ATG16L1 Antibody [NBP2-15504] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15504-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG16L1 Antibody [NBP2-15504] - Wild-type (WT) and ATG16L1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG16L1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15504_rabbit-polyclonal-atg16l1-antibody-161020241940578.jpg)
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-49408] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-49408-img0003.jpg "Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-49408] - Staining of human testis shows high expression.")
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-49408] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-49408-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-49408] - Staining in human testis and liver tissues using anti-ATG16L1 antibody. Corresponding ATG16L1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-49408-img0004.jpg)
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP3-14544] - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/ATG16L1 Antibody-Immunohistochemistry-Paraffin-NBP3-14544-img0002.jpg "Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP3-14544] - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Simple Western: ATG16L1 Antibody [NBP3-14544] - ATG16L1 antibody (LS-A11370, 20 g/mL) yields a specific band on capillary Western analysis (Protein Simple, WES 12-230 kDa separation module) in 1 ng purified recombinant human ATG16L1 protein.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Simple-Western-NBP3-14544-img0001.jpg)
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP3-14543] - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/ATG16L1 Antibody-Immunohistochemistry-Paraffin-NBP3-14543-img0002.jpg "Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP3-14543] - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Simple Western: ATG16L1 Antibody [NBP3-14543] - ATG16L1 antibody (LS-A11356, 20 mg/mL) yields a specific band on capillary Western analysis (Protein Simple, WES 12-230 kDa separation module) in 10 ng purified recombinant human ATG16L1 protein.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Simple-Western-NBP3-14543-img0001.jpg)
![Western Blot: ATG16L1 Antibody (S03-4G8) [NBP3-19576] - Western blot detection of ATG16L1 in 3T3 cell lysates using NBP3-19576 (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-S03-4G8-Western-Blot-NBP3-19576-img0001.jpg "Western Blot: ATG16L1 Antibody (S03-4G8) [NBP3-19576] - Western blot detection of ATG16L1 in 3T3 cell lysates using NBP3-19576 (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.")
![Western Blot: ATG16L1 Antibody (5N2N9) [NBP3-16236] - Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit mAb (NBP3-16236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-5N2N9-Western-Blot-NBP3-16236-img0001.jpg "Western Blot: ATG16L1 Antibody (5N2N9) [NBP3-16236] - Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit mAb (NBP3-16236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: ATG16L1 Overexpression Lysate (Adult Normal) [NBP2-06180] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG16L1.](http://images.novusbio.com/fullsize/ATG16L1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06180-img0001.jpg "Western Blot: ATG16L1 Overexpression Lysate (Adult Normal) [NBP2-06180] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG16L1.")