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![Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction. Evaluation of ASXL1 protein expression by Western blotting. Left-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), and KBM5 clones (labeled 1-5) with heterozygous precise correction of the ASXL1 mutation. Right-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, and KBM5 clones (labeled 1-5) with homozygous precise correction of the ASXL1 mutation. Beta-actin was used as loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/6392) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ASXL1-Antibody-6E2-Western-Blot-H00171023-M05-img0006.jpg "Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction. Evaluation of ASXL1 protein expression by Western blotting. Left-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), and KBM5 clones (labeled 1-5) with heterozygous precise correction of the ASXL1 mutation. Right-hand side: ASXL1 protein expression in the SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, and KBM5 clones (labeled 1-5) with homozygous precise correction of the ASXL1 mutation. Beta-actin was used as loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/6392) licensed under a CC-BY license.")
![Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Analysis of ASXL1 expression in MCF-7 (Cat # L046V1).](http://images.novusbio.com/fullsize/ASXL1-Antibody-6E2-Western-Blot-H00171023-M05-img0004.jpg)
![ELISA: ASXL1 Antibody (6E2) [H00171023-M05] - Detection limit for recombinant GST tagged ASXL1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ASXL1-Antibody-6E2-ELISA-H00171023-M05-img0005.jpg)
![Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction(A) Evaluation of ASXL1 protein expression by WB. Left: ASXL1 protein expression in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), & KBM5 clones (labeled 1–5) w/ heterozygous precise correction of ASXL1 mutation. Right: ASXL1 protein expression in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, & KBM5 clones (labeled 1–5) w/ homozygous precise correction of ASXL1 mutation. beta -actin used as loading control. (B) Evaluation of expression levels of HOXA genes using quantitative real-time PCR (q-RT-PCR). Expression levels of HOXA5, 6, 7, 9, 10 & 13 measured in three ASXL1 homozygous corrected KBM5 clones compared w/ uncorrected cells. Results shown obtained from six independent experiments for each clone. Values in ASXL1 homozygous corrected cells are relative to uncorrected cells. Bar graphs show mean + standard error of mean (s.e.m.) (* = P < 0.05, ** = P < 0.01, *** = P < 0.001, paired t-test). (C) Evaluation of H3K27me3 levels & expression of PRC2 components by WB in uncorrected KBM5 cells & three KBM5 clones w/ homozygous correction of ASXL1 mutation. H3K27me3 levels & total H3 levels evaluated using purified histone fractions. Expression levels of two PRC2 components (EZH2, SUZ12) determined using whole cell lysates. beta -actin used as loading control. (D) Immunoprecipitation of BAP1 in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, & three KBM5 clones w/ homozygous correction of ASXL1 mutation. The BAP1 protein fraction immunoprecipitated using a BAP1 antibody & stained for ASXL1 & BAP1. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.6392), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00171023-m05_mouse-monoclonal-asxl1-antibody-6e2-310202415485124.jpg)
![Western Blot: ASXL1 Antibody (6E2) [H00171023-M05] - Functional effects of CRISPR/Cas9-mediated ASXL1 mutation correction(A) Evaluation of ASXL1 protein expression by WB. Left: ASXL1 protein expression in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, the K562 leukemia cell line (carrying the Y591X heterozygous ASXL1 mutation), & KBM5 clones (labeled 1–5) w/ heterozygous precise correction of ASXL1 mutation. Right: ASXL1 protein expression in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, & KBM5 clones (labeled 1–5) w/ homozygous precise correction of ASXL1 mutation. beta -actin used as loading control. (B) Evaluation of expression levels of HOXA genes using quantitative real-time PCR (q-RT-PCR). Expression levels of HOXA5, 6, 7, 9, 10 & 13 measured in three ASXL1 homozygous corrected KBM5 clones compared w/ uncorrected cells. Results shown obtained from six independent experiments for each clone. Values in ASXL1 homozygous corrected cells are relative to uncorrected cells. Bar graphs show mean + standard error of mean (s.e.m.) (* = P < 0.05, ** = P < 0.01, *** = P < 0.001, paired t-test). (C) Evaluation of H3K27me3 levels & expression of PRC2 components by WB in uncorrected KBM5 cells & three KBM5 clones w/ homozygous correction of ASXL1 mutation. H3K27me3 levels & total H3 levels evaluated using purified histone fractions. Expression levels of two PRC2 components (EZH2, SUZ12) determined using whole cell lysates. beta -actin used as loading control. (D) Immunoprecipitation of BAP1 in SET2 leukemia cell line (wild-type for ASXL1), uncorrected KBM5 cells, & three KBM5 clones w/ homozygous correction of ASXL1 mutation. The BAP1 protein fraction immunoprecipitated using a BAP1 antibody & stained for ASXL1 & BAP1. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.6392), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00171023-m05_mouse-monoclonal-asxl1-antibody-6e2-310202415482292.jpg)
![Western Blot: ASXL1 Antibody [NBP2-43807] - Analysis of A. 50 ug 293T whole cell lysate/extract B. 50 ug whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells ASXL1 antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/ASXL1-Antibody-Western-Blot-NBP2-43807-img0001.jpg "Western Blot: ASXL1 Antibody [NBP2-43807] - Analysis of A. 50 ug 293T whole cell lysate/extract B. 50 ug whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells ASXL1 antibody dilution: 1:5000.")
![Immunohistochemistry-Paraffin: ASXL1 Antibody [NBP2-43807] - Human esophagus carcinoma. ASXL1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ASXL1-Antibody-Immunohistochemistry-Paraffin-NBP2-43807-img0002.jpg)
![Western Blot: ASXL1 Antibody [NBP2-43807] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ASXL1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-43807_rabbit-polyclonal-asxl1-antibody-western-blot-2820237521..jpg)
![Western Blot: ASXL1 Antibody [NBP2-92590] - Western blot analysis of extracts of Mouse liver, using ASXL1 antibody (NBP2-92590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/ASXL1 Antibody-Western Blot-NBP2-92590-img0002.jpg "Western Blot: ASXL1 Antibody [NBP2-92590] - Western blot analysis of extracts of Mouse liver, using ASXL1 antibody (NBP2-92590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: ASXL1 Overexpression Lysate (Adult Normal) [NBP2-07185] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASXL1.](http://images.novusbio.com/fullsize/ASXL1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07185-img0001.jpg "Western Blot: ASXL1 Overexpression Lysate (Adult Normal) [NBP2-07185] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASXL1.")
![Western Blot: ASXL1 Overexpression Lysate (Adult Normal) [NBP2-10136] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASXL1.](http://images.novusbio.com/fullsize/ASXL1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10136-img0001.jpg "Western Blot: ASXL1 Overexpression Lysate (Adult Normal) [NBP2-10136] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASXL1.")