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![Western Blot: Actin Gamma 1 Antibody [NB600-533] - Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. Image collected and cropped by CiteAb from the following publication (//neuraldevelopment.biomedcentral.com/articles/10.1186/s13064-016-0068-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-Western-Blot-NB600-533-img0010.jpg "Western Blot: Actin Gamma 1 Antibody [NB600-533] - Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. Image collected and cropped by CiteAb from the following publication (//neuraldevelopment.biomedcentral.com/articles/10.1186/s13064-016-0068-8), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody [NB600-533] - Detection of Actin Gamma 1 (Green) in Hela cells using NB600-533 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-533-img0003.jpg)
![Immunohistochemistry: Actin Gamma 1 Antibody [NB600-533] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-Immunohistochemistry-NB600-533-img0008.jpg)
![Western Blot: Actin Gamma 1 Antibody [NB600-533] - Whole cell lysate from HeLa (1, 2.5, 5, 15 and 50 ug) and mouse NIH3T3 cells (50 ug), probed with diluted at 0.04 ug/ml.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-Western-Blot-NB600-533-img0002.jpg)
![Western Blot: Actin Gamma 1 Antibody [NB600-533] - Actin Antibody [NB600-533]-Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-Western-Blot-NB600-533-img0004.jpg)
![Western Blot: Actin Gamma 1 Antibody [NB600-533] - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody NB600-533 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-Western-Blot-NB600-533-img0009.jpg)
![Simple Western: Actin Gamma 1 Antibody [NB600-533] - Simple Western lane view shows a specific band for Actin Gamma 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-Simple-Western-NB600-533-img0006.jpg)
![Western Blot: Actin Gamma 1 Antibody [NB600-533] - Validation of RNA-seq data by qRT-PCR & Western blot analyses. a The relative mRNA level of DLK & axon guidance genes in infected cells was analyzed by qRT-PCR, normalized to three housekeeping genes & calculated with the delta delta CT method. The value of mRNA expression for each gene in control cells (pLKO.1) was arbitrarily set to 1. Data are the mean ± SEM (error bars) from three independent experiments carried out in triplicate. *, p 0.05. b Representative Western blots showing levels of DLK, Plxna4, Nrp1 & actin in control & DLK-depleted Neuro-2a cells. c Quantitative densitometric measurements of DLK, Plxna4 & Nrp1 protein levels in infected cells. Results are normalized to actin level in control cells, which were set to 1, & represent mean ± SEM (error bars) from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27468987), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-533_rabbit-polyclonal-actin-gamma-1-antibody-31020241617354.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.](http://images.novusbio.com/fullsize/F-Actin-Antibody-NH3-Immunocytochemistry-Immunofluorescence-NB100-64792-img0001.jpg "Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792] - Human inner ear tissue section stained with Actin Gamma 1 antibody at 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-mouse at 1:200. Nuclei stained with DAPI. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-NH3-Immunohistochemistry-Paraffin-NB100-64792-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (2A3) [NBP1-97720] - Methanol fixed human dermal fibroblasts immunostained with 2A3 (1:500)](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-2A3-Immunocytochemistry-Immunofluorescence-NBP1-97720-img0003.jpg "Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (2A3) [NBP1-97720] - Methanol fixed human dermal fibroblasts immunostained with 2A3 (1:500)")
![Immunohistochemistry: Actin Gamma 1 Antibody (2A3) [NBP1-97720] - Reactivity of NBP1-97720 (up to a 1:1000 dilution) in the epithelial villi and smooth muscle cells in swine colon.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-2A3-Immunohistochemistry-NBP1-97720-img0004.jpg)
![SDS-Page: Recombinant Human Actin Gamma 1 Protein [H00000071-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Actin-Gamma-1-Protein-SDS-Page-H00000071-P01-img0001.jpg "SDS-Page: Recombinant Human Actin Gamma 1 Protein [H00000071-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Proximity Ligation Assay: Actin Gamma 1 Antibody (1G6) [H00000071-M01] - Analysis of protein-protein interactions between VASP and ACTG1. HeLa cells were stained with anti-VASP rabbit purified polyclonal 1:1200 and anti-ACTG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-1G6-Proximity-Ligation-Assay-H00000071-M01-img0001.jpg "Proximity Ligation Assay: Actin Gamma 1 Antibody (1G6) [H00000071-M01] - Analysis of protein-protein interactions between VASP and ACTG1. HeLa cells were stained with anti-VASP rabbit purified polyclonal 1:1200 and anti-ACTG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![ELISA: Actin Gamma 1 Antibody (1G6) [H00000071-M01] - Detection limit for recombinant GST tagged ACTG1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-1G6-ELISA-H00000071-M01-img0002.jpg)
![Western Blot: Actin Gamma 1 Antibody [NBP2-15258] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Antibody-Western-Blot-NBP2-15258-img0001.jpg "Western Blot: Actin Gamma 1 Antibody [NBP2-15258] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:500.")
![Western Blot: Actin Gamma 1 Antibody [NBP2-15258] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin Gamma 1 antibody (NBP2-15258) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15258_rabbit-polyclonal-actin-gamma-1-antibody-91020242091421.jpg)
![ELISA: Human Actin Gamma 1 ELISA Kit (Chemiluminescence) [NBP2-66399] - Samples were spiked with high concentrations of Human Actin Gamma 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66399_human-actin-gamma-1-elisa-kit-chemiluminescence-13220241692239.jpg "ELISA: Human Actin Gamma 1 ELISA Kit (Chemiluminescence) [NBP2-66399] - Samples were spiked with high concentrations of Human Actin Gamma 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Actin Gamma 1 ELISA Kit (Chemiluminescence) [NBP2-66399] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66399_human-actin-gamma-1-elisa-kit-chemiluminescence-432024955043.jpg)
![Western Blot: Actin Gamma 1 Overexpression Lysate (Adult Normal) [NBP2-10985] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Actin Gamma 1.](http://images.novusbio.com/fullsize/Actin-Gamma-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10985-img0001.jpg "Western Blot: Actin Gamma 1 Overexpression Lysate (Adult Normal) [NBP2-10985] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Actin Gamma 1.")