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![Immunohistochemistry: c-Abl Antibody (PSH04-08) [NBP3-31990] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-c-Abl antibody (NBP3-31990) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31990) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31990_rabbit-c-abl-mab-psh04-08-29820241435266.jpg "Immunohistochemistry: c-Abl Antibody (PSH04-08) [NBP3-31990] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-c-Abl antibody (NBP3-31990) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31990) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: c-Abl Antibody (PSH04-08) [NBP3-31990] - Immunocytochemistry analysis of K-562 cells labeling c-Abl with Rabbit anti-c-Abl antibody (NBP3-31990) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Abl antibody (NBP3-31990) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-31990_rabbit-c-abl-mab-psh04-08-2920241033409.jpg)
![Flow Cytometry: c-Abl Antibody (PSH04-08) [NBP3-31990] - Flow cytometric analysis of HeLa cells labeling c-Abl. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31990, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-31990_rabbit-c-abl-mab-psh04-08-29202416372153.jpg)
![Western Blot: c-Abl Antibody (PSH04-08) [NBP3-31990] - Western blot analysis of c-Abl on different lysates with Rabbit anti-c-Abl antibody (NBP3-31990) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: Daudi cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 123 kDa Observed band size: 130/250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31990) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31990_rabbit-c-abl-mab-psh04-08-3920248453537.jpg)
![Western Blot: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of c-Abl using c-Abl Monoclonal Antibody on HeLa Cells.](http://images.novusbio.com/fullsize/c-Abl-Antibody-8E9-Western-Blot-NBP2-49695-img0004.jpg "Western Blot: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of c-Abl using c-Abl Monoclonal Antibody on HeLa Cells.")
![Immunocytochemistry/Immunofluorescence: c-Abl Antibody (8E9) [NBP2-49695] - taining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a c-Abl monoclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.](http://images.novusbio.com/fullsize/c-Abl-Antibody-8E9-Immunocytochemistry-Immunofluorescence-NBP2-49695-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of human placenta stained with cbl antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.](http://images.novusbio.com/fullsize/c-Abl-Antibody-8E9-Immunohistochemistry-Paraffin-NBP2-49695-img0001.jpg)
![Western Blot: c-Abl Antibody [NBP2-15631] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Western-Blot-NBP2-15631-img0009.jpg "Western Blot: c-Abl Antibody [NBP2-15631] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: c-Abl Antibody [NBP2-15631] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Abl protein stained by c-Abl antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15631-img0004.jpg)
![Immunohistochemistry-Paraffin: c-Abl Antibody [NBP2-15631] - Human hepatoma, using c-Abl antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunohistochemistry-Paraffin-NBP2-15631-img0002.jpg)
![Western Blot: c-Abl Antibody [NBP2-15631] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Western-Blot-NBP2-15631-img0007.jpg)
![Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Untreated (-) and treated (+) K562 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-Abl-[p-Tyr245]-Antibody-Western-Blot-NBP3-13326-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Untreated (-) and treated (+) K562 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl (phospho Tyr245) antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-Abl-[p-Tyr245]-Antibody-Western-Blot-NBP3-13326-img0002.jpg)
![Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13326_rabbit-c-abl-p-tyr-245-pab-910202420131213.jpg)
![Western Blot: c-Abl [p Tyr245] Antibody [NBP3-13326] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Abl antibody (NBP3-13326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13326_rabbit-c-abl-p-tyr-245-pab-910202420131259.jpg)
![Immunocytochemistry/Immunofluorescence: c-Abl Antibody [NBP1-90289] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90289-img0006.jpg "Immunocytochemistry/Immunofluorescence: c-Abl Antibody [NBP1-90289] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: c-Abl Antibody [NBP1-90289] - Staining of human adrenal gland shows strong nuclear positivity in cortical cells.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunohistochemistry-Paraffin-NBP1-90289-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: c-Abl Antibody [NBP1-90287] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90287-img0004.jpg "Immunocytochemistry/Immunofluorescence: c-Abl Antibody [NBP1-90287] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.")
![Immunohistochemistry-Paraffin: c-Abl Antibody [NBP1-90287] - Staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunohistochemistry-Paraffin-NBP1-90287-img0002.jpg)
![Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23312_mouse-c-abl-mab-m209-9820231138390.jpg "Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).")
![Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23312_mouse-c-abl-mab-m209-19920231915412.jpg)
![Immunocytochemistry/Immunofluorescence: c-Abl Antibody [NBP2-99737] - Immunofluorescence staining of c-Abl in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human c-Abl polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99737-img0001.jpg "Immunocytochemistry/Immunofluorescence: c-Abl Antibody [NBP2-99737] - Immunofluorescence staining of c-Abl in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human c-Abl polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.")
![Immunohistochemistry-Paraffin: c-Abl Antibody [NBP2-99737] - Immunochemical staining of human c-Abl in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunohistochemistry-Paraffin-NBP2-99737-img0002.jpg)
![Immunohistochemistry-Paraffin: c-Abl Antibody [NBP1-90288] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/c-Abl-Antibody-Immunohistochemistry-NBP1-90288-img0004.jpg "Immunohistochemistry-Paraffin: c-Abl Antibody [NBP1-90288] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.")
![Western Blot: c-Abl [p Tyr412] Antibody [NBP3-23318] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23318_rabbit-c-abl-p-tyr412-pab-19920231915414.jpg "Western Blot: c-Abl [p Tyr412] Antibody [NBP3-23318] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).")
![Western Blot: c-Abl [p Tyr245] Antibody [NBP3-23313] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23313_rabbit-c-abl-p-tyr245-pab-199202312523642.jpg "Western Blot: c-Abl [p Tyr245] Antibody [NBP3-23313] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).")