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![Western Blot: Tight Junction Protein 2 Antibody [NBP1-86850] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-TJP2 antibody. Corresponding TJP2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Western-Blot-NBP1-86850-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Tight Junction Protein 2 Antibody [NBP1-86850] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-TJP2 antibody. Corresponding TJP2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850] - Green: CD31 Red: Tight Junction Protein 2 Blue: Nuclei. Lung frozen section was blocked and stained with TJP-2 at 1:100 dilution for a hour. A secondary Alexa 555-conjugated goat anti-rabbit antibody was used for labeling. Section was aso co-stained with CD31 antibody in conjunction with an Alexa 488-conjugated antibody. Nuclei was revealed by DAPU staining. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86850-img0009.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human pancreas shows weak cytoplasmic psotivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86850-img0022.jpg)
![Western Blot: Tight Junction Protein 2 Antibody [NBP1-86850] - Primary mouse lung endothelial cells treated with or without control IgG was blotted with the anti-TJP-2 antibody. Primary Antibody diluted 1:1000. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Western-Blot-NBP1-86850-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86850-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining in primary mouse lung endothelial cells. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86850-img0017.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human testis shows strong membranous and cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86850-img0019.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86850-img0020.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human cerebral cortex shows strong cytoplasmic psotivity in glial cells.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86850-img0021.jpg)
![Immunoprecipitation: Tight Junction Protein 2 Antibody [NBP1-86850] - HEK293 cells were lysed and immunoprecipitated wtih anti-TJP2 ab (NBP1-86850) and Protein A/G PLUS-Agarose. The precipitates were separated by SDS-PAGE and blotted with anti-ZO1 antibody. IP image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunoprecipitation-NBP1-86850-img0023.jpg)
![Western Blot: Tight Junction Protein 2 Antibody [NBP2-68905] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Western-Blot-NBP2-68905-img0001.jpg "Western Blot: Tight Junction Protein 2 Antibody [NBP2-68905] - Analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP2-68905] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cell junctions.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68905-img0002.jpg)
![Western Blot: Tight Junction Protein 2 Antibody (4D12) [H00009414-M01-100ug] - Detection against Immunogen (36.52 KDa) .](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-4D12-Western-Blot-H00009414-M01-100ug-img0003.jpg "Western Blot: Tight Junction Protein 2 Antibody (4D12) [H00009414-M01-100ug] - Detection against Immunogen (36.52 KDa) .")
![Western Blot: Tight Junction Protein 2 Antibody (4D12) [H00009414-M01-100ug] - Analysis of TJP2 expression in HepG2.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-4D12-Western-Blot-H00009414-M01-100ug-img0002.jpg)
![ELISA: Tight Junction Protein 2 Antibody (4D12) [H00009414-M01-100ug] - Detection limit for recombinant GST tagged TJP2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-4D12-ELISA-H00009414-M01-100ug-img0001.jpg)
![Western Blot: Tight Junction Protein 2 Antibody [NBP3-35078] - Western blot analysis of lysates from HeLa cells, using Tight Junction Protein 2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35078_rabbit-tight-junction-protein-2-pab-101220241252855.jpg "Western Blot: Tight Junction Protein 2 Antibody [NBP3-35078] - Western blot analysis of lysates from HeLa cells, using Tight Junction Protein 2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.")
![Immunoprecipitation: Tight Junction Protein 2 Antibody [NBP3-35078] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Tight Junction Protein 2 antibody. Western blot was performed from the immunoprecipitate using Tight Junction Protein 2 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35078_rabbit-tight-junction-protein-2-pab-10122024124399.jpg)
![SDS-Page: Recombinant Human Tight Junction Protein 2 Protein [H00009414-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00009414-Q01-1.jpg "SDS-Page: Recombinant Human Tight Junction Protein 2 Protein [H00009414-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Tight Junction Protein 2 Antibody [NBP2-20641] - Tight junction protein 2 Antibody [NBP2-20641] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-2-Antibody-Western-Blot-NBP2-20641-img0002.jpg "Western Blot: Tight Junction Protein 2 Antibody [NBP2-20641] - Tight junction protein 2 Antibody [NBP2-20641] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:500.")
![Western Blot:[NBP3-30020]-Detection of human ZO-2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T and Jurkat cells. Antibodies: Affinity purified rabbit anti-ZO-2 antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. ZO-2 was also immunoprecipitated by rabbit anti-ZO-2 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30020_rabbit-tight-junction-protein-2-pab-231202511585517.jpg "Western Blot:[NBP3-30020]-Detection of human ZO-2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T and Jurkat cells. Antibodies: Affinity purified rabbit anti-ZO-2 antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. ZO-2 was also immunoprecipitated by rabbit anti-ZO-2 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.")
![Western Blot: Tight junction protein ZO-2 Overexpression Lysate (Adult Normal) [NBL1-16938] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tight junction protein ZO-2.](http://images.novusbio.com/fullsize/Tight-junction-protein-ZO-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16938-img0002.jpg "Western Blot: Tight junction protein ZO-2 Overexpression Lysate (Adult Normal) [NBL1-16938] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tight junction protein ZO-2.")
![Immunoprecipitation:Tight Junction Protein 2 AntibodyNBP3-30162] -Detection of human ZO-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZO-2 antibody NBP3-30162 used for IP at 6 µg/mg lysate. ZO-2 was also immunoprecipitated by rabbit anti-ZO-2., which recognizes a downstream epitope. For blotting immunoprecipitated ZO-2, A303-751A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30162_rabbit-tight-junction-protein-2-pab-301220241727483.jpg "Immunoprecipitation:Tight Junction Protein 2 AntibodyNBP3-30162] -Detection of human ZO-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZO-2 antibody NBP3-30162 used for IP at 6 µg/mg lysate. ZO-2 was also immunoprecipitated by rabbit anti-ZO-2., which recognizes a downstream epitope. For blotting immunoprecipitated ZO-2, A303-751A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![ELISA: Human Tight Junction Protein 2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40251] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40251_human-tight-junction-protein-2-ready-to-use-elisa-kit-2110202415362324.png "ELISA: Human Tight Junction Protein 2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40251] - Standard Curve Reference")