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![Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-HS23-Western-Blot-NB100-407-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).")
![<b>Biological Strategies Validation. </b>Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - Analysis of HeLa (A), A431 (B), NIH3T3 (C) and PC-12 (D) cell lysate using 14-3-3 gamma antibody at 1:500.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-HS23-Western-Blot-NB100-407-img0008.jpg)
![Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Western-Blot-NB100-406-img0005.jpg "Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Immunocytochemistry-Immunofluorescence-NB100-406-img0004.jpg)
![Western Blot: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Western-Blot-NB100-406-img0002.jpg)
![Simple Western: 14-3-3 gamma [ac Val2] Antibody (KC21) [NB100-406] - Lane view shows a specific band for 14-3-3 Gamma in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-gamma-ac-Val2-Antibody-KC21-Simple-Western-NB100-406-img0007.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in NIH/3T3.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0011.jpg "Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in NIH/3T3.")
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in PC-12.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0008.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in IMR-32.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0009.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in Raw 264.7.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0010.jpg)
![Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10 Analysis of YWHAG expression in K-562.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Western-Blot-H00007532-M02-img0012.jpg)
![ELISA: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - Detection limit for recombinant GST tagged YWHAG is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-6A10-Sandwich-ELISA-H00007532-M02-img0007.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0003.jpg "Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Immunocytochemistry-Immunofluorescence-NBP2-50579-img0004.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0001.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. Recombinant Human YWHAZ Lane 2. Recombinant Human YWHAB Lane 3. Recombinant Human YWHAE Lane 4. Recombinant Human YWHAH Lane 5.: Recombinant Human YWHAG Lane 6.: Recombinant Human SFN Lane 7.: Recombinant Human YWHAQ.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0002.jpg)
![Western Blot: 14-3-3 gamma Antibody [NBP2-54679] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-Western-Blot-NBP2-54679-img0003.jpg "Western Blot: 14-3-3 gamma Antibody [NBP2-54679] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: 14-3-3 gamma Antibody [NBP2-54679] - Staining of human cerebral cortex shows cytoplasmic and nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-54679-img0005.jpg)
![Western Blot: 14-3-3 gamma Antibody [NBP2-54679] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-Western-Blot-NBP2-54679-img0002.jpg)
![Western Blot: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Western blot analysis of extracts of various cell lines, using 14-3-3 gamma Rabbit mAb (NBP3-16771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-5R4Q4-Western-Blot-NBP3-16771-img0002.jpg "Western Blot: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Western blot analysis of extracts of various cell lines, using 14-3-3 gamma Rabbit mAb (NBP3-16771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: 14-3-3 gamma Antibody (5R4Q4) [NBP3-16771] - Immunohistochemistry of paraffin-embedded mouse brain using 14-3-3 gamma Rabbit mAb (NBP3-16771) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-5R4Q4-Immunohistochemistry-Paraffin-NBP3-16771-img0001.jpg)
![Immunohistochemistry: 14-3-3 gamma Antibody (13C9) [NBP3-26246] - Image of 14-3-3 gamma Antibody (13C9) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26246_rabbit-14-3-3-gamma-mab-13c9-262202415355723.jpg "Immunohistochemistry: 14-3-3 gamma Antibody (13C9) [NBP3-26246] - Image of 14-3-3 gamma Antibody (13C9) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: 14-3-3 gamma Antibody (13C9) [NBP3-26246] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, K562 whole cell lysate. All lanes: 14-3-3 gamma Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29 kDa Observed band size: 29 kDa](http://images.novusbio.com/fullsize/nbp3-26246_rabbit-14-3-3-gamma-mab-13c9-282202411485818.jpg)
![Western Blot: 14-3-3 gamma Antibody (S01-4C7) [NBP3-20083] - Western blot detection of 14-3-3 gamma in HL-60, U251 cell lysates using NBP3-20083 (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-S01-4C7-Western-Blot-NBP3-20083-img0001.jpg "Western Blot: 14-3-3 gamma Antibody (S01-4C7) [NBP3-20083] - Western blot detection of 14-3-3 gamma in HL-60, U251 cell lysates using NBP3-20083 (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (1B4) [H00007532-M03] - Analysis of monoclonal antibody to YWHAG on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-1B4-Immunocytochemistry-Immunofluorescence-H00007532-M03-img0001.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (1B4) [H00007532-M03] - Analysis of monoclonal antibody to YWHAG on HeLa cell. Antibody concentration 10 ug/ml")
![Western Blot: 14-3-3 gamma Overexpression Lysate (Adult Normal) [NBL1-17949] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 gamma.](http://images.novusbio.com/fullsize/14-3-3-gamma-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17949-img0002.jpg "Western Blot: 14-3-3 gamma Overexpression Lysate (Adult Normal) [NBL1-17949] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 gamma.")
![SDS-Page: Recombinant Human 14-3-3 gamma Protein [NBC1-18391] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant Human 14-3-3 gamma Protein-SDS-Page-NBC1-18391-img0002.jpg "SDS-Page: Recombinant Human 14-3-3 gamma Protein [NBC1-18391] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")