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![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-86060 (A) shows similar protein distribution across tissues to independent antibody NBP1-89457 (B).](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0018.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-86060 (A) shows similar protein distribution across tissues to independent antibody NBP1-89457 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: SP100 Antibody [NBP1-86060] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-NBP1-86060-img0006.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0021.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0019.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0020.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-86060-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunocytochemistry-Immunofluorescence-NBP3-13780-img0002.jpg "Immunocytochemistry/Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.")
![Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using SP100 antibody (PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunohistochemistry-Paraffin-NBP3-13780-img0004.jpg)
![Flow Cytometry: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Flow cytometric analysis of PFA-fixed HeLa cells. SP100 antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Flow-Cytometry-NBP3-13780-img0001.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using SP100 antibody (PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Immunohistochemistry-Paraffin-NBP3-13780-img0003.jpg)
![Protein Array: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SP100 Antibody (PCRP-SP100-1B9).](http://images.novusbio.com/fullsize/SP100-Antibody-PCRP-SP100-1B9-Protein-Array-NBP3-13780-img0006.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-89457 (A) shows similar protein distribution across tissues to independent antibody NBP1-86060 (B).](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0013.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-89457 (A) shows similar protein distribution across tissues to independent antibody NBP1-86060 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP1-89457] - Staining of human cell line A-431 shows localization to nucleus & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89457-img0004.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0016.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0014.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0015.jpg)
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP1-89457-img0017.jpg)
![Western Blot: SP100 Antibody [H00006672-D01P] - Analysis of SP100 expression in mouse kidney.](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-H00006672-D01P-img0003.jpg "Western Blot: SP100 Antibody [H00006672-D01P] - Analysis of SP100 expression in mouse kidney.")
![Western Blot: SP100 Antibody [H00006672-D01P] - Analysis of SP100 expression in transfected 293T cell line by SP100 polyclonal antibody.Lane 1: SP100 transfected lysate(53.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-H00006672-D01P-img0004.jpg)
![Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 expression in transfected 293T cell line by SP100 monoclonal antibody (M02), clone 1G6.Lane 1: SP100 transfected lysate(100.417 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SP100-Antibody-1G6-Western-Blot-H00006672-M02-img0005.jpg "Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 expression in transfected 293T cell line by SP100 monoclonal antibody (M02), clone 1G6.Lane 1: SP100 transfected lysate(100.417 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 over-expressed 293 cell line, cotransfected with SP100 Validated Chimera RNAi ( Cat # H00006672-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP100 monoclonal antibody (M02), clone 1G6 (Cat # H00006672-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/SP100-Antibody-1G6-Western-Blot-H00006672-M02-img0003.jpg)
![Sandwich ELISA: SP100 Antibody (1G6) [H00006672-M02] - Detection limit for recombinant GST tagged SP100 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SP100-Antibody-1G6-Sandwich-ELISA-H00006672-M02-img0004.jpg)
![Western Blot: SP100 Antibody [NBP3-13031] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP100 antibody (NBP3-13031) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-NBP3-13031-img0002.jpg "Western Blot: SP100 Antibody [NBP3-13031] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP100 antibody (NBP3-13031) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP3-13031] - SP100 antibody detects SP100 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP100 protein stained by SP100 antibody (NBP3-13031) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13031-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97408] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97408-img0001.jpg "Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97408] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP2-97408] - Immunochemical staining of human SP100 in human placenta with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP2-97408-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97407] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97407-img0001.jpg "Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97407] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: SP100 Antibody [NBP2-97407] - Immunochemical staining of human SP100 in human gallbladder with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SP100-Antibody-Immunohistochemistry-Paraffin-NBP2-97407-img0002.jpg)
![SDS-Page: Recombinant Human SP100 Protein [H00006672-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006672-P01-1.jpg "SDS-Page: Recombinant Human SP100 Protein [H00006672-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: SP100 Antibody [NBP3-10281] - Western blot analysis of SP100 in Mouse Heart lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/SP100-Antibody-Western-Blot-NBP3-10281-img0001.jpg "Western Blot: SP100 Antibody [NBP3-10281] - Western blot analysis of SP100 in Mouse Heart lysates. Antibody dilution at 1ug/ml")
![Western Blot: SP100 Antibody [NBP2-99036] - Anti-SP100 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99036_rabbit-polyclonal-sp100-antibody-western-blot-2182023113444..jpg "Western Blot: SP100 Antibody [NBP2-99036] - Anti-SP100 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 95 kDa")
![ELISA: SP100 Antibody (2E2) [H00006672-M03] - Detection limit for recombinant GST tagged SP100 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SP100-Antibody-2E2-ELISA-H00006672-M03-img0001.jpg "ELISA: SP100 Antibody (2E2) [H00006672-M03] - Detection limit for recombinant GST tagged SP100 is approximately 0.3ng/ml as a capture antibody.")