| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72710 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2990-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03652-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3390-SP | |||
![Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Western-Blot-NBP1-47839-img0020.jpg "Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0013.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded mouse spleen tissue using MKK4/MEK4 antibody (OTI8A8). Image from verified customer review.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0022.jpg)
![Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Flow-Cytometry-NBP1-47839-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0012.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0014.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0015.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0017.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0018.jpg)
![Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunoprecipitation-NBP1-47839-img0021.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249432880.jpg "Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249403813.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Untransfected (-) and transfected (+) HeLa whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249415916.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - SEK1 / MKK4 antibody [HL1888] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-212024941452.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249355531.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249422682.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249355552.jpg "Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-212024940882.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-212024938311.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat urinary bladder. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249413375.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249362735.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249403888.jpg)
![Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts of various cell lines, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Western-Blot-NBP3-03652-img0002.jpg "Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts of various cell lines, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Knockout-Validated-NBP3-03652-img0001.jpg)
![Western Blot: MKK4/MEK4 Antibody [NBP2-57194] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Western-Blot-NBP2-57194-img0002.jpg "Western Blot: MKK4/MEK4 Antibody [NBP2-57194] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody [NBP2-57194] - Staining of human cell line HEK 293 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57194-img0001.jpg)
![Western Blot: MKK4/MEK4 Antibody (2 E4) [NBP2-22482] - Analysis of 50ug whole cell lysate.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-2-E4-Western-Blot-NBP2-22482-img0001.jpg "Western Blot: MKK4/MEK4 Antibody (2 E4) [NBP2-22482] - Analysis of 50ug whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (2 E4) [NBP2-22482] - Analysis of MAP2K4 using anti-MAP2K4 monoclonal antibody shows specific expression in HeLa cells (shown in green) but not in negative control NRK cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing MAP2K4, at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-554 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-2-E4-Immunocytochemistry-Immunofluorescence-NBP2-22482-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody [NBP2-99331] - Immunofluorescence staining of MKK4/MEK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-HUMAN MKK4/MEK4 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99331-img0001.jpg "Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody [NBP2-99331] - Immunofluorescence staining of MKK4/MEK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-HUMAN MKK4/MEK4 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.")
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunohistochemistry-Paraffin-NBP2-99331-img0003.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunohistochemistry-Paraffin-NBP2-99331-img0002.jpg)
![Western Blot: MEK4 Overexpression Lysate (Adult Normal) [NBL1-12850] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MEK4.](http://images.novusbio.com/fullsize/MEK4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12850-img0002.jpg "Western Blot: MEK4 Overexpression Lysate (Adult Normal) [NBL1-12850] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MEK4.")