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![Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103kDa/ 103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms 70 and 40kDa.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NBP1-44092-img0003.jpg "Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103kDa/ 103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms 70 and 40kDa.")
![Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser318]-Antibody-Western-Blot-NBP2-03978-img0003.jpg "Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.")
![Western Blot: HDAC7 [p Ser448] Antibody [NBP2-03979] - Analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing Ser448 and (B) fusion protein without the phosphorylated amino acid, using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser448]-Antibody-Western-Blot-NBP2-03979-img0002.jpg "Western Blot: HDAC7 [p Ser448] Antibody [NBP2-03979] - Analysis of phosphorylated HDAC-7 in (A) recombinant fusion protein containing Ser448 and (B) fusion protein without the phosphorylated amino acid, using this antibody at 1 ug/ml.")
![Western Blot: HDAC7 [p Ser155] Antibody [NBP2-24454] - Analysis of phosphorylated HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 155and (B) fusion protein containinug an unphosphorylated serine at position 155, using NBP2-24454 at 0.1 ug/ml.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser155]-Antibody-Western-Blot-NBP2-24454-img0003.jpg "Western Blot: HDAC7 [p Ser155] Antibody [NBP2-24454] - Analysis of phosphorylated HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 155and (B) fusion protein containinug an unphosphorylated serine at position 155, using NBP2-24454 at 0.1 ug/ml.")
![Western Blot: HDAC7 Antibody [NBP2-16795] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NBP2-16795-img0001.jpg "Western Blot: HDAC7 Antibody [NBP2-16795] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HDAC7 Antibody [NBP2-16795] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16795-img0004.jpg)
![Immunohistochemistry-Paraffin: HDAC7 Antibody [NBP2-16795] - Human colon carcinoma, using HDAC7 antibody at 1:250 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunohistochemistry-Paraffin-NBP2-16795-img0002.jpg)
![Immunoprecipitation: HDAC7 Antibody [NBP2-16795] - 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HDAC7 protein by 2 ug of HDAC7 antibody 10% SDS-PAGE The immunoprecipitated HDAC7 protein was detected by HDAC7 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunoprecipitation-NBP2-16795-img0003.jpg)
![Western Blot: HDAC7 Antibody [NB100-61587] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.4 ug/ml.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NB100-61587-img0004.jpg "Western Blot: HDAC7 Antibody [NB100-61587] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.4 ug/ml.")
![Immunohistochemistry-Paraffin: HDAC7 Antibody [NB100-61587] - Human prostate carcinoma (left) and mouse colon carcinoma (right). Antibody used at a dilution of 1:5,000.](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunohistochemistry-Paraffin-NB100-61587-img0003.jpg)
![Western Blot: HDAC7 Antibody [NB100-2146] - Cell line lysates were separated on SDS-PAGE and probed with 1:500 Rabbit Anti-Histone Deacetylase 7 (HDAC7) (KG-17). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase ) and a chemiluminescent substrate.Lanes:1. HeLa nuclear2. NIH-3T3](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NB100-2146-img0002.jpg "Western Blot: HDAC7 Antibody [NB100-2146] - Cell line lysates were separated on SDS-PAGE and probed with 1:500 Rabbit Anti-Histone Deacetylase 7 (HDAC7) (KG-17). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase ) and a chemiluminescent substrate.Lanes:1. HeLa nuclear2. NIH-3T3")
![Immunoprecipitation: HDAC7 Antibody [NB100-2146] - Immunoprecipitation HDAC7 was immunoprecipitated from whole cell extract of human HEK 293T cells expressing HDAC7-FLAG using Rabbit Anti-HDAC7 (KG-17) antibody (NB100-2146). The IP samples were separated on SDS-PAGE and blotted with Rabbit Anti-FLAG antibody. The antibody was developed with Goat anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate. Lane 1: 1.8 ug Antibody immunoprecipitates HDAC7 from a RIPA extract of 180 ug of 293T cells expressing HDAC7-FLAG. Lane 2: negative control (extract without antibody).](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunoprecipitation-NB100-2146-img0001.jpg)
![Western Blot: HDAC7 Antibody (6J2N9) [NBP3-15457] - Western blot analysis of extracts of various cell lines, using HDAC7 Rabbit mAb (NBP3-15457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/HDAC7-Antibody-6J2N9-Western-Blot-NBP3-15457-img0001.jpg "Western Blot: HDAC7 Antibody (6J2N9) [NBP3-15457] - Western blot analysis of extracts of various cell lines, using HDAC7 Rabbit mAb (NBP3-15457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: HDAC7 Antibody [NBP2-98759] - Anti-HDAC7 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa](http://images.novusbio.com/fullsize/HDAC7-Antibody-Western-Blot-NBP2-98759-img0002.jpg "Western Blot: HDAC7 Antibody [NBP2-98759] - Anti-HDAC7 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa")
![Immunoprecipitation: HDAC7 Antibody [NBP2-98759] - HDAC7 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 1 ul anti-HDAC7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HDAC7 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa](http://images.novusbio.com/fullsize/HDAC7-Antibody-Immunoprecipitation-NBP2-98759-img0001.jpg)
