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![Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Abhd5-Antibody-1F3-Immunohistochemistry-Paraffin-H00051099-M01-img0012.jpg "Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.")
![Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 expression in HeLa, mouse and bovine tissues. Primary: 1:500 dilution. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Abhd5-Antibody-1F3-Western-Blot-H00051099-M01-img0018.jpg)
![Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in Jurkat(Cat # L017V1 ).](http://images.novusbio.com/fullsize/Abhd5-Antibody-1F3-Western-Blot-H00051099-M01-img0004.jpg)
![Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in human liver.](http://images.novusbio.com/fullsize/Abhd5-Antibody-1F3-Western-Blot-H00051099-M01-img0005.jpg)
![Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in HeLa.](http://images.novusbio.com/fullsize/Abhd5-Antibody-1F3-Western-Blot-H00051099-M01-img0014.jpg)
![Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in rat testis.](http://images.novusbio.com/fullsize/Abhd5-Antibody-1F3-Western-Blot-H00051099-M01-img0015.jpg)
![Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody (M01), clone 1F3.Lane 1: ABHD5 transfected lysate(39 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Abhd5-Antibody-1F3-Western-Blot-H00051099-M01-img0016.jpg)
![ELISA: Abhd5 Antibody (1F3) [H00051099-M01] - Detection limit for recombinant GST tagged ABHD5 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Abhd5-Antibody-1F3-ELISA-H00051099-M01-img0017.jpg)
![Western Blot: Abhd5 Antibody [NB110-41576] - CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NB110-41576-img0012.jpg "Western Blot: Abhd5 Antibody [NB110-41576] - CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license.")
![Western Blot: Abhd5 Antibody [NB110-41576] - Abhd5 overexpression lysates (NBL1-07199).](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NB110-41576-img0005.jpg)
![Western Blot: Abhd5 Antibody [NB110-41576] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Protein abundance of ATGL. In all figure panels data are expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blots are inset above each figure panel. GPAT, glycerol-3-phosphate acyltransferase; DGAT, diacylglycerol acyltransferase; ATGL, adipose triacylglycerol lipase; CGI-58, comparative gene identification 58; HSL, hormone sensitive lipase; AU, arbitrary units. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NB110-41576-img0009.jpg)
![Western Blot: Abhd5 Antibody [NB110-41576] - MCF7 cell lysates.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NB110-41576-img0003.jpg)
![Simple Western: Abhd5 Antibody [NB110-41576] - Image shows a specific band for Abhd5 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Simple-Western-NB110-41576-img0008.jpg)
![Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - A. ATGL (n = 8) & CGI-58 (n = 7) protein content in the three skeletal muscles (SOL, RG, & WG & heart).Ponceau stain for total protein was used as a loading control. Data are reported as mean±SE & bars with the same letter are not significantly different within the skeletal muscles (p<0.05). Insets: representative blots: lane 1, WG (white gastrocnemius); lane 2, RG (red gastrocnemius); lane 3, SOL (soleus). B. ATGL & CGI-58 western blot protein bands compared to representative Ponceau bands as equal loading control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41576_rabbit-polyclonal-abhd5-antibody-310202415291939.jpg)
![Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - The turnover of DAG & TAG is increased in the DGAT1 overexpressing TA muscle.(A) Western blotting of ATGL, (B) CGI58 (C) & ADRP in rat TA muscle. Data are expressed as mean ± SEM (n = 10–12). # P<0.05 HFD-DGAT1 vs. HFD-control. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41576_rabbit-polyclonal-abhd5-antibody-310202415171314.jpg)
![Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Effects of treadmill running on the mRNA & protein expressions of ATGL (A,E), CGI-58 (B,F), G0S2 (C,G), HSL (D,H) in the right ventricle. Data are expressed as mean ± SEM. For the sake of clarity, the control group was set at 100, & exercised groups were scaled with respect to Ctrl * p < 0.05 difference vs. control (Ctrl); #p < 0.05 difference M120 vs. M30; $p < 0.05 difference F30 vs. M30. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31137663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41576_rabbit-polyclonal-abhd5-antibody-31020241539733.jpg)
![Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Protein content of important players involved in lipid droplet dynamics in Caucasian & South Asian subjects before & after a 5-day HFHC-diet.(a) ATGL, (b) CGI-58, (c) PLIN2, (d) PLIN3 & (e) PLIN5 protein content. Data are presented as mean ± SEM & were statistically analyzed with a Repeated Measures ANOVA; *P < 0.05 for diet effect, #P < 0.05 for group effect. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep42393), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41576_rabbit-polyclonal-abhd5-antibody-3102024153973.jpg)
![Western Blot: Abhd5 Antibody - BSA Free [NB110-41576] - Effects of treadmill running on the mRNA & protein expressions of ATGL (A,E), CGI-58 (B,F), G0S2 (C,G), HSL (D,H) in the left ventricle. Data are expressed as mean ± SEM. For the sake of clarity, the control group was set at 100, & exercised groups were scaled with respect to Ctrl * p < 0.05 difference vs. control (Ctrl); #p < 0.05 difference M120 vs. M30; $p < 0.05 difference F30 vs. M30. Adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), G0/G1 switch gene 2 (G0S2), hormone sensitive lipase (HSL). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31137663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41576_rabbit-polyclonal-abhd5-antibody-31020241539716.jpg)
![Western Blot: Abhd5 Antibody [NB100-57850] - Mesenchymal stem cells differentiated into adipocytes. Antibody at 1:1000 in 5% BSA. Clear, strong band at expected molecular weight. Image from verified customer review.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NB100-57850-img0008.jpg "Western Blot: Abhd5 Antibody [NB100-57850] - Mesenchymal stem cells differentiated into adipocytes. Antibody at 1:1000 in 5% BSA. Clear, strong band at expected molecular weight. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Abhd5 Antibody [NB100-57850] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and vesicle/ cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57850-img0006.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NB100-57850-img0002.jpg)
![Western Blot: Abhd5 Antibody [NB100-57850] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer), antibody at 0.2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NB100-57850-img0005.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NB100-57850-img0001.jpg)
![Western Blot: Abhd5 Antibody [NBP1-84506] - Analysis in control (vector only transfected HEK293T lysate) and ABHD5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NBP1-84506-img0007.jpg "Western Blot: Abhd5 Antibody [NBP1-84506] - Analysis in control (vector only transfected HEK293T lysate) and ABHD5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: Abhd5 Antibody [NBP1-84506] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84506-img0006.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84506] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84506-img0002.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84506] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84506_rabbit-polyclonal-abhd5-antibody-immunohistochemistry-paraffin-286202312356..jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84506] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/antibody/nbp1-84506_rabbit-polyclonal-abhd5-antibody-immunohistochemistry-paraffin-2862023123626..jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84506] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84506_rabbit-polyclonal-abhd5-antibody-immunohistochemistry-paraffin-2862023123738..jpg)
![Western Blot: Abhd5 Antibody [NBP1-84507] - Analysis in control (vector only transfected HEK293T lysate) and ABHD5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Abhd5-Antibody-Western-Blot-NBP1-84507-img0006.jpg "Western Blot: Abhd5 Antibody [NBP1-84507] - Analysis in control (vector only transfected HEK293T lysate) and ABHD5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human testis shows moderate cytoplasmic positivity in a small subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0009.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0005.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0007.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0008.jpg)
![Immunohistochemistry-Paraffin: Abhd5 Antibody [NBP1-84507] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Abhd5-Antibody-Immunohistochemistry-Paraffin-NBP1-84507-img0010.jpg)
![Flow Cytometry: Abhd5 Antibody (JE60-62) [NBP3-31989] - Flow cytometric analysis of NIH/3T3 cells labeling Abhd5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31989, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-31989_rabbit-abhd5-mab-je60-62-29202416453253.jpg "Flow Cytometry: Abhd5 Antibody (JE60-62) [NBP3-31989] - Flow cytometric analysis of NIH/3T3 cells labeling Abhd5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31989, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
![Western Blot: Abhd5 Antibody (JE60-62) [NBP3-31989] - Western blot analysis of Abhd5 on different lysates with Rabbit anti-Abhd5 antibody (NBP3-31989) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 ug/Lane) Lane 2: Mouse pancreas tissue lysate (40 ug/Lane) Lane 3: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 39 kDa Observed band size: 43 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31989_rabbit-abhd5-mab-je60-62-3920248453521.jpg)
![Western Blot: Abhd5 Antibody (SR1756) [NBP3-21931] - Western blot analysis using NBP3-21931 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21931_rabbit-abhd5-mab-sr1756-57202317215112.jpg "Western Blot: Abhd5 Antibody (SR1756) [NBP3-21931] - Western blot analysis using NBP3-21931 in HepG2 cell lysate.")
![Recombinant Human Abhd5 Protein [H00051099-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00051099-P01-1.jpg "Recombinant Human Abhd5 Protein [H00051099-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Abhd5 Antibody (2K8W9) [NBP3-16675] - Western blot analysis of extracts of various cell lines, using Abhd5 Rabbit mAb (NBP3-16675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Abhd5-Antibody-2K8W9-Western-Blot-NBP3-16675-img0001.jpg "Western Blot: Abhd5 Antibody (2K8W9) [NBP3-16675] - Western blot analysis of extracts of various cell lines, using Abhd5 Rabbit mAb (NBP3-16675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Abhd5 Overexpression Lysate (Adult Normal) [NBL1-07199] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Abhd5.](http://images.novusbio.com/fullsize/Abhd5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07199-img0002.jpg "Western Blot: Abhd5 Overexpression Lysate (Adult Normal) [NBL1-07199] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Abhd5.")