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![Western Blot: PBX1 Antibody (4A2) [H00005087-M01] - PBX1 monoclonal antibody (M01), clone 4A2 Analysis of PBX1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/PBX1-Antibody-4A2-Western-Blot-H00005087-M01-img0010.jpg "Western Blot: PBX1 Antibody (4A2) [H00005087-M01] - PBX1 monoclonal antibody (M01), clone 4A2 Analysis of PBX1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PBX1-Antibody-4A2-Immunocytochemistry-Immunofluorescence-H00005087-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PBX1-Antibody-4A2-Immunohistochemistry-Paraffin-H00005087-M01-img0008.jpg)
![Sandwich ELISA: PBX1 Antibody (4A2) [H00005087-M01] - Detection limit for recombinant GST tagged PBX1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PBX1-Antibody-4A2-Sandwich-ELISA-H00005087-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Analysis in human fallopian tube and liver tissues using NBP1-85803 antibody. Corresponding PBX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-85803-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Analysis in human fallopian tube and liver tissues using NBP1-85803 antibody. Corresponding PBX1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human endometrium, fallopian tube, liver and prostate using Anti-PBX1 antibody NBP1-85803 (A) shows similar protein distribution across tissues to independent antibody NBP1-86151 (B).](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-85803-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: PBX1 Antibody [NBP1-85803] - Analysis in human cell lines HeLa and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/PBX1-Antibody-Western-Blot-NBP1-85803-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PBX1 Antibody [NBP1-85803] - Staining of human cell line U-251MG shows positivity in nuclei but not nucleoli.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85803-img0003.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human prostate shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-85803-img0014.jpg)
![Western Blot: PBX1 Antibody [NBP1-85803] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PBX1-Antibody-Western-Blot-NBP1-85803-img0007.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-85803-img0012.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human endometrium shows moderate to strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-85803-img0013.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-85803] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-85803-img0015.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-86151] - Analysis in human fallopian tube and liver tissues using NBP1-86151 antibody. Corresponding PBX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-86151-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-86151] - Analysis in human fallopian tube and liver tissues using NBP1-86151 antibody. Corresponding PBX1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-86151] - Staining of human endometrium, fallopian tube, liver and prostate using Anti-PBX1 antibody NBP1-86151 (A) shows similar protein distribution across tissues to independent antibody NBP1-85803 (B).](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-86151-img0007.jpg)
![Western Blot: PBX1 Antibody [NBP1-86151] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/PBX1-Antibody-Western-Blot-NBP1-86151-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PBX1 Antibody [NBP1-86151] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86151-img0005.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-86151] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-86151-img0012.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-86151] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-86151-img0009.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-86151] - Staining of human endometrium shows moderate nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-86151-img0010.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody [NBP1-86151] - Staining of human prostate shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunohistochemistry-Paraffin-NBP1-86151-img0011.jpg)
![Western Blot: PBX1 Antibody (1C11) [NBP2-00872] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.](http://images.novusbio.com/fullsize/PBX1-Antibody-1C11-Western-Blot-NBP2-00872-img0002.jpg "Western Blot: PBX1 Antibody (1C11) [NBP2-00872] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.")
![Immunocytochemistry/Immunofluorescence: PBX1 Antibody (1C11) [NBP2-00872] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1.](http://images.novusbio.com/fullsize/PBX1-Antibody-1C11-Immunocytochemistry-Immunofluorescence-NBP2-00872-img0004.jpg)
![Immunohistochemistry-Paraffin: PBX1 Antibody (1C11) [NBP2-00872] - Staining of paraffin-embedded Human liver tissue using anti-PBX1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PBX1-Antibody-1C11-Immunohistochemistry-Paraffin-NBP2-00872-img0003.jpg)
![Flow Cytometry: PBX1 Antibody (1C11) [NBP2-00872] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PBX1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PBX1-Antibody-1C11-Flow-Cytometry-NBP2-00872-img0001.jpg)
![Western Blot: PBX1 Antibody [NBP2-19713] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBX1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PBX1-Antibody-Western-Blot-NBP2-19713-img0004.jpg "Western Blot: PBX1 Antibody [NBP2-19713] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBX1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PBX1 Antibody [NBP2-19713] - PBX1 antibody detects PBX1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PBX1 stained by PBX1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19713-img0005.jpg)
![Western Blot: PBX1 Antibody [NBP2-19713] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PBX1-Antibody-Western-Blot-NBP2-19713-img0001.jpg)
![Immunoprecipitation: PBX1 Antibody [NBP2-19713] - 293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of PBX1 protein by 2 ug of PBX1 antibody 12 % SDS-PAGE The immunoprecipitated PBX1 protein was detected by PBX1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunoprecipitation-NBP2-19713-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PBX1 Antibody (PCRP-PBX1-3C8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/PBX1-Antibody-PCRP-PBX1-3C8-Immunocytochemistry-Immunofluorescence-NBP3-13991-img0002.jpg "Immunocytochemistry/Immunofluorescence: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PBX1 Antibody (PCRP-PBX1-3C8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Flow Cytometry: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991] - Flow cytometric analysis of PFA-fixed HeLa cells. PBX1 antibody (PCRP-PBX1-3C8) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/PBX1-Antibody-PCRP-PBX1-3C8-Flow-Cytometry-NBP3-13991-img0001.jpg)
![Protein Array: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PBX1 Antibody (PCRP-PBX1-3C8).](http://images.novusbio.com/fullsize/PBX1-Antibody-PCRP-PBX1-3C8-Protein-Array-NBP3-13991-img0004.jpg)
![Western Blot: PBX1 Antibody (PSH0-24) [NBP3-32720] - Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (NBP3-32720) at 1/1,000 dilution. Lane 1: Hela cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: MCF-7 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Lane 8: Rat pancreas tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 40/47 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32720) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32720_rabbit-pbx1-mab-psh0-24-278202416285282.jpg "Western Blot: PBX1 Antibody (PSH0-24) [NBP3-32720] - Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (NBP3-32720) at 1/1,000 dilution. Lane 1: Hela cell lysate (20 ug/Lane) Lane 2: HEK-293 cell lysate (20 ug/Lane) Lane 3: MCF-7 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Lane 8: Rat pancreas tissue lysate (40 ug/Lane) Predicted band size: 47 kDa Observed band size: 40/47 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32720) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PBX1 Antibody (PSH0-24) [NBP3-32720] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PBX1 antibody (NBP3-32720) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32720) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32720_rabbit-pbx1-mab-psh0-24-29820241435291.jpg)
![Western Blot: PBX1 Antibody [NBP1-52042] - NBP1-52042 (0.5ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PBX1-Antibody-Western-Blot-NBP1-52042-img0002.jpg "Western Blot: PBX1 Antibody [NBP1-52042] - NBP1-52042 (0.5ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![SDS-Page: Recombinant Human PBX1 Protein [H00005087-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005087-Q01-1.jpg "SDS-Page: Recombinant Human PBX1 Protein [H00005087-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: PBX1 Antibody (3F7) [H00005087-M02] - PBX1 monoclonal antibody (M02), clone 3F7 Analysis of PBX1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/PBX1-Antibody-3F7-Western-Blot-H00005087-M02-img0004.jpg "Western Blot: PBX1 Antibody (3F7) [H00005087-M02] - PBX1 monoclonal antibody (M02), clone 3F7 Analysis of PBX1 expression in Hela S3 NE.")
![Sandwich ELISA: PBX1 Antibody (3F7) [H00005087-M02] - Detection limit for recombinant GST tagged PBX1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PBX1-Antibody-3F7-Sandwich-ELISA-H00005087-M02-img0003.jpg)
![Western Blot: PBX1 Overexpression Lysate (Adult Normal) [NBL1-14137] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PBX1.](http://images.novusbio.com/fullsize/PBX1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14137-img0002.jpg "Western Blot: PBX1 Overexpression Lysate (Adult Normal) [NBL1-14137] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PBX1.")
![Immunocytochemistry/Immunofluorescence: PBX1 Antibody [NBP2-98718] - Immunofluorescence staining of PBX1 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PBX1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/PBX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98718-img0001.jpg "Immunocytochemistry/Immunofluorescence: PBX1 Antibody [NBP2-98718] - Immunofluorescence staining of PBX1 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PBX1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.")