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![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Analysis in human skeletal muscle and liver tissues. Corresponding FLNC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Analysis in human skeletal muscle and liver tissues. Corresponding FLNC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FLNC Antibody [NBP1-89300] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining shown in green.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89300-img0009.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0015.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0006.jpg)
![Immunohistochemistry-Frozen: FLNC Antibody [NBP1-89300] - Mouse muscle vasus transverse cryosection stained with Filamin C antibody (NBP1-89300). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Frozen-NBP1-89300-img0012.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Staning of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0013.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP1-89300] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP1-89300-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: FLNC Antibody - BSA Free [NBP2-79816] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FLNC Antibody (NBP2-79816) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/FLNC-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79816-img0007.jpg "Immunocytochemistry/Immunofluorescence: FLNC Antibody - BSA Free [NBP2-79816] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FLNC Antibody (NBP2-79816) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: FLNC Antibody - BSA Free [NBP2-79816] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FLNC Antibody conjugated to Alexa Fluor 647 (NBP2-79816AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/FLNC-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79816-img0006.jpg)
![Western Blot: FLNC Antibody [NBP2-79816] - Total protein from HeLa was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-FLNC in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/FLNC-Antibody-Western-Blot-NBP2-79816-img0004.jpg)
![Immunohistochemistry-Paraffin: FLNC Antibody [NBP2-79816] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human skeletal muscle using FLNC antibody (NBP2-79816) at 1:200 dilution. The primary antibody binding to FLNC was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. Strong staining in the cytoplasm of myocytes was observed.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunohistochemistry-Paraffin-NBP2-79816-img0002.jpg)
![Flow Cytometry: FLNC Antibody - BSA Free [NBP2-79816] - An intracellular stain was performed on U2-OS cells with FLNC NBP2-79816AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/FLNC-Antibody---BSA-Free-Flow-Cytometry-NBP2-79816-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: FLNC Antibody [NBP2-79816] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FLNC at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-79816-img0001.jpg)
![Flow Cytometry: FLNC Antibody [NBP2-79816] - An intracellular stain was performed on NIH3T3 cells with NBP2-79816 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/FLNC-Antibody-Flow-Cytometry-NBP2-79816-img0005.jpg)
![Western Blot: FLNC Antibody [NBP3-04857] - Analysis of extracts of various cell lines, using FLNC antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/FLNC-Antibody-Western-Blot-NBP3-04857-img0002.jpg "Western Blot: FLNC Antibody [NBP3-04857] - Analysis of extracts of various cell lines, using FLNC antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: FLNC Antibody [NBP3-04857] - Analysis of HeLa cells using FLNC antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FLNC-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04857-img0001.jpg)
![Immunohistochemistry-Paraffin: Filamin Antibody [NBP3-17124] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Filamin-Antibody-Immunohistochemistry-Paraffin-NBP3-17124-img0002.jpg "Immunohistochemistry-Paraffin: Filamin Antibody [NBP3-17124] - Staining of human liver shows low expression as expected.")
![Immunohistochemistry-Paraffin: Filamin Antibody [NBP3-17124] - Analysis in human skeletal muscle and liver tissues using Anti-FLNC antibody. Corresponding FLNC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Filamin-Antibody-Immunohistochemistry-Paraffin-NBP3-17124-img0001.jpg)
![Immunohistochemistry-Paraffin: Filamin Antibody [NBP3-17124] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/Filamin-Antibody-Immunohistochemistry-Paraffin-NBP3-17124-img0003.jpg)
![Western Blot: Filamin Antibody [NBP2-84023] - WB Suggested Anti-FLNC Antibody. Titration: 1.0 ug/ml. Positive Control: HepG2 Whole Cell](http://images.novusbio.com/fullsize/Filamin-Antibody-Western-Blot-NBP2-84023-img0001.jpg "Western Blot: Filamin Antibody [NBP2-84023] - WB Suggested Anti-FLNC Antibody. Titration: 1.0 ug/ml. Positive Control: HepG2 Whole Cell")
![ELISA: Human FLNC - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31660] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31660_human-flnc-ready-to-use-elisa-kit-colorimetric-206202415322730.png "ELISA: Human FLNC - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31660] - Standard Curve Reference")
![ELISA: Human FLNC ELISA Kit (Colorimetric) [NBP3-31659] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31659_human-flnc-elisa-kit-colorimetric-206202415301552.png "ELISA: Human FLNC ELISA Kit (Colorimetric) [NBP3-31659] - Standard Curve Reference")