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![Western Blot: CRMP2 Antibody [NBP1-85448] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Western-Blot-NBP1-85448-img0007.jpg "Western Blot: CRMP2 Antibody [NBP1-85448] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP1-85448] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85448-img0009.jpg)
![Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-85448] - Staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-85448-img0008.jpg)
![Western Blot: CRMP2 Antibody [NBP1-85448] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Western-Blot-NBP1-85448-img0006.jpg)
![Western Blot: CRMP2 Antibody [NBP1-31762] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Western-Blot-NBP1-31762-img0008.jpg "Western Blot: CRMP2 Antibody [NBP1-31762] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP1-31762] - CRMP2 antibody [N1N3] detects CRMP2 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CRMP2 stained by CRMP2 antibody [N1N3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31762-img0006.jpg)
![Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-31762] - Rat brain. CRMP2 stained by CRMP2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-31762-img0005.jpg)
![Western Blot: CRMP2 Antibody [NBP1-31762] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Western-Blot-NBP1-31762-img0007.jpg)
![Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-31762] - DLD1 xenograft, using CRMP2 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-31762-img0002.jpg)
![Western Blot: CRMP2 Antibody [NBP2-92939] - Analysis of extracts from normal (control) and DPYSL2 knockout (KO) HeLa cells, using CRMP2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk TBST. Detection: ECL Basic Kit . Exposure time: 5s](http://images.novusbio.com/fullsize/CRMP2-Antibody-Western-Blot-NBP2-92939-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CRMP2 Antibody [NBP2-92939] - Analysis of extracts from normal (control) and DPYSL2 knockout (KO) HeLa cells, using CRMP2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk TBST. Detection: ECL Basic Kit . Exposure time: 5s")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP2-92939] - Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] CRMP2/CRMP2 Rabbit pAb (NBP2-92939) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Immunohistochemistry-Paraffin-NBP2-92939-img0004.jpg)
![Western Blot: CRMP2 Antibody [NBP2-92939] - Analysis of extracts of various cell lines, using CRMP2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Western-Blot-NBP2-92939-img0002.jpg)
![Western Blot: CRMP2 Antibody (0A1T1) [NBP3-16406] - Western blot analysis of extracts of various cell lines, using CRMP2 Rabbit mAb (NBP3-16406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/CRMP2-Antibody-0A1T1-Western-Blot-NBP3-16406-img0003.jpg "Western Blot: CRMP2 Antibody (0A1T1) [NBP3-16406] - Western blot analysis of extracts of various cell lines, using CRMP2 Rabbit mAb (NBP3-16406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry: CRMP2 Antibody (0A1T1) [NBP3-16406] - Immunofluorescence analysis of mouse brain using CRMP2 Rabbit mAb (NBP3-16406) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CRMP2-Antibody-0A1T1-Immunohistochemistry-NBP3-16406-img0002.jpg)
![Immunohistochemistry: CRMP2 Antibody (0A1T1) [NBP3-16406] - Immunofluorescence analysis of rat brain using CRMP2 Rabbit mAb (NBP3-16406) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CRMP2-Antibody-0A1T1-Immunohistochemistry-NBP3-16406-img0001.jpg)
![Western Blot: CRMP2 Antibody (SR2080) [NBP3-21788] - Western blot analysis using NBP3-21788 in (1) U87-MG cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21788_rabbit-crmp2-mab-sr2080-57202315584822.jpg "Western Blot: CRMP2 Antibody (SR2080) [NBP3-21788] - Western blot analysis using NBP3-21788 in (1) U87-MG cell lysate; (2) NIH/3T3 cell lysate.")
![Western Blot: CRMP2 Antibody (JE31-53) [NBP3-32224] - Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (10 ug/Lane) Lane 3: Neuro-2a cell lysate (15 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: Mouse brain tissue lysate (10 ug/Lane) Lane 6: Rat brain tissue lysate (10 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32224) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-278202416331210.jpg "Western Blot: CRMP2 Antibody (JE31-53) [NBP3-32224] - Western blot analysis of CRMP2 on different lysates with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (10 ug/Lane) Lane 3: Neuro-2a cell lysate (15 ug/Lane) Lane 4: PC-12 cell lysate (10 ug/Lane) Lane 5: Mouse brain tissue lysate (10 ug/Lane) Lane 6: Rat brain tissue lysate (10 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32224) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CRMP2 Antibody (JE31-53) [NBP3-32224] - Immunocytochemistry analysis of SH-SY5Y cells labeling CRMP2 with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRMP2 antibody (NBP3-32224) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-29202410484827.jpg)
![Flow Cytometry: CRMP2 Antibody (JE31-53) [NBP3-32224] - Flow cytometric analysis of SH-SY5Y cells labeling CRMP2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32224, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32224_rabbit-crmp2-mab-je31-53-29202416372179.jpg)
![Immunocytochemistry/Immunofluorescence: CRMP2 Antibody (1F11) [H00001808-M01] - Analysis of monoclonal antibody to DPYSL2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CRMP2-Antibody-1F11-Immunocytochemistry-Immunofluorescence-H00001808-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: CRMP2 Antibody (1F11) [H00001808-M01] - Analysis of monoclonal antibody to DPYSL2 on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: CRMP2 Antibody (1F11) [H00001808-M01] - Detection limit for recombinant GST tagged DPYSL2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CRMP2-Antibody-1F11-Sandwich-ELISA-H00001808-M01-img0003.jpg)
![Western Blot: CRMP2 [p Ser522] Antibody [NBP3-23262] - Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23262_rabbit-crmp2-p-ser522-pab-1992023185695.jpg "Western Blot: CRMP2 [p Ser522] Antibody [NBP3-23262] - Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: CRMP2 [p Ser522] Antibody [NBP3-23262] - Immunocytochemical labeling of CRMP2 phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-CRMP2 (Ser-522) (CP2191) antibody in the absence (Left) or presence (Right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23262_rabbit-crmp2-p-ser522-pab-199202312523616.jpg)
![Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP3-23261] - Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.](http://images.novusbio.com/fullsize/nbp3-23261_rabbit-crmp2-pab-1992023191540.jpg "Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP3-23261] - Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.")
![Western Blot: CRMP2 Antibody [NBP3-23261] - Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23261_rabbit-crmp2-pab-119202316331617.jpg)
![Western Blot: CRMP2 [p Thr555] Antibody [NBP3-23267] - Western blot of rat PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23267_rabbit-crmp2-p-thr555-pab-18920231735523.jpg "Western Blot: CRMP2 [p Thr555] Antibody [NBP3-23267] - Western blot of rat PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: CRMP2 [p Thr555] Antibody [NBP3-23267] - Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.](http://images.novusbio.com/fullsize/nbp3-23267_rabbit-crmp2-p-thr555-pab-199202312523638.jpg)
![Western Blot: CRMP2 Antibody [NBP2-97750] - Anti-CRMP2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U87MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa](http://images.novusbio.com/fullsize/CRMP2-Antibody-Western-Blot-NBP2-97750-img0004.jpg "Western Blot: CRMP2 Antibody [NBP2-97750] - Anti-CRMP2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U87MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa")
![Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP2-97750] - Immunofluorescence staining of CRMP2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CRMP2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/CRMP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97750-img0002.jpg)
![Immunoprecipitation: CRMP2 Antibody [NBP2-97750] - CRMP2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-CRMP2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CRMP2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa](http://images.novusbio.com/fullsize/CRMP2-Antibody-In-ovo-assay-NBP2-97750-img0005.jpg)
![Immunohistochemistry: CRMP2 Antibody (20D8) [NBP3-26612] - Image of CRMP2 Antibody (20D8) diluted at 1:50 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.35% DAB.](http://images.novusbio.com/fullsize/nbp3-26612_rabbit-crmp2-mab-20d8-262202414471542.jpg "Immunohistochemistry: CRMP2 Antibody (20D8) [NBP3-26612] - Image of CRMP2 Antibody (20D8) diluted at 1:50 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.35% DAB.")
![Immunocytochemistry/Immunofluorescence: CRMP2 Antibody (20D8) [NBP3-26612] - Staining of SH-SY5Y with CRMP2 Antibody (20D8) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 506-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26612_rabbit-crmp2-mab-20d8-282202412493633.jpg)
![Flow Cytometry: CRMP2 Antibody (20D8) [NBP3-26612] - Overlay Peak curve showing SH-SY5Y cells stained with CRMP2 Antibody (20D8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26612_rabbit-crmp2-mab-20d8-282202414183684.jpg)