Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is shown to positively react with mouse EDL muscle here. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0019.jpg "Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is shown to positively react with mouse EDL muscle here. This image was submitted via customer Review.")
![Immunohistochemistry: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Immunohistochemistry-NB100-1687-img0015.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Affinity purified phosphospecific antibody to monophosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19 was used at a 1:1000 dilution to detect myosin light chain by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated or insulin or CLA treated according as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gta- Rabbit IgG (611-103-122) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0014.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 l of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0016.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody: Myosin pS19/pS20 primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Regulatory Light Chain Phospho. Other band(s): None.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0017.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of phosphospecific antibody is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown. phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0018.jpg)
![Western Blot: MRCL3 [p Ser18] Antibody [NBP3-05425] - Western blot analysis of extracts from 293 cells untreated or treated with starvation using MRCL3 antibody (NBP3-05425). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/MRCL3 [p Ser18] Antibody-Western Blot-NBP3-05425-img0002.jpg "Western Blot: MRCL3 [p Ser18] Antibody [NBP3-05425] - Western blot analysis of extracts from 293 cells untreated or treated with starvation using MRCL3 antibody (NBP3-05425). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunohistochemistry-Paraffin: MRCL3 [p Ser18] Antibody [NBP3-05425] - Immunohistochemistry of paraffin-embedded human breast carcinoma using MRCL3 antibody (NBP3-05425). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser18]-Antibody-Immunohistochemistry-Paraffin-NBP3-05425-img0001.jpg)
![Western Blot: MRCL3 Antibody [NBP2-86713] - Host: Rabbit. Target Name: MYL12A. Sample Tissue: Human THP-1 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/MRCL3-Antibody-Western-Blot-NBP2-86713-img0001.jpg "Western Blot: MRCL3 Antibody [NBP2-86713] - Host: Rabbit. Target Name: MYL12A. Sample Tissue: Human THP-1 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: MRCL3 Overexpression Lysate (Adult Normal) [NBL1-13218] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MRCL3.](http://images.novusbio.com/fullsize/MRCL3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13218-img0002.jpg "Western Blot: MRCL3 Overexpression Lysate (Adult Normal) [NBL1-13218] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MRCL3.")
![Western Blot: MRCL3 Antibody [NBP2-93080] - Analysis of extracts of various cell lines, using MRCL3 .Exposure time: 90s.](http://images.novusbio.com/fullsize/MRCL3-Antibody-Western-Blot-NBP2-93080-img0001.jpg "Western Blot: MRCL3 Antibody [NBP2-93080] - Analysis of extracts of various cell lines, using MRCL3 .Exposure time: 90s.")
![SDS-Page: Recombinant Human MRCL3 Protein [NBP1-72407] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-MRCL3-Protein-SDS-Page-NBP1-72407-img0001.jpg "SDS-Page: Recombinant Human MRCL3 Protein [NBP1-72407] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")