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Ankle Stiff: Disease Bioinformatics

Research of Ankle Stiff has been linked to Joint Stiffness, Fracture, Flexed Fetal Attitude, Pain, Tibial Fractures. The study of Ankle Stiff has been mentioned in research publications which can be found using our bioinformatics tool below. Researched pathways related to Ankle Stiff include Reflex, Transport, Muscle Contraction, Wound Healing, Proprioception. These pathways complement our catalog of research reagents for the study of Ankle Stiff including antibodies and ELISA kits against SWAYING, ROCKER, AP, CP, CFD.

Top Research Reagents

We have 2666 products for the study of Ankle Stiff that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Ceruloplasmin Antibody (6C3K9)
NBP3-15868
Western Blot: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Analysis of extracts of various cell lines, using Ceruloplasmin antibody (NBP3-15868) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [Ceruloplasmin] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Ceruloplasmin Rabbit mAb at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

MTA2 Antibody
NB100-56483
Western Blot: MTA2 Antibody [NB100-56483] - Lysate from Jurkat cells, in the 1) absence and 2) presence of immunizing peptide, probed with this antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunocytochemistry/Immunofluorescence: MTA2 Antibody [NB100-56483] - This antibody was tested in HEK293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Human, Primate
Applications WB, ICC/IF, IHC

3 Publications
COP Antibody
NB100-856
Immunocytochemistry/Immunofluorescence: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Flow Cytometry: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat Polyclonal
Species Human
Applications Flow, ICC/IF, PEP-ELISA

1 Publication
SI Sucrase-Isomaltase Antibody - BSA Free
NBP1-87581
Immunohistochemistry-Paraffin: SI Sucrase-Isomaltase Antibody [NBP1-87581] - Analysis in human small intestine and stomach tissues. Corresponding SI RNA-seq data are presented for the same tissues.Western Blot: SI Sucrase-Isomaltase Antibody [NBP1-87581] - Analysis in human small intestine tissue.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
Emerin Antibody - BSA Free
NBP1-87692
Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP1-87692] - Staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

5 Publications
HOP Antibody (DS14F5) - BSA Free
NBP1-97503

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

8 Publications
Thromboxane synthase Antibody (OTI2C1)
NBP2-02710
Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.Immunocytochemistry/Immunofluorescence: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Thromboxane synthase.

Mouse Monoclonal
Species Human, Rat
Applications WB, Flow, ICC/IF

1 Publication
SHANK2 Antibody (N23b/49) - BSA Free
NBP2-12913
Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 (pan) Antibody (D) Composite.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

Complement Factor D/Adipsin Antibody [Unconjugated]
AF5430
Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=Bone marrow (BM) Adipsin induces bone marrow adiposity expansion during aging.(A) Immunoblot of Adipsin and Adiponectin from plasma of chow-fed male mice at 6, 24, and 76 weeks of age (L.C. = Coomassie staining of the membrane). (B, C) qPCR analyses of gene expression in the BM from tibia (B) and Cfd expression in the epididymal white adipose tissue and subcutaneous white adipose tissue (C) from chow-fed male mice at 26 and 78 weeks of age (n = 5, 5). *p<0.05 for young vs. aging mice. (D–I) Chow-fed 1-year-old male mice. WT (n = 10) and Adipsin KO (n = 9). (D) Representative osmium tetroxide staining and (E) quantification of femoral MAT; (F, G) femoral bone mineral density (BMD) in the cortical (F) and trabecular (G) regions, and (H, I) bone volume normalized by total voume in the cortical (H) and trabecular (I) regions of the femur determined by μCT scans. **p<0.01 for WT vs. Adipsin KO mice. Data represent mean ± SEM. Two-tailed Student’s t-tests were used for statistical analyses.Metabolic phenotyping of Adipsin KO mice during aging.Chow-fed 1-year-old male mice. WT (n = 10) and Adipsin KO (n = 9). (A) Body weight and composition assessed by EchoMRI; (B) insulin tolerance test; (C) GTT; (D) hematoxylin and eosin staining of femoral marrow adipose tissue. (E, F) Average trabecular number (E) and cortical thickness (F) of femurs determined by μCT scans. Data represent mean ± SEM. Two-tailed Student’s t-test was used for statistical analyses. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34155972), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sheep Polyclonal
Species Mouse
Applications WB, IP

13 Publications
Mesothelin Antibody (420411) [Unconjugated]
MAB32652
HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # <a class=Mesothelin knockout HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # <a class=

Rat Monoclonal
Species Human
Applications ELISA, Flow, CyTOF-ready

3 Publications
Fes Antibody (678527)
MAB6460
Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and HEL 92.1.7 human erythroleukemic cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Fes Monoclonal Antibody (Catalog # MAB6460) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Fes was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Fes Monoclonal Antibody (Catalog # MAB6460) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, IHC

DMPK Antibody (713931)
MAB6967
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DMPK Monoclonal Antibody (Catalog # MAB6967) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB

TNF-alpha [Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

824 Publications
PMEL17/SILV Antibody (HMB45)
NBP2-44520
Western Blot: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV antibody (HMB45).Immunohistochemistry: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - PMEL17 (red) was detected in human skin (melanoma) using PMEL17-PE antibody (1:200) in PBS for 1 hour. Nuclei were stained with DAPI (blue). Image from a verified customer review. Image using the PE format of this antibody.

Mouse Monoclonal
Species Human, Canine (Negative), Rat (Negative)
Applications WB, Flow, ICC/IF

8 Publications
Hsp70 interacting protein HIP Antibody - BSA Free
NBP2-47427
Western Blot: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Analysis using Anti-ST13 antibody NBP2-47427 (A) shows similar pattern to independent antibody NBP2-48865 (B).Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody [NBP2-47427] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.

Rabbit Polyclonal
Species Human, Canine
Applications WB, ICC/IF, IHC

1 Publication
ROM1 Antibody - BSA Free
NBP2-58068
Immunocytochemistry/Immunofluorescence: ROM1 Antibody [NBP2-58068] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol.Immunohistochemistry-Paraffin: ROM1 Antibody [NBP2-58068] - Staining of human retina shows strong cytoplasmic positivity in photoreceptor layer.

Rabbit Polyclonal
Species Human, Rat
Applications ICC/IF, IHC, IHC-P

STI1 Antibody (4B6) - Azide and BSA Free
H00010963-M01
Western Blot: STI1 Antibody (4B6) [H00010963-M01] - STIP1 monoclonal antibody (M01), clone 4B6 Analysis of STIP1 expression in HeLa.Immunocytochemistry/Immunofluorescence: STI1 Antibody (4B6) [H00010963-M01] - Analysis of monoclonal antibody to STIP1 on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

5 Publications