CRISPR-Cas9 Antibody Packs
We offer CRISPR-Cas9 Antibody Packs for use in common research applications: Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot. Each CRISPR-Cas9 Antibody Pack is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CRISPR-Cas9 Antibody Packs can be used in a variety of model species: Bacteria. Use the list below to choose the CRISPR-Cas9 Antibody Pack which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CRISPR-Cas9 Antibody Packs.
Alternate Names for CRISPR-Cas9 Antibody Packs
CRISPR-Cas9 antibody pack, Cas9 antibody pack, CRISPR antibody pack, CRISPR-associated protein 9 nuclease antibody pack
1 Results for "crispr-cas9" in Antibody Packs
![Immunocytochemistry/Immunofluorescence: CRISPR-Cas9 Antibody Pack [NBP2-52986] - Hela cells or Hela cells expressing Flag-tagged SpCas9 under the control of the PTight (Tet-ON) promoter were treated for 24h with 1ug/ul Doxycyclin, fixed and permeabilized with Methanol/Acetone and blocked in 2% BSA in PBS for 2 hours at RT. Cells were stained with 6G12 hybridoma supernatant (diluted 1:10) at 4C o/n, followed by incubation with anti mouse-AF488 coupled secondary antibody for 1 h at RT. Nuclei were counter-stained with Hoechst 33342.](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP2-52986-img0004.jpg "Immunocytochemistry/Immunofluorescence: CRISPR-Cas9 Antibody Pack [NBP2-52986] - Hela cells or Hela cells expressing Flag-tagged SpCas9 under the control of the PTight (Tet-ON) promoter were treated for 24h with 1ug/ul Doxycyclin, fixed and permeabilized with Methanol/Acetone and blocked in 2% BSA in PBS for 2 hours at RT. Cells were stained with 6G12 hybridoma supernatant (diluted 1:10) at 4C o/n, followed by incubation with anti mouse-AF488 coupled secondary antibody for 1 h at RT. Nuclei were counter-stained with Hoechst 33342.")
![Western Blot: CRISPR-Cas9 Antibody Pack [NBP2-52986] - Hela cells and Hela cells expressing Flag-tagged S.pyogenes Cas9 under the control of the PTight (Tet-ON) promoter were treated for 24h with 1ug/ul Doxycyclin and lysed under native conditions. 30ug of whole cell lysate per lane was separated by 7.5% SDS-PAGE, transfered to nitrocellulose membrane and incubated with 6G12 hybridoma supernatant (diluted 1:100) at 4C o/n. After washing, the membranes were incubated with secondary HRP-coupled antibody and bands were visualized by ECL and exposure of X-ray films. Prestained marker bands were visualized with Blue Marker Antibody (NBP2-33376). A 1 min exposure is shown. Note the specific band of CRISPR-Cas9 with a molecular weight of 158.4 kDa.](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Pack-Western-Blot-NBP2-52986-img0005.jpg)
![Chromatin Immunoprecipitation (ChIP): CRISPR-Cas9 Antibody Pack [NBP2-52986] - ChIP analysis of CRISPR-Cas9 antibody clones 7A9-3A3 and 6G12 on NIH3T3 cells which were stably expressing GFP-H2B, nuclease dead Cas9 (Flag tagged), and a GFP-targeting gRNA. The cells were fixed with formaldehyde, harvested and sonicated to get 200-500bp DNA fragments. 50ug chromatin was incubated over night at 4C with the indicated antibodies (200ul hybridoma supernatants of clones 7A9 and 6G12; 5ug Flag antibody) followed by incubation with protein G beads for 3h at 4C. After washing chromatin was eluted from the beads and crosslinking was reversed over night at 65C. After a proteinase K digestion step, DNA was separated using phenol/chloroform/isoamyl alcohol, precipitated with ethanol/sodium acetate and dissolved in water. For qPCR, primers either targeting the GFP gene or as negative control non-targeted regions (Ppap2c +7122 and Prkcd +24069 from transcription start) were used.](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Pack-Chromatin-Immunoprecipitation-ChIP-NBP2-52986-img0001.jpg)
![Western Blot: CRISPR-Cas9 Antibody Pack [NBP2-52986] - WB analysis of Cas9 protein in lysate of CRISPR-Cas9 transfected 293T cells using anti-Cas9 antibody clone 7A9-3A3 at 2ug/ml concentration.](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Pack-Western-Blot-NBP2-52986-img0003.jpg)
![Immunoprecipitation: CRISPR-Cas9 Antibody Pack [NBP2-52986] - HEK293T expressing N-terminally Flag-tagged S.pyogenes Cas9 were lysed 72h post transfection by resuspending the cells in Hunt buffer and subjecting to 3 freeze-thaw cycles in liquid nitrogen/ice. Proteins were immunoprecipitated from 100ug of whole cell lysate for 1h at 4C with Cas9 supernatant followed by incubation for 1h at 4C with a 1:1 mixture of protein A/G sepharose beads, or for 2h at 4C with Cas9 ab crosslinked to a 1:1 mixture of protein A/G sepharose beads. Beads were washed 2x with Hunt buffer and 1x with TBS. Bound proteins were eluted by boiling in Laemmli, separated by SDS-PAGE and transferred to nitrocellulose. Membrane was blocked, incubated with Cas9 ab, incubated with HRP anti-mouse secondary. *IgG heavy chain.](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Pack-Immunoprecipitation-NBP2-52986-img0002.jpg)