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Recently, we at Novus Biologicals have added a new batch of monoclonal anti-HIF-1 alpha antibody to our hypoxia catalogue, developed from H1alpha67 hybridoma cultures. This furthers the role that HIF-1 antibodies play in cancer research.

Each week, our lab staff at Novus Biologicals produce, conjugate and purify hundreds of products for our antibody catalogue. Purification protocols are specific to each antibody. For example, we recently purified rabbit polyclonal anti-OCT4 to a final concentration of 1.2 mg/m, using a peptide affinity column.

Hypoxia inhibiting factors protect the cell from death. Autophagy proteins do the opposite. However, hypoxia-induced autophagy has been shown to have a role in cell survival by targeting only the organelles which are oxygen-demanding i.e. mitochondria. Although useful, the mechanism has been implicated in the development of tumours, therefore antibodies against the relevant proteins feature highly in the cancer research pages of our antibody catalogue.

Not everyone knows how antibodies influence animal life, so this article is, in part, a very brief introduction. Apoptosis is triggered by a variety of signals and takes place by one of several mechanisms. These involve cell surface receptors, cytokines, tumour necrosis factors, proteins such as Bcl-2, Bax and Apaf-1, the caspase cascade, and apoptosis-inducing factors.

Researchers requiring antibody products can have some difficulty in obtaining precisely what they need. However, recent developments may make this dilemma less of an issue than it has proven in the past.