The Interleukin 2 Receptor alpha and beta chains, along with the common gamma chain, comprise the high affinity IL2 receptor(IL2R) that is present on both mature activated T- and B-cells, as well as early progenitors of T- and B-cells (thymocytes, pre B-cells, and T-regulatory cells). Formation of homodimeric alpha chains (IL2RA) results in a low affinity receptor, while homodimeric beta chains (IL2RB) produce a medium affinity receptor. IL2RA is normally an integral membrane protein, but a soluble IL2 Receptor alpha has been isolated and appears as the result of extracellular proteolysis. IL2RA plays a key role in lymphocyte differentiation, activation, and proliferation.
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Immunohistochemistry-Paraffin: IL2RA/CD25 Antibody
A standard processing and staining procedure to combat the known heterogeneity of human pancreatic islets was established using the IL2RA/CD25 antibody1. This rigorous method allowed for an extensive cross sectional analysis of pancreatic regions with respect to exocrine, ductular, and endocrine components. Resulting data was then curated into an online database collection for type 1 diabetes researchers. The IL2RA/CD25 antibody was also used in magnetic resonance (MR) scanning of dendritic cell (DC) migration in mice lymph nodes2. Joo’s group used a clinical MR scanner to noninvasively and dynamically follow prostaglandin E2 (PGE2)-induced DC migration to help reduce the costs of predictive DC therapy. Additionally, Crohn’s researchers employed the IL2RA/CD25 antibody to methodically evaluate and profile early pre-clinical disease in hopes of using early intervention to minimize disease severity3. Immunohistochemistry of various cell types and cytokines was performed in hopes of shedding etiological and pathogenesis data on early disease state and correlations between inflammation and other hallmarks.
Novus Biologicals offers IL2RA/CD25 reagents for your research needs including:
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