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PDX-1/IPF1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: PDX-1/IPF1 Antibody [NBP2-38865] - Staining of human cell line HeLa shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody [NBP2-38865] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry: PDX-1/IPF1 Antibody [NBP2-38865] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and islets of Langerhans.
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody [NBP2-38865] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody [NBP2-38865] - Staining of human prostate shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody [NBP2-38865] - Analysis in human duodenum and cerebral cortex tissues using NBP2-38865 antibody. Corresponding PDX1 RNA-seq data are ...read more
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody [NBP2-38865] - Staining of human pancreas shows strong nuclear positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody [NBP2-38865] - Staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody [NBP2-38865] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

PDX-1/IPF1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DISPYEVPPLADDPAVAHLHHHLPAQLALPHPPAGPFPEGAEPGVLEEPNRVQLPFPWMKSTKA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PDX-1/IPF1 Protein (NBP2-38865PEP)
Publications
Read Publications using
NBP2-38865 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (84%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDX-1/IPF1 Antibody

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

PDX1 (Pancrease/duodenum homeobox 1, Insulin promoter factor 1, IPF1, Islet/duodenum homeobox 1, IDX1, Somatatostatin transactivating factor 1, STF1, Insulin upstream factor 1, UUF1, Glucose-sensitive factor, GSF) activates transcription of the insulin and somatostain genes. PDX1 is required for maintaining the hormone-producing phenotype of the beta-cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDX-1/IPF1 Antibody (NBP2-38865)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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(1)
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for PDX-1/IPF1 Antibody (NBP2-38865) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for PDX-1/IPF1 Antibody (NBP2-38865)

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Blogs on PDX-1/IPF1

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Bioinformatics

Gene Symbol PDX1
Uniprot