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PDX-1/IPF1 Antibody (267712) [Unconjugated]

 (12 Publications)
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Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX-1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by ...read more
PDX-1/IPF1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL ...read more
beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419, filled histogram) or isotype control antibody (MAB0041, open histogram) followed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, Flow, ICC/IF
Clone
267712
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2419U-100UG
Alexa Fluor 405
IC2419V-100UG
Alexa Fluor 488
IC2419G-100UG
Alexa Fluor 532
FAB2419X
Alexa Fluor 594
IC2419T-100UG
Alexa Fluor 647
IC2419R-100UG
Alexa Fluor 700
IC2419N-100UG
Alexa Fluor 750
IC2419S-100UG
Allophycocyanin
IC2419A
Biotin
FAB2419B
CoraFluor 1
FAB2419CL1
DyLight 350
FAB2419D
DyLight 405
FAB2419E
DyLight 488
FAB2419K
DyLight 550
FAB2419L
DyLight 594
FAB2419M
DyLight 650
FAB2419W
DyLight 680
FAB2419Y
DyLight 755
FAB2419Z
FITC
FAB2419F
HRP
FAB2419H
Janelia Fluor 525
FAB2419JF525
Janelia Fluor 549
FAB2419I
Janelia Fluor 585
FAB2419JF585
Janelia Fluor 635
FAB2419JF635
Janelia Fluor 646
FAB2419J
Janelia Fluor 669
FAB2419JF669
PerCP
FAB2419C
Phycoerythrin
IC2419P
mFluor Violet 450 SE
FAB2419MFV450
mFluor Violet 500 SE
FAB2419MFV500
mFluor Violet 610 SE
FAB2419MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC2419A
Carrier Free
IC2419P
Carrier Free
MAB2419-SP
Carrier Free
IC2419V-100UG
Carrier Free
IC2419U-100UG
Carrier Free
IC2419T-100UG
Carrier Free
IC2419S-100UG
Carrier Free
IC2419R-100UG
Carrier Free
IC2419N-100UG
Carrier Free
IC2419G-100UG
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PDX-1/IPF1 Antibody (267712) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PDX-1
Ala91-Arg283
Accession # P52945
Specificity
Detects human and mouse PDX-1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PDX1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody (267712) [Unconjugated]

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PDX-1/IPF1 Antibody (267712) [Unconjugated]
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Publications for PDX-1/IPF1 Antibody (MAB2419)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Western Blot.


Filter By Application
ICC
(3)
IHC
(1)
IHC-P
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB2419 Applications Species
Tincy Simon, Pamela Riemer, Armin Jarosch, Katharina Detjen, Annunziata Di Domenico, Felix Bormann et al. DNA methylation reveals distinct cells of origin for pancreatic neuroendocrine carcinomas and pancreatic neuroendocrine tumors Genome Medicine 3/1/2022 [PMID: 35227293]
ET Usher, N Sabri, R Rohac, AK Boal, T Mittag, SA Showalter Intrinsically disordered substrates dictate SPOP subnuclear localization and ubiquitination activity The Journal of Biological Chemistry, 2021-04-22;0(0):100693. 2021-04-22 [PMID: 33894201] (Western Blot, Human) Western Blot Human
Annunziata Di Domenico, Christodoulos P. Pipinikas, Renaud S. Maire, Konstantin Bräutigam, Cedric Simillion, Matthias S. Dettmer et al. Epigenetic landscape of pancreatic neuroendocrine tumours reveals distinct cells of origin and means of tumour progression Communications Biology 12/7/2020 [PMID: 33288854]
Bernard H, Teijeiro A, Chaves-Perez A et al. Coxsackievirus B Type 4 Infection in beta Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing Cell Reports Medicine 10/1/2020 [PMID: 33205075]
Ralph Gruber, Richard Panayiotou, Emma Nye, Bradley Spencer-Dene, Gordon Stamp, Axel Behrens YAP1 and TAZ Control Pancreatic Cancer Initiation in Mice by Direct Up-regulation of JAK–STAT3 Signaling Gastroenterology 9/1/2016 [PMID: 27215660]
Shruti Balaji, Yu Zhou, Anasuya Ganguly, Emmanuel C. Opara, Shay Soker The combined effect of PDX1, epidermal growth factor and poly-L-ornithine on human amnion epithelial cells’ differentiation BMC Developmental Biology 12/1/2016 [PMID: 27068127]
P Saxena, BC Heng, P Bai, M Folcher, H Zulewski, M Fussenegge A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells Nat Commun, 2016-04-11;7(0):11247. 2016-04-11 [PMID: 27063289] (IHC, Human) IHC Human
Lenz A, Toren-Haritan G, Efrat S Redifferentiation of adult human beta cells expanded in vitro by inhibition of the WNT pathway. PLoS ONE, 2014-11-13;9(11):e112914. 2014-11-13 [PMID: 25393025] (ICC, Human) ICC Human
Jason Y. Park, Seung-Mo Hong, David S. Klimstra, Michael G. Goggins, Anirban Maitra, Ralph H. Hruban Pdx1 Expression in Pancreatic Precursor Lesions and Neoplasms Applied Immunohistochemistry & Molecular Morphology 10/1/2011 [PMID: 21297446]
Evans-Molina C, Robbins RD, Kono T, Tersey SA, Vestermark GL, Nunemaker CS, Garmey JC, Deering TG, Keller SR, Maier B, Mirmira RG Peroxisome proliferator-activated receptor gamma activation restores islet function in diabetic mice through reduction of endoplasmic reticulum stress and maintenance of euchromatin structure. Mol. Cell. Biol., 2009-02-23;29(8):2053-67. 2009-02-23 [PMID: 19237535] (IHC-P, Mouse) IHC-P Mouse
Show All 12 Publications.

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Bioinformatics

Gene Symbol PDX1
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