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Olig2 Antibody [mFluor Violet 500 SE]

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Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
mFluor Violet 500 SE

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Olig2 Antibody [mFluor Violet 500 SE] Summary

Immunogen
Antibody was raised against a 15 amino acid peptide near the amino terminus of human OLIG2. The immunogen is located within amino acids 80 - 130 of OLIG2. Amino Acid Squence: STKKDKKQMTEPELQ
Specificity
It is predicted to not cross-react with other members of the OLIG family of proteins. At least three isoforms of OLIG2 are known to exist; this antibody will detect all three isoforms.
Predicted Species
Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OLIG2
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Olig2 Antibody [mFluor Violet 500 SE]

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol OLIG2