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Olig2 Antibody (OLIG2/6695R)

Images

 
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - Formalin-fixed, paraffin-embedded human glioma stained withOlig2 antibody (OLIG2/6695R).
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - Formalin-fixed, paraffin-embedded human cerebrum stained with Olig2 antibody (OLIG2/6695R).
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - Formalin-fixed, paraffin-embedded human oligodendroglioma stained with Olig2 antibody (OLIG2/6695R).
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - Formalin-fixed, paraffin-embedded human cerebellum stained with Olig2 antibody (OLIG2/6695R).
Immunohistochemistry-Paraffin: Olig2 Antibody (OLIG2/6695R) [NBP3-13830] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Stained with Olig2 antibody (OLIG2/6695R) at 2ug/ml in PBS for 30min RT. Inset: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
OLIG2/6695R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Olig2 Antibody (OLIG2/6695R) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14217)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (around aa200-300) of human Olig2 protein (exact sequence is proprietary) (Uniprot: Q13516)
Localization
Nucleus, Cytoplasm
Marker
Marker of Glial Brain Tumors
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
OLIG2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Olig2 Antibody (OLIG2/6695R)

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol OLIG2
Uniprot