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Olig2 Antibody (064)

Images

 
Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey ...read more
Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human brain with rabbit monoclonal antibody (1:200).
Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human glioma with rabbit monoclonal antibody (1:200).
Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Mouse brain cells fixed and stained for Olig2 protein: 1:100. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a ...read more
Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Rat brain cells fixed and stained with Olig2. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
064
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Olig2 Antibody (064) Summary

Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, synthetic peptide corresponding to the N-terminus of the Human Olig2.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
OLIG2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NBP2-89201 in the following applications:

Publications
Read Publications using
NBP2-89201 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for Olig2 Antibody (064)

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Olig2 Antibody (NBP2-89201)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IHC-P.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
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Mouse
(1)
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Showing Publications 1 - 5 of 5.
Publications using NBP2-89201 Applications Species
Qiu X, Ping S, Kyle M et al. Stem cell factor and granulocyte colony-stimulating factor promote remyelination in the chronic phase of severe traumatic brain injury bioRxiv 2023-01-25 [PMID: 36899841]
Scarian E 3D Bioprinting and organoids: a new era in the study of Amyotrophic Lateral Sclerosis Thesis 2023-01-01
Maniou E, Farah F, Crane-Smith Z et al. CaudalFgfr1disruption produces localised spinal mis-patterning and a terminal myelocystocele-like phenotype in mice bioRxiv 2023-03-08 (IHC-P) IHC-P
Nilsson G, Mottahedin A, Zelco A et al. Two different isoforms of osteopontin modulate myelination and axonal integrity FASEB BioAdvances 2023-06-17 [PMID: 37554545] (ICC/IF, Mouse) ICC/IF Mouse
Lee JS The role of QKI and PRMT1 in microglia during de/remyelination Thesis Jan 1 2022

Review for Olig2 Antibody (NBP2-89201) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-89201:
Filter by Applications
Flow
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
Flow Mouse 04/02/2024
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Summary

ApplicationFlow Cytometry
Sample TestedAdult mouse brain
SpeciesMouse
LotD152404

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***

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Verified Customer
04/02/2024
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol OLIG2