![Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - Nse2 Antibody [NBP1-76263] - CRISPR-Cas9-mediated targeting of NSMCE2. Western blot analysis of the NSMCE2 protein in the final NSMCE2 null and WT cells. beta-Actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/17/11/1782) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nse2-Antibody-Western-Blot-NBP1-76263-img0002.jpg "Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - Nse2 Antibody [NBP1-76263] - CRISPR-Cas9-mediated targeting of NSMCE2. Western blot analysis of the NSMCE2 protein in the final NSMCE2 null and WT cells. beta-Actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/17/11/1782) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MMS21. The immunogen is located within amino acids 170 - 220 of MMS21. Amino Acid Squence: CIVRMIESRQKRKKKA |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NSMCE2 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
| Publications using NBP1-76263 | Applications | Species |
|---|---|---|
| Verver DE, Zheng Y, Speijer D et al. Non-SMC Element 2 (NSMCE2) of the SMC5/6 Complex Helps to Resolve Topological Stress. Int J Mol Sci 2016-10-26 [PMID: 27792189] (WB, Human) | WB | Human |
| Cho T, Zhao Y, Olivieri M et al. Chemogenetic profiling of ubiquitin-like modifier pathways identifies NFATC2IP as a mediator of SUMO-dependent genome integrity bioRxiv 2023-07-01 (WB, Human) Details: 1:1000 dilution |
WB | Human |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - Nse2 Antibody [NBP1-76263] - 293 cell lysate with Nse2 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/Nse2-Antibody-Western-Blot-NBP1-76263-img0001.jpg "Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - Nse2 Antibody [NBP1-76263] - 293 cell lysate with Nse2 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - CRISPR-Cas9-mediated targeting of NSMCE2: (A) Left panel, transfected U2OS cells (GFP+) under bright field & fluorescence; right panel, FACS enrichment of GFP+ cells. Bar = 50 µm; (B) Expected cleavage bands (approximately 304 & 113 bp, arrowheads) generated by Surveyor nuclease digestion. For negative control (−) transfection of the pX458 plasmids without NSMCE2 sgRNA was performed; (C) Sequencing analysis for characterization of the CRISPR-Cas9-induced frameshift mutations. Red letters represent the 20-nt targeting sequences, while blue letters refer to the protospacer-adjacent motif (PAM); (D) Western blot analysis of the NSMCE2 protein in the final NSMCE2 null & WT cells. beta -Actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/17/11/1782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76263_rabbit-polyclonal-nse2-antibody-31020241529335.jpg "Western Blot: Nse2 Antibody - BSA Free [NBP1-76263] - CRISPR-Cas9-mediated targeting of NSMCE2: (A) Left panel, transfected U2OS cells (GFP+) under bright field & fluorescence; right panel, FACS enrichment of GFP+ cells. Bar = 50 µm; (B) Expected cleavage bands (approximately 304 & 113 bp, arrowheads) generated by Surveyor nuclease digestion. For negative control (−) transfection of the pX458 plasmids without NSMCE2 sgRNA was performed; (C) Sequencing analysis for characterization of the CRISPR-Cas9-induced frameshift mutations. Red letters represent the 20-nt targeting sequences, while blue letters refer to the protospacer-adjacent motif (PAM); (D) Western blot analysis of the NSMCE2 protein in the final NSMCE2 null & WT cells. beta -Actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/17/11/1782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")