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SMC6L1 Antibody

Images

 
Western Blot: SMC6L1 Antibody [NBP2-20420] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC6L1 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: SMC6L1 Antibody [NBP2-20420] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SMC6L1 Antibody [NBP2-20420] - Mouse testis [N3C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: SMC6L1 Antibody [NBP2-20420] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SMC6L1 Antibody [NBP2-20420] - Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SMC6L1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SMC6L1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC6
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for SMC6L1 Antibody

  • FLJ22116
  • hSMC6
  • SMC protein 6
  • SMC6 structural maintenance of chromosomes 6-like 1 (yeast)
  • SMC6 structural maintenance of chromosomes 6-like 1
  • SMC-6
  • SMC6L1FLJ35534
  • structural maintenance of chromosomes 6
  • structural maintenance of chromosomes protein 6

Background

Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMC6
Uniprot