![Western Blot: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nse2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nse2.](http://images.novusbio.com/fullsize/Nse2-Antibody-OTI6A6-Azide-and-BSA-Free-Western-Blot-NBP2-73070-img0001.jpg "Western Blot: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nse2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nse2.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, CyTOF-ready |
| Clone | OTI6A6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human NSMCE2 (NP_775956) produced in E.coli. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NSMCE2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 27.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NSMCE2 |
![Immunocytochemistry/Immunofluorescence: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nse2.](http://images.novusbio.com/fullsize/Nse2-Antibody-OTI6A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73070-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nse2.")
![Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Nse2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Nse2-Antibody-OTI6A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73070-img0003.jpg "Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Nse2 antibody, and then analyzed by flow cytometry.")
![Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - Analysis of Jurkat cells, using anti-Nse2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Nse2-Antibody-OTI6A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73070-img0002.jpg "Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - Analysis of Jurkat cells, using anti-Nse2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
