Shake bottle vigorously or vortex briefly before use.
1. Add the monoclonal antibody of your choice to 100 uL of test sample.
2. Incubate, lyse, wash, and then resuspend in 1 to 2 mL PBS. If staining and lysing are not needed, just mix a known volume of test sample in 1 to 2 mL PBS
3. Add exactly 50 uL of NBP3-00495 particles to the suspension. Precision when pipetting is absolutely critical.
4. Run the sample in the flow cytometer and obtain the amount of events for the NBP3-00495 particles and your test sample.
5. Calculate the number of cells accordingly:
(A/B) x (C/D) = Number of cells per uL
where
A = number of events for the test sample
B = number of events for the NBP3-00495 particles
C = number of NBP3-00495 particles per 50 uL
D = volume of test sample initally used
