Hu,
Mu,
Rt,
Ba,
Ba,
Ch,
Ec,
Gp,
Ha,
In,
Pa,
Pl,
Pm,
YeSpecies Glossary
Reactivity: Hu, Mu, Rt, Ba, Ba, Ch, Ec, Gp, Ha, In, Pa, Pl, Pm, Ye
WB, Simple Western, ICC/IF, IHC, WB (-)
Applications: WB, Simple Western, ICC/IF, IHC, WB (-)
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0002.jpg "Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Western blot analysis of Hep2 cell lysate using HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Western-Blot-NBP2-32867-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867] - Immunofluorescent staining of MEOH-fixed HeLa cells using followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunocytochemistry-Immunofluorescence-NBP2-32867-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) [NBP2-32867] - Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Immunohistochemistry-Paraffin-NBP2-32867-img0008.jpg)
![Western Blot (Negative): HSP60 Antibody (LK2) [NBP2-32867] - Immunoblot analysis of whole bacterial lysate of Mtb cells pretreated with (+) or without (-) proteinase K followed by incubation with K632-7 poly-Ub chains at 4C for 4 hr. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-09955-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Western-Blot-Negative-NBP2-32867-img0006.jpg)
![Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Simple-Western-NBP2-32867-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) - Azide and BSA Free [NBP2-34671] - Immunofluorescent staining of MEOH-fixed HeLa cells using HSP60 Antibody (LK2) followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34671-img0003.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) - Azide and BSA Free [NBP2-34671] - Immunofluorescent staining of MEOH-fixed HeLa cells using HSP60 Antibody (LK2) followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).")
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK2) - Azide and BSA Free [NBP2-34671] - Formalin-fixed, paraffin-embedded human liver in colon stained with HSP60 Mouse Monoclonal Antibody (LK2).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34671-img0004.jpg)
![Simple Western: HSP60 Antibody (LK2) - Azide and BSA Free [NBP2-34671] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Azide-and-BSA-Free-Simple-Western-NBP2-34671-img0001.jpg)
![Simple Western: HSP60 Antibody (LK2) - Azide and BSA Free [NBP2-34671] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK2-Azide-and-BSA-Free-Simple-Western-NBP2-34671-img0002.jpg)
![Western Blot: CRISPR-Cas9 Antibody (JM11-55) [NBP2-67000] - Analysis of CRISPR-Cas9 SP on 293 and 293T cells lysates transfected with CRISPR-Cas9 using anti- CRISPR-Cas9 SP antibody at 1:500 dilution. Expected molecular weight: 158.4 kDa.](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-JM11-55-Western-Blot-NBP2-67000-img0001.jpg "Western Blot: CRISPR-Cas9 Antibody (JM11-55) [NBP2-67000] - Analysis of CRISPR-Cas9 SP on 293 and 293T cells lysates transfected with CRISPR-Cas9 using anti- CRISPR-Cas9 SP antibody at 1:500 dilution. Expected molecular weight: 158.4 kDa.")
![Western Blot: CRISPR-Cas9 Antibody [NBP2-52718] - Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15ug per lane. Primary Antibody cas9 used at 1ug/mL overnight at 4C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expected theoretical molecular weight: 158 kDa.](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Western-Blot-NBP2-52718-img0001.jpg "Western Blot: CRISPR-Cas9 Antibody [NBP2-52718] - Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15ug per lane. Primary Antibody cas9 used at 1ug/mL overnight at 4C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expected theoretical molecular weight: 158 kDa.")
![Immunofluorescence: CRISPR-Cas9 Antibody [NBP2-52718] - HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1ug/mL. Secondary: Donkey anti-Rabbit at 1ug/mL, staining seen in red. Bottom images show GFP stain overlay](http://images.novusbio.com/fullsize/CRISPR-Cas9-Antibody-Immunofluorescence-NBP2-52718-img0002.jpg)