Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ch,
Pm,
SyHa,
Gp,
Pm-Or,
Rb,
Sh,
XpSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, SyHa, Gp, Pm-Or, Rb, Sh, Xp
WB, Simple Western, IHC, ICC/IF (-), IP (-)
Applications: WB, Simple Western, IHC, ICC/IF (-), IP (-)
Host: Rabbit Polyclonal
![Simple Western: beta-Actin Antibody [NB600-532] - Simple Western lane view shows a specific band for Beta Actin using NB600-532 at 1:200 in A431, C2C12 and C6 cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Simple-Western-NB600-532-img0016.jpg "Simple Western: beta-Actin Antibody [NB600-532] - Simple Western lane view shows a specific band for Beta Actin using NB600-532 at 1:200 in A431, C2C12 and C6 cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: beta-Actin Antibody [NB600-532] - Detection of actin in 3T3 lysates (20ug). ECL detection 30 seconds. A specific band is seen using different dilutions: Lane 1 (1:15,000), Lane 2 (1:10,000), and Lane 3 (1:15,000).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0009.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody NB600-532 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0014.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Mouse colon whole cell lysate. PVDF membrane was probed with Rabbit Anti-B actin Antibody (Catalog # NB600-532) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0019.jpg)
![Immunohistochemistry: beta-Actin Antibody [NB600-532] - Detection of human Cytoskeletal Actin by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin (NB600-532). Detection: DAB](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Immunohistochemistry-NB600-532-img0015.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using RCC4 whole cell lysate [NBP1-30412].](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0006.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0007.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Analysis using the HRP conjugate of NB600-532. Detection of Beta Actin in MCF-7 cell lysate (20ug) using anti-Beta Actin antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0011.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Treatments of periplocin and/or TRAIL activate DR4, FADD, and proapoptotic proteins in HCC cells. The effect of periplocin treatment on the expression of DR4, DR5, and FADD was analyzed by western blot. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ecam/2013/958025/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-Western-Blot-NB600-532-img0018.jpg)
![Western Blot: beta-Actin Antibody [NB600-532] - Hypoxic preconditioning increases PBMNC sensitivity to apelin-13 via upregulation of APJ expression, leading to growth factor secretion.(a) APJ protein expression was significantly increased in hypoxic PBMNCs. *p Mol Neurodegener (2020). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-532_rabbit-polyclonal-beta-actin-antibody-23220248135226.jpg)
![Western Blot: GOT2 Antibody [NBP2-32241] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GOT2 antibody NBP2-32241 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GOT2-Antibody-Western-Blot-NBP2-32241-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: GOT2 Antibody [NBP2-32241] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GOT2 antibody NBP2-32241 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- GOT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-NBP2-32241-img0003.jpg)
![Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- GOT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/GOT2-Antibody-Immunohistochemistry-NBP2-32241-img0002.jpg)
![Western Blot: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFWD3 antibody NB100-68208 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFWD3-Antibody-Western-Blot-NB100-68208-img0004.jpg "Western Blot: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFWD3 antibody NB100-68208 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: RFWD3 was immunoprecipitated by rabbit anti-RFWD3 antibodies NB100-68206 and NB100-68207. For blotting immunoprecipitated RFWD3, NB100-68208 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFWD3-Antibody-Immunoprecipitation-NB100-68208-img0005.jpg)
![Western Blot: RPL6 Antibody [NBP2-04036] - Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibody used at 0.04 ug/ml.](http://images.novusbio.com/fullsize/RPL6-Antibody-Western-Blot-NBP2-04036-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPL6 Antibody [NBP2-04036] - Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibody used at 0.04 ug/ml.")