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![Western Blot: HSP60 Antibody [NBP2-12734] - Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734) at 1:1000.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-12734-img0003.jpg "Western Blot: HSP60 Antibody [NBP2-12734] - Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734) at 1:1000.")
![Immunohistochemistry: HSP60 Antibody [NBP2-12734] - Immunohistochemistry analysis using Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734). Tissue: Liver. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (NBP2-12734).](http://images.novusbio.com/fullsize/HSP60 Antibody-Immunohistochemistry-NBP2-12734-img0004.jpg)
![Western Blot: HSP60 Antibody [NBP2-12734] - Analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody . Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP2-12734-img0002.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NBP2-59180-img0001.jpg "Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NBP2-59180] - ChIP assays were performed using human HeLa cells, the antibody against H3K9ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers speci c for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that acetylation of K9 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59180-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys9] Antibody [NBP2-59180] - HeLa cells were stained with the antibody against H3K9ac and with DAPI. Cells were mixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immuno uorescently labelled with the H3K9ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Immunofluorescence-NBP2-59180-img0003.jpg)
![ELISA: Histone H3 [ac Lys9] Antibody [NBP2-59180] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K9ac in antigen coated wells. The antigen used was a peptide containing the histone modi cation of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:31,700.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-ELISA-NBP2-59180-img0005.jpg)
![Western Blot: PfHsp40 Antibody [NBP3-18240] - Western blot analysis of Parasite lysates showing detection of PfHsp40 protein using Rabbit Anti-PfHsp40 Polyclonal Antibody (NBP3-18240). Primary Antibody: Rabbit Anti-PfHsp40 Polyclonal Antibody (NBP3-18240) at 1:2000.](http://images.novusbio.com/fullsize/PfHsp40-Antibody-Western-Blot-NBP3-18240-img0001.jpg "Western Blot: PfHsp40 Antibody [NBP3-18240] - Western blot analysis of Parasite lysates showing detection of PfHsp40 protein using Rabbit Anti-PfHsp40 Polyclonal Antibody (NBP3-18240). Primary Antibody: Rabbit Anti-PfHsp40 Polyclonal Antibody (NBP3-18240) at 1:2000.")
![Western Blot: PfHsp40 Antibody [NBP3-18240] - Western blot analysis of Parasite lysates showing detection of PfHsp40 protein using Rabbit Anti-PfHsp40 Polyclonal Antibody (NBP3-18240). Primary Antibody: Rabbit Anti-PfHsp40 Polyclonal Antibody (NBP3-18240) at 1:2000.](http://images.novusbio.com/fullsize/PfHsp40 Antibody-Western Blot-NBP3-18240-img0002.jpg)
![Western Blot: PfHsp70 Antibody [NBP3-18239] - Western blot analysis of Parasite Lysates showing detection of PfHsp70 protein using Rabbit Anti-PfHsp70 Polyclonal Antibody (NBP3-18239). Primary Antibody: Rabbit Anti-PfHsp70 Polyclonal Antibody (NBP3-18239) at 1:2000.](http://images.novusbio.com/fullsize/PfHsp70-Antibody-Western-Blot-NBP3-18239-img0001.jpg "Western Blot: PfHsp70 Antibody [NBP3-18239] - Western blot analysis of Parasite Lysates showing detection of PfHsp70 protein using Rabbit Anti-PfHsp70 Polyclonal Antibody (NBP3-18239). Primary Antibody: Rabbit Anti-PfHsp70 Polyclonal Antibody (NBP3-18239) at 1:2000.")
![Western Blot: PfHsp70 Antibody [NBP3-18239] - Western blot analysis of Parasite Lysates showing detection of PfHsp70 protein using Rabbit Anti-PfHsp70 Polyclonal Antibody (NBP3-18239). Primary Antibody: Rabbit Anti-PfHsp70 Polyclonal Antibody (NBP3-18239) at 1:2000.](http://images.novusbio.com/fullsize/PfHsp70 Antibody-Western Blot-NBP3-18239-img0002.jpg)