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9 Results for "novus"
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AF1018

ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated]

(1 Review)
(41 Publications)
100 μg
AF1018
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, Flow, IHC, CyTOF-ready
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1018-SP
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AF1576

ERK1/ERK2 Antibody [Unconjugated]

(1 Review)
(30 Publications)
100 μg
AF1576
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western
Applications: WB, Simple Western
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1576-SP
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MAB1018

ERK1/ERK2 [p Tyr187, p Tyr204, p Thr185, p Thr202] Antibody (269434) [Unconjugated]

(20 Publications)
100 μg
MAB1018
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Rabbit Anti-Human PDGF (Catalog # 120-HD) and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) and HeLa human cervical epithelial carcinoma cell line untreated or treated with 200 nM PMA. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (MAB1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, CyTOF-ready, Flow, ICC/IF
Applications: WB, Simple Western, CyTOF-ready, Flow, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB1018U
Alexa Fluor 350 IC1018U-100UG
Alexa Fluor 405 FAB1018V
Alexa Fluor 405 IC1018V-100UG
Alexa Fluor 488 IC7806G-100UG
Alexa Fluor 532 FAB1018X
Alexa Fluor 594 FAB1018T
Alexa Fluor 594 IC1018T-100UG
Alexa Fluor 647 IC1018R-100UG
Alexa Fluor 647 FAB1018R
Alexa Fluor 700 IC1018N-100UG
Alexa Fluor 700 FAB1018N
Alexa Fluor 750 FAB1018S
Alexa Fluor 750 IC1018S-100UG
Allophycocyanin FAB1018A
Biotin FAB1018B
DyLight 350 FAB1018D
DyLight 405 FAB1018E
DyLight 488 FAB1018K
DyLight 550 FAB1018L
DyLight 594 FAB1018M
DyLight 650 FAB1018W
DyLight 680 FAB1018Y
DyLight 755 FAB1018Z
FITC FAB1018F
HRP FAB1018H
Janelia Fluor 549 FAB1018I
Janelia Fluor 646 FAB1018J
PE FAB1018P
PerCP FAB1018C
mFluor Violet 450 SE FAB1018MFV450
mFluor Violet 500 SE FAB1018MFV500
mFluor Violet 610 SE FAB1018MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC7806G-100UG
Carrier Free MAB1018-SP
Carrier Free IC1018V-100UG
Carrier Free IC1018U-100UG
Carrier Free IC1018T-100UG
Carrier Free IC1018S-100UG
Carrier Free IC1018R-100UG
Carrier Free IC1018N-100UG
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MAB1576

ERK1/ERK2 Antibody (216703) [Unconjugated]

(3 Reviews)
(19 Publications)
100 μg
MAB1576
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC
Applications: WB, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1576-SP
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MAB15761

ERK1/ERK2 Antibody (631122)

(1 Review)
(3 Publications)
100 μg
MAB15761
Western blot shows lysates of MCF-7 human breast cancer cell line, BaF3 mouse pro-B cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB15761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for ERK1/ERK2 at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB15761-SP
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NBP3-21670

ERK1/ERK2 Antibody (SR1700)

100 ul
NBP3-21670
Western Blot: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] - Western blot analysis using NBP3-21670 in HepG2 whole cell lysates.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Flow, ICC/IF, IP
Applications: WB, Flow, ICC/IF, IP
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
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NBP3-21588

ERK1/ERK2 [p Thr202] Antibody (SR1701)

100 ul
NBP3-21588
Western Blot: ERK1/ERK2 [p Thr 202] Antibody (SR1701) [NBP3-21588] - Western blot analysis using NBP3-21588 in (1) NIH/3T3 cell lysate; (2) NIH/3T3 cell lysate treated with PDGF.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IHC, IP
Applications: WB, ICC/IF, IHC, IP
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
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NBP3-13137

ERK1/ERK2 [p Thr185, p Tyr187] Antibody

100 ul
NBP3-13137
Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) / ERK2 (phospho Thr185/Tyr187) antibody (NBP3-13137) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Hu, Mu
Species Glossary
Reactivity: Hu, Mu
WB
Applications: WB
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
         
Validated by:          
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NBP3-21522

ERK1/ERK2 [p Tyr204] Antibody (SR1718)

100 ul
NBP3-21522
Western Blot: ERK1/ERK2 [p Tyr 204] Antibody (SR1718) [NBP3-21522] - Western blot analysis using NBP3-21522 in A431 cell lysate treated with EGF.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IP
Applications: WB, IP
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
compare
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