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![Western Blot: Human Coronavirus Spike Protein Antibody (23) [NBP3-14657] - Anti-Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His Secondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-23-Western-Blot-NBP3-14657-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (23) [NBP3-14657] - Anti-Coronavirus Spike Protein mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His Lane 12: HCoV-OC43 Spike S1+S2 ECD-His Secondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Spike Protein Antibody (06) [NBP3-14654] - Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-Protein-Antibody-06-Western-Blot-NBP3-14654-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (06) [NBP3-14654] - Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-HisLane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVI Lane 3: SARS-CoV-2 Spike S1 NTD-His & AVI Lane 4: SARS-CoV-2 Spike RBD-His Lane 5: SARS-CoV-2 Spike S2 ECD-His Lane 6: SARS-CoV-2 Spike S1+S2 ECD-His Lane 7: SARS-CoV Spike S1+S2 ECD-His Lane 8: MERS-CoV Spike S1+S2 ECD-His Lane 9: HCoV-NL63 Spike S1+S2 ECD-His Lane 10: HCoV-229E Spike S1+S2 ECD-His Lane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-His SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![Western Blot: Human Coronavirus Nucleocapsid Protein Antibody [NBP3-12767] - Sample Human coronavirus (HCoV-NL63) Nucleoprotein / NP ProteinLane A: 5ngLane B: 1ngSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Human-Coronavirus-Nucleocapsid-Protein-Antibody-Western-Blot-NBP3-12767-img0001.jpg "Western Blot: Human Coronavirus Nucleocapsid Protein Antibody [NBP3-12767] - Sample Human coronavirus (HCoV-NL63) Nucleoprotein / NP ProteinLane A: 5ngLane B: 1ngSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
![ELISA: Human Coronavirus Spike S1 Antibody - (HCoV-NL63) [NBP3-14864] - Antigen-down ELISA with HCoV-229E, HCoV-HKU1, HCoV-OC43, HCoV-NL63, SARS-CoV and SARS-CoV-2 spike subunit 1 proteins coated at 0.5ug. Primary antibody dilutions (1000 ng/ml to 0.24 ng/ml) added and 100ul of goat anti rabbit IgG HRP secondary antibody (1:10,000). Detection carried out using TMB substrate (15 min.), reaction stopped and absorbance read at 405/450nm.](http://images.novusbio.com/fullsize/Human-Coronavirus-Spike-S1-Antibody-HCoV-NL63-ELISA-NBP3-14864-img0001.jpg "ELISA: Human Coronavirus Spike S1 Antibody - (HCoV-NL63) [NBP3-14864] - Antigen-down ELISA with HCoV-229E, HCoV-HKU1, HCoV-OC43, HCoV-NL63, SARS-CoV and SARS-CoV-2 spike subunit 1 proteins coated at 0.5ug. Primary antibody dilutions (1000 ng/ml to 0.24 ng/ml) added and 100ul of goat anti rabbit IgG HRP secondary antibody (1:10,000). Detection carried out using TMB substrate (15 min.), reaction stopped and absorbance read at 405/450nm.")