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![Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Western-Blot-NBP2-02472-img0002.jpg "Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.")
![Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP2-02472-img0009.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded prostate tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0008.jpg)
![Flow Cytometry: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells transfected with either pCMV6-ENTRY BTK.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Flow-Cytometry-NBP2-02472-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] - Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP2-02472-img0003.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0004.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0005.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0006.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Kidney tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0007.jpg)
![Western Blot: BTK Antibody (7F12H4) [NB110-87047] - Analysis of BTK expression in 2) Raji, 3) A431 whole cell lysates and 4) human tonsil tissue extract.](http://images.novusbio.com/fullsize/BTK-Antibody-7F12H4-Western-Blot-NB110-87047-img0009.jpg "Western Blot: BTK Antibody (7F12H4) [NB110-87047] - Analysis of BTK expression in 2) Raji, 3) A431 whole cell lysates and 4) human tonsil tissue extract.")
![Immunocytochemistry/Immunofluorescence: BTK Antibody (7F12H4) [NB110-87047] - The BTK antibody was tested in A431 cells against DyLight 488 (Green). Actin and nuclei were counterstained for Phalloidin (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/BTK-Antibody-7F12H4-Immunocytochemistry-Immunofluorescence-NB110-87047-img0010.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (7F12H4) [NB110-87047] - Immunohistochemical analysis of paraffin-embedded human lymph-node tissues (left) and human lymph follicle tissues (right), showing cytoplasmic and membrane localization using BTK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BTK-Antibody-7F12H4-Immunohistochemistry-Paraffin-NB110-87047-img0005.jpg)
![Western Blot: BTK Antibody (7F12H4) [NB110-87047] - Analysis of BTK expression in 1) truncated BTK recombinant protein and 2) K562 cell lysate.](http://images.novusbio.com/fullsize/BTK-Antibody-7F12H4-Western-Blot-NB110-87047-img0006.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89207] - Analysis in human tonsil and kidney tissues. Corresponding BTK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-Paraffin-NBP1-89207-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89207] - Analysis in human tonsil and kidney tissues. Corresponding BTK RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: BTK Antibody [NBP1-89207] - Analysis using Anti-BTK antibody NBP1-89207 (A) shows similar pattern to independent antibody NBP1-89206 (B).](http://images.novusbio.com/fullsize/BTK-Antibody-Western-Blot-NBP1-89207-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: BTK Antibody [NBP1-89207] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89207-img0007.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89207] - Staining of human lymph node shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-NBP1-89207-img0016.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89207] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-Paraffin-NBP1-89207-img0012.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89207] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-Paraffin-NBP1-89207-img0013.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89207] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-NBP1-89207-img0015.jpg)
![Western Blot: BTK Antibody [NBP1-76596] - Analysis of BTK in U937 cell lysate with anti-BTK using NBP1-76596 at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/BTK-Antibody-Western-Blot-NBP1-76596-img0003.jpg "Western Blot: BTK Antibody [NBP1-76596] - Analysis of BTK in U937 cell lysate with anti-BTK using NBP1-76596 at (A) 0.5, (B) 1, and (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: BTK Antibody [NBP1-76596] - BTK in mouse brain tissue with BTK antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76596-img0008.jpg)
![Immunohistochemistry: BTK Antibody [NBP1-76596] - Staining of mouse brain tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-NBP1-76596-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: BTK Antibody [NBP1-76596] - Daudi cells with BTK antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76596-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BTK Antibody [NBP1-76596] - Daudi cells cells with BTK antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76596-img0004.jpg)
![Western Blot: BTK Antibody [NBP1-89206] - Analysis using Anti-BTK antibody NBP1-89206 (A) shows similar pattern to independent antibody NBP1-89207 (B).](http://images.novusbio.com/fullsize/BTK-Antibody-Western-Blot-NBP1-89206-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BTK Antibody [NBP1-89206] - Analysis using Anti-BTK antibody NBP1-89206 (A) shows similar pattern to independent antibody NBP1-89207 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining in human tonsil and skeletal muscle tissues using NBP1-89206 antibody. Corresponding BTK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-Paraffin-NBP1-89206-img0015.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-Paraffin-NBP1-89206-img0011.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human rectum shows moderate membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-Paraffin-NBP1-89206-img0012.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-Paraffin-NBP1-89206-img0013.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody [NBP1-89206] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/BTK-Antibody-Immunohistochemistry-Paraffin-NBP1-89206-img0014.jpg)
![Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Western-Blot-NBP3-15612-img0004.jpg "Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: BTK Antibody (2J9O8) [NBP3-15612] - Immunofluorescence analysis of mouse spleen using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-NBP3-15612-img0003.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded human lung cancer using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0001.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded mouse spleen using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0002.jpg)
![Western Blot: BTK [p Tyr223] Antibody [NBP3-12916] - Untreated (-) and treated (+) Raji whole cell extracts (1 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:6000.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Western-Blot-NBP3-12916-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: BTK [p Tyr223] Antibody [NBP3-12916] - Untreated (-) and treated (+) Raji whole cell extracts (1 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:6000.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Rat lymph node. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0004.jpg)
![Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Mouse spleen. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0002.jpg)
![Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Mouse thymus gland. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BTK-[p-Tyr223]-Antibody-Immunohistochemistry-Paraffin-NBP3-12916-img0003.jpg)
![SDS-Page: Recombinant Human BTK Protein [H00000695-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BTK-Protein-SDS-Page-H00000695-P01-img0001.jpg "SDS-Page: Recombinant Human BTK Protein [H00000695-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: BTK [p Tyr 551] Antibody (SR2188) [NBP3-21550] - Western blot analysis using NBP3-21550 in Ramos cell lysate treated with Pervanadate.](http://images.novusbio.com/fullsize/nbp3-21550_rabbit-btk-p-tyr-551-mab-sr2188-5720231624714.jpg "Western Blot: BTK [p Tyr 551] Antibody (SR2188) [NBP3-21550] - Western blot analysis using NBP3-21550 in Ramos cell lysate treated with Pervanadate.")
![Western Blot: BTK [p Tyr551] Antibody [NBP3-13261] - Untreated (-) and treated (+) Raji whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr551) antibody (NBP3-13261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BTK-[p-Tyr551]-Antibody-Western-Blot-NBP3-13261-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: BTK [p Tyr551] Antibody [NBP3-13261] - Untreated (-) and treated (+) Raji whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr551) antibody (NBP3-13261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: BTK Overexpression Lysate (Adult Normal) [NBP2-09059] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BTK.](http://images.novusbio.com/fullsize/BTK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09059-img0001.jpg "Western Blot: BTK Overexpression Lysate (Adult Normal) [NBP2-09059] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BTK.")