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NB110-89474

CD11b Antibody - BSA Free

(12 Reviews)

(104 Publications)

0.1 ml

NB110-89474

Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody (NB110-89474) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 488 (NB110-89474AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: CD11b Antibody - BSA Free [NB110-89474] - Western Blot detection of CD11b in RAW 264.7 whole cell lysates using [NB110-89474].

Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Staining of mouse pancreas using [NB110-89474] at 1:400 dilution. Nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). ICC/IF image submitted by a verified customer review.

Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Glibenclamide and Abcc8-/- suppress immune cell infiltration in EAE. White matter of lumbar spinal cord sections from WT control (a), untreated pid-30 WT/EAE (b), glibenclamide-treated pid-30 WT/EAE (c), and pid-30 Abcc8-/-/EAE (d) mice, stained with H&E or immunolabeled for CD45 (leucocyte), CD3 (T cells), CD20 (B cells), or CD11b (macrophage/microglia), as indicated; original magnification, x200 (H&E) or x400 (all immunolabelings). eleft panel: percent of quadrants with inflammatory cells on H&E; four mice/group. efour right panels: Quantification of CD-45-, CD3-, CD20-, and CD11b-expressing cells in white matter; four mice/group; ##P < 0.01 with respect to WT control; **P < 0.01, and ***P < 0.001 with respect to WT/EAE; scale bars, 100 um Image collected and cropped by CiteAb from the following publication (//www.jneuroinflammation.com/content/12/1/210) licensed under a CC-BY license.

Flow (Cell Surface): CD11b Antibody - BSA Free [NB110-89474] - Surface staining of CD11b in CT26 colorectal carcinoma tumor model. Using Alexa Fluor 405 conjugated version of the antibody (NB110-89474AF405). Image from verified customer review.

Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to Alexa Fluor 647 (NB110-89474AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - Raw264.7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD11b Antibody conjugated to DyLight 650 (NB110-8947C) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: CD11b Antibody - BSA Free [NB110-89474] - CD11b Antibody [NB110-89474] - CD11b antibody [NB110-89474] was tested in Raw264.7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and DyLight 550 (red).

Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Population of CD11b + myeloid progenitor cells differentiate into SMA + stromal cells within tumors and in vitro. Representative image of CD11b + SMA + double positive cells within tumor stroma. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32731354/) licensed under a CC-BY license.

Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Immunohistochemical analysis of CD11b in human renal cancer with [NB110-89474] using 3,3'-Diaminobenzidine (DAB) with hematoxylin counterstain.

Immunohistochemistry-Frozen: CD11b Antibody - BSA Free [NB110-89474] - Immunohistochemical analysis of frozen sections of mouse spleen using [NB110-89474]. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry: CD11b Antibody - BSA Free [NB110-89474] - Representative photomicrographs of cross sections of uteri collected from wild type (WT) mice 24 h after intrauterine infusion with 100 ul of vehicle or 100 ug ultrapure LPS from E. coli O111:B4, and Tlr4-/- mice similarly infused intrauterine with vehicle or LPS. Cross sections were stained with haematoxylin and eosin, and immunostained using a CD11b antibody for granulocytes (red) and DAPI for cell nuclei (white), and images collected at 10X magnification sections (C, F, I, L; bars = 200 um). At least 4 sections per animal and at least 4 fields per section were examined. Only WT mice infused with LPS show evidence of inflammation and accumulation of granulocytes in the endometrium. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0012906) licensed under a CC-BY license.

Flow (Cell Surface): CD11b Antibody - BSA Free [NB110-89474] - CD11b Antibody [NB110-89474] - A surface stain was performed on Raw264.7 cells with DyLight 550-conjugated [NB110-89474R] (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Flow (Cell Surface): CD11b Antibody - BSA Free [NB110-89474] - An intracellular stain was performed on THP-1 cells with CD11b NB110-89474 (blue) and a matched isotype control NBP2-24891 (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Simple Western: CD11b Antibody - BSA Free [NB110-89474] - Specific band for Cd11b in 1.0 mg/mL of Dentate Gyrus from Rat Brain. [NB110-89474] was used at a dilution of 1:50. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Simple Western image submitted by a verified customer review.

Dual RNAscope ISH-IHC: CD11b Antibody - BSA Free [NB110-89474] - Formalin-fixed paraffin-embedded tissue sections of human lymph node were probed for CD11b mRNA (ACD RNAScope Probe, catalog #555098; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog #NB110-89474) at 1:50 dilution with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.

Dual RNAscope ISH-IHC: CD11b Antibody - BSA Free [NB110-89474] - CD14 mRNA (red) and CD11b protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human malignant lymph node. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD14 and CD11b antibody at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNA scope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

CD11b staining in IMhu. Cells were stained with antibodies against the CD11b surface antigen. (A) Panel A shows positive staining for CD11b and (B) panel B shows nuclear DAPI staining. Magnitude 40×.

Glibenclamide and Abcc8-/- suppress immune cell infiltration in EAE. a-d White matter of lumbar spinal cord sections from WT control (a), untreated pid-30 WT/EAE (b), glibenclamide-treated pid-30 WT/EAE (c), and pid-30 Abcc8-/-/EAE (d) mice, stained with H&E or immunolabeled for CD45 (leucocyte), CD3 (T cells), CD20 (B cells), or CD11b (macrophage/microglia), as indicated; original magnification, x200 (H&E) or x400 (all immunolabelings). eleft panel: percent of quadrants with inflammatory cells on H&E; four mice/group. efour right panels: Quantification of CD-45-, CD3-, CD20-, and CD11b-expressing cells in white matter; four mice/group; ##P < 0.01 with respect to WT control; **P < 0.01, and ***P < 0.001 with respect to WT/EAE; scale bars, 100 um Image collected and cropped by CiteAb from the following publication (//www.jneuroinflammation.com/content/12/1/210), licensed under a CC-BY licence.

Population of CD11b + myeloid progenitor cells differentiate into SMA + stromal cells within tumors and in vitro. (C) CD11b + SMA + double positive cells within tumor stroma. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32731354), licensed under a CC-BY licence.

Intrauterine LPS stimulates inflammation in the endometrium of wild type but not Tlr4-/- mice.Representative photomicrographs of cross sections of uteri collected from wild type (WT) mice 24 h after intrauterine infusion with 100 ul of vehicle (A-C; n = 14) or 100 ug ultrapure LPS from E. coli O111:B4 (D-F; n = 17), and Tlr4-/- mice similarly infused intrauterine with vehicle (G-I; n = 8) or LPS (J-L; n = 8). Cross sections were stained with haematoxylin and eosin, and images collected at 10X magnification (A, D, G, J; bars = 200 um) or 40X magnification (B, E, H, K; bars = 50 um); or immunostained using a CD11b antibody for granulocytes (red) and DAPI for cell nuclei (white), and images collected at 10X magnification sections (C, F, I, L; bars = 200 um). At least 4 sections per animal and at least 4 fields per section were examined. Only WT mice infused with LPS show evidence of inflammation and accumulation of granulocytes in the endometrium (D-F). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0012906), licensed under a CC-BY licence.

Cell cycle block in PHD3 depleted cells is p27-dependent. a Reduced cell proliferation as judged by reduced cell amount of hypoxic siPHD3-depleted HeLa cells is rescued with concomitant p27 knockdown. b Quantification of cell survival from four independent experiments with 4–5 optical views per experiment. Asterisk indicates significant difference (p = 0,05; n = 4). Protein expression was monitored with western blotting from parallel samples. c Knockdown of p27 restores the effect of PHD3 depletion on cell cycle. HeLa cells exposed to the indicated double knockdown followed by 6 h hypoxia and FACS analysis. d Quantification of cell cycle phases from three independent experiments using indicated double knockdown Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26223520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Norgestrel reduces pro-inflammatory and promotes anti-inflammatory phenotypes in rd10 microglia.(A) RT-qPCR analysis detected similar levels of mRNAs encoding progesterone receptor membrane complexes 1 and 2 (PGRMC1, PGRMC2) and all three membrane progesterone receptor isoforms, alpha , beta and gamma (mPR alpha , mPR beta , mPR gamma ) in rd10 retinal microglial cells. Classical progesterone receptor (PR A/B) was not expressed. Actin, HPRT and GAPDH served as reference genes. (B) Immunolabeling confirmed the presence of these progesterone receptors in primary rd10 microglial cells. Hoechst (blue) staining reveals the cell nuclei. Scale bar represents 10μm. (C) Fluorescent microscopic images of primary rd10 microglia immunolabeled for (C) (i) markers of activation (CD68) and pro-inflammation (M1) (iNOS) and (C) (ii) anti-inflammatory activation (M2) (MRC1); after treatment with 20 μM Norgestrel over 24h. Norgestrel reduced pro-inflammatory and increased anti-inflammatory markers in rd10 microglia. Scale bar 10μm. (D) Quantification of fluorescence intensity of (D) (i) CD68 and iNOS and (D) (ii) MRC1 in primary rd10 microglial cultures following Norgestrel treatment over 24h. N = 8 mice, n = 4 technical replicates per group. (E) Nitrite concentration in the media of rd10 microglia in vitro treated with Norgestrel or vehicle over 24hr. Norgestrel significantly reduced nitrite release from rd10 microglia. N = 8 biological replicates, n = 2 technical replicates per sample. Results are presented as mean ± SEM (t-test, *p<0.05, ***p<0.005, ****p<0.001). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0165197), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Ce, ChHa, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ce, ChHa, Pm, RM

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-89474AF350
Alexa Fluor 405	NB110-89474AF405
Alexa Fluor 488	NB110-89474AF488
Alexa Fluor 532	NB110-89474AF532
Alexa Fluor 594	NB110-89474AF594
Alexa Fluor 647	NB110-89474AF647
Alexa Fluor 700	NB110-89474AF700
Alexa Fluor 750	NB110-89474AF750
Allophycocyanin	NB110-89474APC
Allophycocyanin/Cy7	NB110-89474APCCY7
Biotin	NB110-89474B
DyLight 350	NB110-89474UV
DyLight 405	NB110-89474V
DyLight 488	NB110-89474G
DyLight 550	NB110-89474R
DyLight 594	NB110-89474DL594
DyLight 650	NB110-89474C
DyLight 680	NB110-89474FR
DyLight 755	NB110-89474IR
FITC	NB110-89474F
HRP	NB110-89474H
Janelia Fluor 549	NB110-89474JF549
Janelia Fluor 646	NB110-89474JF646
PE	NB110-89474PE
PE/Atto594	NB110-89474PEATT594
PE/Cy5.5	NB110-89474PECY55
PE/Cy7	NB110-89474PECY7
PerCP	NB110-89474PCP
mFluor Violet 450 SE	NB110-89474MFV450
mFluor Violet 500 SE	NB110-89474MFV500
mFluor Violet 610 SE	NB110-89474MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-89474SS

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NB100-65284

CD11b Antibody (OX-42) - BSA Free

(1 Publication)

0.1 mg

NB100-65284

Flow Cytometry: CD11b Antibody (OX-42) [NB100-65284] - Detection of CD11b in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Rat CD11b Monoclonal Antibody (OX-42) [NB100-65284, filled histogram] or Mouse IgG2A Isotype Control Antibody [MAB003, open histogram] followed by anti-Mouse IgG PE-conjugated secondary antibody [F0102B] and Mouse Anti-Rat CD161 Alexa Fluor 647-conjugated Monoclonal Antibody [FAB9766R]. Splenocytes were gated on CD161+ cells.

Flow Cytometry: CD11b Antibody (OX-42) [NB100-65284] - Detection of CD11b in Rat Bone Marrow cells by Flow Cytometry. Rat cells were stained with Mouse Anti-Rat CD11b Monoclonal Antibody (OX-42) [NB100-65284, filled histogram] or Mouse IgG2A Isotype Control Antibody [MAB003, open histogram] followed by anti-Mouse IgG APC-conjugated secondary antibody [F0101B] .

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-65284AF350
Alexa Fluor 405	NB100-65284AF405
Alexa Fluor 488	NB100-65284AF488
Alexa Fluor 532	NB100-65284AF532
Alexa Fluor 594	NB100-65284AF594
Alexa Fluor 647	NB100-65284AF647
Alexa Fluor 700	NB100-65284AF700
Alexa Fluor 750	NB100-65284AF750
Allophycocyanin	NB100-65284APC
Allophycocyanin/Cy7	NB100-65284APCCY7
Biotin	NB100-65284B
DyLight 350	NB100-65284UV
DyLight 405	NB100-65284V
DyLight 488	NB100-65284G
DyLight 550	NB100-65284R
DyLight 594	NB100-65284DL594
DyLight 650	NB100-65284C
DyLight 680	NB100-65284FR
DyLight 755	NB100-65284IR
FITC	NB100-65284F
HRP	NB100-65284H
Janelia Fluor 549	NB100-65284JF549
Janelia Fluor 646	NB100-65284JF646
PE	NB100-65284PE
PE/Atto594	NB100-65284PEATT594
PE/Cy5.5	NB100-65284PECY55
PE/Cy7	NB100-65284PECY7
PerCP	NB100-65284PCP
mFluor Violet 450 SE	NB100-65284MFV450
mFluor Violet 500 SE	NB100-65284MFV500
mFluor Violet 610 SE	NB100-65284MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-65284SS
Azide and BSA Free	NBP2-80625

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NB500-608

CD34 Antibody (4H11[APG]) - BSA Free

0.1 mg

NB500-608

Immunocytochemistry/Immunofluorescence: CD34 Antibody (4H11[APG]) [NB500-608] - Human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE.

Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood

Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Analysis using the FITC conjugate of NB500-608. Staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. Total viable cells were used for analysis.

Flow Cytometry: CD34 Antibody (4H11[APG]) [NB500-608] - Staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor® 700.

Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Pacific BlueTM

Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) PE-CyTM7.

Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor® 488.

Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) APC.

Flow (Cell Surface): CD34 Antibody (4H11[APG]) [NB500-608] - Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (4H11[APG]) biotin / streptavidin-APC.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-608AF350
Alexa Fluor 405	NB500-608AF405
Alexa Fluor 488	NB500-608AF488
Alexa Fluor 532	NB500-608AF532
Alexa Fluor 594	NB500-608AF594
Alexa Fluor 647	NB500-608AF647
Alexa Fluor 700	NB500-608AF700
Alexa Fluor 750	NB500-608AF750
Allophycocyanin	NB500-608APC
Allophycocyanin/Cy7	NB500-608APCCY7
Biotin	NB500-608B
DyLight 350	NB500-608UV
DyLight 405	NB500-608V
DyLight 550	NB500-608R-0.1ml
DyLight 594	NB500-608DL594
DyLight 650	NB500-608C-0.1ml
DyLight 680	NB500-608FR
DyLight 755	NB500-608IR
FITC	NB500-608F
HRP	NB500-608H
Janelia Fluor 549	NB500-608JF549
Janelia Fluor 646	NB500-608JF646
PE	NB500-608PE
PE/Atto594	NB500-608PEATT594
PE/Cy5.5	NB500-608PECY55
PE/Cy7	NB500-608PECY7
PerCP	NB500-608PCP
mFluor Violet 450 SE	NB500-608MFV450
mFluor Violet 500 SE	NB500-608MFV500
mFluor Violet 610 SE	NB500-608MFV610

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