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![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - Sample: HeLa cells. Green: BMI1 protein stained by BMI1. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry--Immunofluorescence-NBP1-33748-img0016.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0025.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Western-Blot-NBP1-33661-img0011.jpg "Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-33661] - Analysis of methanol-fixed HeLa, using Syntenin 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33661-img0002.jpg)
![Immunohistochemistry-Frozen: Syntenin 1 Antibody [NBP1-33661] - Frozen Sectioned adult mouse brain. Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Frozen-NBP1-33661-img0010.jpg)
![Western Blot: Syntenin 1 Antibody [NBP1-33661] - Whole cell extracts (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Western-Blot-NBP1-33661-img0004.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0005.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0006.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Rat fore brain. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33661-img0007.jpg)
![Immunohistochemistry-Frozen: Syntenin 1 Antibody [NBP1-33661] - Frozen Sectioned adult mouse brain. Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Frozen-NBP1-33661-img0009.jpg)
![Western Blot: Syntenin 1 Antibody [NBP1-31136] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term (NBP1-31136). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Western-Blot-NBP1-31136-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Syntenin 1 Antibody [NBP1-31136] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term (NBP1-31136). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-31136] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Syntenin 1antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31136-img0011.jpg)
![Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-31136] - Mouse liver. Syntenin 1 antibody [N1], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31136-img0005.jpg)
![Western Blot: Syntenin 1 Antibody [NBP1-31136] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Western-Blot-NBP1-31136-img0006.jpg)
![Immunoprecipitation: Syntenin 1 Antibody [NBP1-31136] - Immunoprecipitation of Syntenin 1 protein from U87-MG whole cell extracts using 5 ug of Syntenin 1 antibody [N1], N-term (NBP1-31136). Western blot analysis was performed using Syntenin 1 antibody [N1], N-term (NBP1-31136).EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-Immunoprecipitation-NBP1-31136-img0009.jpg)
![Western Blot: PIASy Antibody [NBP1-31215] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human PIAS4-transfected 293T cells 10% SDS-PAGE PIASy antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PIASy-Antibody-Western-Blot-NBP1-31215-img0008.jpg "Western Blot: PIASy Antibody [NBP1-31215] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human PIAS4-transfected 293T cells 10% SDS-PAGE PIASy antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PIASy Antibody [NBP1-31215] - Paraformaldehyde-fixed HeLa, using PIASy antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31215-img0003.jpg)
![Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Mouse colon. PIASy antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunohistochemistry-NBP1-31215-img0006.jpg)
![Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Paraffin-embedded DLD1 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunohistochemistry-Paraffin-NBP1-31215-img0004.jpg)
![Western Blot: Tubulin Beta 4 Antibody [NBP1-32205] - Sample(30 ug of whole cell lysate) A:293T7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-Western-Blot-NBP1-32205-img0004.jpg "Western Blot: Tubulin Beta 4 Antibody [NBP1-32205] - Sample(30 ug of whole cell lysate) A:293T7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205] - Tubulin Beta 4 Antibody detects Tubulin Beta 4 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Tubulin Beta 4 stained by Tubulin Beta 4 Antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32205-img0005.jpg)
![Western Blot: Tubulin Beta 4 Antibody [NBP1-32205] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-Western-Blot-NBP1-32205-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205] - Paraformaldehyde-fixed U2OS, using beta Tubulin 4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Tubulin-Beta-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32205-img0002.jpg)
![Western Blot: Cytokeratin 2 Antibody [NBP1-31423] - Sample (30 ug of whole cell lysate) A: A431](http://images.novusbio.com/fullsize/Cytokeratin-2-Antibody-Western-Blot-NBP1-31423-img0005.jpg "Western Blot: Cytokeratin 2 Antibody [NBP1-31423] - Sample (30 ug of whole cell lysate) A: A431")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 2 Antibody [NBP1-31423] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31423-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 2 Antibody [NBP1-31423] - H661 xenograft, using KRT2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cytokeratin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31423-img0004.jpg)
![Western Blot: PIP5K1 alpha Antibody [NBP2-19833] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PIP5K1-alpha-Antibody-Western-Blot-NBP2-19833-img0005.jpg "Western Blot: PIP5K1 alpha Antibody [NBP2-19833] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PIP5K1 alpha Antibody [NBP2-19833] - Immunofluorescence analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PIP5K1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19833-img0003.jpg)
![Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-19833] - CL1-0 xenograft, using PIPK I alpha antibody at 1:100 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PIP5K1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19833-img0002.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20761] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20761-img0001.jpg "Western Blot: Tubulin alpha-1B Antibody [NBP2-20761] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20761] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using alpha Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20761-img0003.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20761] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-20761-img0002.jpg)
![Western Blot: OASL Antibody [NBP2-19618] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/OASL-Antibody-Western-Blot-NBP2-19618-img0001.jpg "Western Blot: OASL Antibody [NBP2-19618] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:2000.")
![Immunocytochemistry/Immunofluorescence: OASL Antibody [NBP2-19618] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/OASL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19618-img0003.jpg)
![Immunohistochemistry-Paraffin: OASL Antibody [NBP2-19618] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/OASL-Antibody-Immunohistochemistry-Paraffin-NBP2-19618-img0002.jpg)
![Western Blot: ACTR3 Antibody [NBP2-15277] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ACTR3 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ACTR3-Antibody-Western-Blot-NBP2-15277-img0003.jpg "Western Blot: ACTR3 Antibody [NBP2-15277] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ACTR3 antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ACTR3 Antibody [NBP2-15277] - Human gastric cancer, using Arp3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ACTR3-Antibody-Immunohistochemistry-Paraffin-NBP2-15277-img0001.jpg)
![Western Blot: ACTR3 Antibody [NBP2-15277] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ACTR3-Antibody-Western-Blot-NBP2-15277-img0002.jpg)
![Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508] - IP samples: HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunoprecipitation-NBP2-16508-img0003.jpg "Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508] - IP samples: HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Western Blot: Flotillin-1 Antibody [NBP2-16508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp2-16508_rabbit-polyclonal-flotillin-1-antibody-western-blot-8620239419..jpg)
![Western Blot: RHOBTB1 Antibody [NBP2-20155] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/RHOBTB1-Antibody-Western-Blot-NBP2-20155-img0001.jpg "Western Blot: RHOBTB1 Antibody [NBP2-20155] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:5000.")