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![Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).](http://images.novusbio.com/fullsize/Cdc2-[p-Tyr15]-Antibody-Western-Blot-NB500-142-img0001.jpg "Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).")
![Western Blot: CDK2 [p Tyr 15] Antibody (SR1560) [NBP3-21552] - Western blot analysis using NBP3-21552 in (1) HeLa treated with AP; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21552_rabbit-cdk2-p-tyr-15-mab-sr1560-57202317215182.jpg "Western Blot: CDK2 [p Tyr 15] Antibody (SR1560) [NBP3-21552] - Western blot analysis using NBP3-21552 in (1) HeLa treated with AP; (2) HeLa cell lysate.")
![Immunohistochemistry: CDK2 [p Tyr 15] Antibody (SR1560) [NBP3-21552] - Immunohistochemical analysis of paraffin-embedded human bladder cancer, using NBP3-21552.](http://images.novusbio.com/fullsize/nbp3-21552_rabbit-cdk2-p-tyr-15-mab-sr1560-5720231564720.jpg)
![Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-262202413221513.jpg "Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).")
![Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate (treated with Pervanadate or not). All lanes: CDK2 [p Tyr15] Antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.Predicted band size: 34 KDaObserved band size: 34 KDa](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-28220241140949.jpg)
![Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Analysis of CDK2 [p Tyr15] Antibody (2C4) diluted at 1:80 and staining in Hela cells (treated with Pervanadate). The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-28220241312220.jpg)
![Western Blot: Cdk5 [p Tyr15] Antibody [NBP3-12917] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cdk5 (phospho Tyr15) antibody (NBP3-12917) diluted at 1:4000.](http://images.novusbio.com/fullsize/Cdk5-[p-Tyr15]-Antibody-Western-Blot-NBP3-12917-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Cdk5 [p Tyr15] Antibody [NBP3-12917] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cdk5 (phospho Tyr15) antibody (NBP3-12917) diluted at 1:4000.")