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![Western Blot: c-Myb [p Thr486] Antibody [NBP2-21659] - Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and treated with okadaic acid to include phosphorylation of c-Myb. Load: 35 ug per lane. The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4C. Secondary antibody: Dye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: PBS-Tween overnight at 4C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb: wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.](http://images.novusbio.com/fullsize/c-Myb-[p-Thr486]-Antibody-Western-Blot-NBP2-21659-img0001.jpg "Western Blot: c-Myb [p Thr486] Antibody [NBP2-21659] - Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and treated with okadaic acid to include phosphorylation of c-Myb. Load: 35 ug per lane. The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4C. Secondary antibody: Dye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: PBS-Tween overnight at 4C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb: wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.")