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![Western Blot: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3). Using NBP2-61546, showed a band of Histone H3 phosphorylated at threonine 3 in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Western-Blot-NBP2-61546-img0007.jpg "Western Blot: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3). Using NBP2-61546, showed a band of Histone H3 phosphorylated at threonine 3 in HeLa cells. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunocytochemistry of HeLa cells using NBP2-61546 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Immunocytochemistry-Immunofluorescence-NBP2-61546-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunocytochemistry of HeLa cells using NBP2-61546 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Immunocytochemistry-Immunofluorescence-NBP2-61546-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using NBP2-61546 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-131020231935490.png)
![Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202411472125.jpg "Western Blot: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: Histone H3.3 [p Thr3] Antibody at 1.41 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa Observed band size: 16 KDa")
![Immunocytochemistry/Immunofluorescence: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Analysis of Histone H3.3 [p Thr3] Antibody (28H4) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-28220241323624.jpg)
![Flow Cytometry: Histone H3.3 [p Thr3] Antibody (28H4) [NBP3-26418] - Overlay histogram showing Hela cells stained with Histone H3.3 [p Thr3] Antibody (28H4) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26418_rabbit-histone-h3-3-p-thr-3-mab-28h4-282202414183663.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0003.jpg "Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Histone H3 (phospho Thr3) antibody detects Histone H3 (phospho Thr3) protein by western blot analysis. Un-treated (-) and treated (+, 0.1uM Calyculin for 30 minutes + phosphatase inhibitor) HeLa whole cell extracts (2 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0001.jpg)
![Western Blot: Histone H3 [p Thr3] Antibody [NBP3-12929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody (NBP3-12929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr3]-Antibody-Western-Blot-NBP3-12929-img0002.jpg)