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![Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Immunohistochemistry-NB100-2371-img0003.jpg "Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.")
![Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - NBF-fixed asynchronous HeLa cells. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-Aurora A. Antibody: Affinity purified rabbit anti- Phospho-Aurora A (T288) (NB100-2371) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (NB7159) used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2371-img0002.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")