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![Western Blot: actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody [NBP3-23382] - Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23382_rabbit-actin-related-protein-2-3-complex-subunit-1b-p-thr21-pab-199202312523618.jpg "Western Blot: actin-related protein 2/3 complex subunit 1B [p Thr21] Antibody [NBP3-23382] - Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).")
![Western Blot: RPA2 [p Thr21] Antibody [NBP3-12924] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPA2 (phospho Thr21) antibody (NBP3-12924) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/RPA2-[p-Thr21]-Antibody-Western-Blot-NBP3-12924-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPA2 [p Thr21] Antibody [NBP3-12924] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPA2 (phospho Thr21) antibody (NBP3-12924) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: RPA2 [p Thr21] Antibody [NBP3-12924] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPA2 (phospho Thr21) antibody (NBP3-12924) diluted at 1:1000.](http://images.novusbio.com/fullsize/RPA2-[p-Thr21]-Antibody-Western-Blot-NBP3-12924-img0001.jpg)
![Western Blot: RPA2 [p Thr 21] Antibody (SR1535) [NBP3-21543] - Western blot analysis using NBP3-21543 in HeLa cell lysate treated with Calyculin A.](http://images.novusbio.com/fullsize/nbp3-21543_rabbit-rpa2-p-thr-21-mab-sr1535-57202317161847.jpg "Western Blot: RPA2 [p Thr 21] Antibody (SR1535) [NBP3-21543] - Western blot analysis using NBP3-21543 in HeLa cell lysate treated with Calyculin A.")
![Immunocytochemistry/Immunofluorescence: RPA2 [p Thr21] Antibody (3B2) [NBP3-26396] - Staining of Hela cells with RPA2 [p Thr21] Antibody (3B2) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26396_rabbit-rpa2-p-thr-21-mab-3b2-282202412493635.jpg "Immunocytochemistry/Immunofluorescence: RPA2 [p Thr21] Antibody (3B2) [NBP3-26396] - Staining of Hela cells with RPA2 [p Thr21] Antibody (3B2) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).")